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AB1944

Anti-Lambda Free Light Chain antibody [3D12]

0

(1 Review)

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(1 Publication)

Mouse Monoclonal Lambda Free Light Chain antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (AB1944)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (AB1944)

IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1944, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3D12

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Specificity

Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.

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Complementary Products

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Primary Antibodies

AB134929

Anti-Human Kappa Chain antibody [EPR5539-71-8]

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Primary Antibodies

AB187370

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Secondary Antibodies

AB99811

Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Product details

Concentration varies from lot to lot and can be provided on request.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purity tested by electrophoresis.
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lambda Free Light Chain also known as lambda light chain or lambda FLC represents an essential component of immunoglobulin molecules. These polypeptide chains typically have a molecular weight of approximately 22.5 kDa. Lambda free light chains are expressed by B cells in the bone marrow and lymphoid tissues. They eventually appear in the bloodstream where they circulate as part of the broader immune response.
Biological function summary

Free lambda light chains contribute to the assembly of immunoglobulin molecules by forming dimers with heavy chains. They participate in the adaptive immune system's ability to identify and neutralize pathogens. Lambda FLCs operate as part of immunoglobulin G (IgG) complexes which enhance immune response efficiency. When synthesized in excess they are secreted into the bloodstream helping mediate various immune functions.

Pathways

Lambda FLC plays a role in the antibody-mediated immune response and complement activation pathways. In these pathways lambda light chains interact with other immunoglobulin molecules to initiate pathogen opsonization and clearance. Connections with proteins like immunoglobulin heavy chain exemplify their involvement in complex immune cascades that defend the host against infections.

An elevated level of lambda free light chains often indicates multiple myeloma or light chain amyloidosis. In multiple myeloma abnormal plasma cells produce excess lambda FLC disrupting normal blood cell production. The condition associates with proteins such as Bence Jones proteins composed of excess light chains that can be detected in urine and serve as important diagnostic markers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Lambda Free Light Chain
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 10:4611 PubMed32165720

2020

Abundant human anti-Galα3Gal antibodies display broad pathogen reactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Jens Magnus Bernth Jensen,Mikkel Steen Petersen,Svend Ellerman-Eriksen,Bjarne Kuno Møller,Jens Christian Jensenius,Uffe B Skov Sørensen,Steffen Thiel
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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