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Rabbit Recombinant Monoclonal LV151 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples. Cited in 3 publications.

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Images

Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719), expandable thumbnail
  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719), expandable thumbnail
  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PELISAWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Tested
Recombinant fragment - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/64000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20000
Notes

For unpurified use at 1/50000 - 1/200000.

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

For unpurified use at 1/500 - 1/1000.

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Alternative names

Immunoglobulin lambda variable 1-51, Ig lambda chain V-I region BL2, Ig lambda chain V-I region EPS, Ig lambda chain V-I region NEW, Ig lambda chain V-I region NIG-64, IGLV1-51

Recommended products

Rabbit Recombinant Monoclonal LV151 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5367-62
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Lambda Light Chain also known as lambda chain or lambda protein forms a part of immunoglobulins (antibodies). Its mass typically ranges between 22 to 24 kDa. This protein is expressed primarily in B cells. Specifically it pairs with heavy chains to form the complete antibody molecule. It participates in the antigen recognition process supporting the immune system's defense mechanisms.

Biological function summary

Lambda Light Chain participates in immunological functions by contributing to the antigen-binding site of antibodies. It exists as a part of the immunoglobulin complex. The lambda chain alongside heavy chains forms the variable part of antibodies allowing specificity in antigen recognition. Its variation leads to a diverse antibody repertoire which is important for recognizing a wide range of pathogens.

Pathways

Lambda Light Chain fits into pathways involving adaptive immune responses. It is important in antigen processing and presentation pathways. In this context it interacts with the heavy chains which together drive the specificity and efficiency of immune response pathways. It also associates with proteins like the major histocompatibility complex (MHC) which facilitates antigen presentation to T cells.

Associated diseases and disorders

Lambda Light Chain associates with multiple myeloma a cancer of plasma cells and light chain amyloidosis a disorder where light chains accumulate as amyloid fibrils in tissues. In these conditions proteins like immunoglobulin heavy chain and albumin often interact with or are affected by lambda chains providing insights into disease mechanisms and potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling Lambda Light chain with unpurified ab124719 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719), expandable thumbnail

    Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/100000 dilution

    All lanes: Human plasma lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa

    Observed band size: 25 kDa

  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719), expandable thumbnail

    Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/20000 dilution

    All lanes: Human thymus tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa

    Observed band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lambda Light chain with purified ab124719 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling Lambda Light chain with purified ab124719 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719), expandable thumbnail

    ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    ELISA analysis of Human Kappa light chain (Free), Human Lambda Light Chains (Free), Human IgA, Human IgM, Human IgG, Rat IgG, Mouse IgG at 1000 ng/mL with ab124719 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719), expandable thumbnail

    Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    All lanes: Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/50000 dilution

    Lane 1: Human tonsil lysate at 10 µg

    Lane 2: Human plasma lysate at 10 µg

    Lane 3: Human spleen lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 23 kDa, 49 kDa, 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Lambda Light chain with unpurified ab124719 at a dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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