Mouse Monoclonal Lambda Free Light Chain antibody. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (AB1944), expandable thumbnail](https://content.abcam.com/products/images/lambda-free-light-chain-antibody-3d12-ab1944--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89984.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (AB1944)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Recommended products
Mouse Monoclonal Lambda Free Light Chain antibody. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 3D12
- Purification technique
- Affinity purification Protein A
- Specificity
Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.
- Concentration
- Purification notes
Purity tested by electrophoresis.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Concentration varies from lot to lot and can be provided on request.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lambda Free Light Chain also known as lambda light chain or lambda FLC represents an essential component of immunoglobulin molecules. These polypeptide chains typically have a molecular weight of approximately 22.5 kDa. Lambda free light chains are expressed by B cells in the bone marrow and lymphoid tissues. They eventually appear in the bloodstream where they circulate as part of the broader immune response.
- Biological function summary
Free lambda light chains contribute to the assembly of immunoglobulin molecules by forming dimers with heavy chains. They participate in the adaptive immune system's ability to identify and neutralize pathogens. Lambda FLCs operate as part of immunoglobulin G (IgG) complexes which enhance immune response efficiency. When synthesized in excess they are secreted into the bloodstream helping mediate various immune functions.
- Pathways
Lambda FLC plays a role in the antibody-mediated immune response and complement activation pathways. In these pathways lambda light chains interact with other immunoglobulin molecules to initiate pathogen opsonization and clearance. Connections with proteins like immunoglobulin heavy chain exemplify their involvement in complex immune cascades that defend the host against infections.
- Associated diseases and disorders
An elevated level of lambda free light chains often indicates multiple myeloma or light chain amyloidosis. In multiple myeloma abnormal plasma cells produce excess lambda FLC disrupting normal blood cell production. The condition associates with proteins such as Bence Jones proteins composed of excess light chains that can be detected in urine and serve as important diagnostic markers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944), expandable thumbnail](https://content.abcam.com/products/images/lambda-free-light-chain-antibody-3d12-ab1944--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89984.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)
IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Downloads
Product protocols
- Visit the general protocols
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