AB124719

Anti-Lambda Light chain antibody [EPR5367-62]

RabMAb
Recombinant
20ul selling size
What is this?

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(7 Publications)

Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) is a rabbit monoclonal antibody detecting Lambda Light chain in Western Blot, Flow Cytometry (Intra), IHC-P, ICC/IF, ELISA. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Immunoglobulin lambda variable 1-51, Ig lambda chain V-I region BL2, Ig lambda chain V-I region EPS, Ig lambda chain V-I region NEW, Ig lambda chain V-I region NIG-64, IGLV1-51

8 Images

Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling Lambda Light chain with unpurified ab124719 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling Lambda Light chain with purified ab124719 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.

Control 1 : primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lambda Light chain with purified ab124719 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Lambda Light chain with unpurified ab124719 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

ELISA

Lab

ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

ELISA analysis of Human Kappa light chain (Free), Human Lambda Light Chains (Free), Human IgA, Human IgM, Human IgG, Rat IgG, Mouse IgG at 1000 ng/mL with ab124719 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Lab

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/20000 dilution

All lanes:

Human thymus tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

false

Lab

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/100000 dilution

All lanes:

Human plasma lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

false

Unknown

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

All lanes:

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/50000 dilution

Lane 1:

Human tonsil lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 23 kDa,49 kDa,61 kDa

false

Carrier free

Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free
HRP

HRP Anti-Lambda Light chain antibody [EPR5367-62]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5367-62

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB, ICC/IF, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "For unpurified use at 1/50000 - 1/200000.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "For unpurified use at 1/500 - 1/1000.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/64000", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human samples.

What is the molecular weight of Lambda Light chain?
Anti-Lambda Light chain [EPR5367-62] (ab124719) specifically detects a band for Lambda Light chain (UniProt: P0CG04) at a molecular weight of 25kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5367-62] also available for your convenience: ab124719, Carrier free - ab185131, HRP - ab200966

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lambda Light Chain also known as lambda chain or lambda protein forms a part of immunoglobulins (antibodies). Its mass typically ranges between 22 to 24 kDa. This protein is expressed primarily in B cells. Specifically it pairs with heavy chains to form the complete antibody molecule. It participates in the antigen recognition process supporting the immune system's defense mechanisms.

Biological function summary

Lambda Light Chain participates in immunological functions by contributing to the antigen-binding site of antibodies. It exists as a part of the immunoglobulin complex. The lambda chain alongside heavy chains forms the variable part of antibodies allowing specificity in antigen recognition. Its variation leads to a diverse antibody repertoire which is important for recognizing a wide range of pathogens.

Pathways

Lambda Light Chain fits into pathways involving adaptive immune responses. It is important in antigen processing and presentation pathways. In this context it interacts with the heavy chains which together drive the specificity and efficiency of immune response pathways. It also associates with proteins like the major histocompatibility complex (MHC) which facilitates antigen presentation to T cells.

Lambda Light Chain associates with multiple myeloma a cancer of plasma cells and light chain amyloidosis a disorder where light chains accumulate as amyloid fibrils in tissues. In these conditions proteins like immunoglobulin heavy chain and albumin often interact with or are affected by lambda chains providing insights into disease mechanisms and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed : 24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGLV1-51

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1636162 PubMed41019035

2025

Monoclonal immunoglobulins on red blood cells: a potential supplementary diagnostic indicator for monoclonal gammopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Benxia Bing,Xiaowei Yang,Chenghua Wang,Liang Xu,Shiqing Cheng,Yun Liu,Qiuhan Wang,Chunjuan Zhai,Bing Zhao,Rong Wang,Jing Sun

Scientific reports 15:4292 PubMed39905088

2025

Amyloidogenic immunoglobulin light chains disturb contractile function and calcium transients in a human cardiac spheroid model of light chain (AL) amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mélanie Bézard,Jean-Sébastien Vartanian-Grimaldi,Julien Henri,Denisa Calin,Amira Zaroui,Mounira Kharoubi,Thibaud Damy,Onnik Agbulut,Ekaterini Kordeli

Nature communications 14:3897 PubMed37400446

2023

A rapid cell-free expression and screening platform for antibody discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew C Hunt,Bastian Vögeli,Ahmed O Hassan,Laura Guerrero,Weston Kightlinger,Danielle J Yoesep,Antje Krüger,Madison DeWinter,Michael S Diamond,Ashty S Karim,Michael C Jewett

Signal transduction and targeted therapy 7:289 PubMed36008393

2022

The heterogeneous immune landscape between lung adenocarcinoma and squamous carcinoma revealed by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Chengdi Wang,Qiuxiao Yu,Tingting Song,Zhoufeng Wang,Lujia Song,Ying Yang,Jun Shao,Jingwei Li,Yinyun Ni,Ningning Chao,Li Zhang,Weimin Li

Molecular medicine reports 23: PubMed33760139

2021

Expression of immunoglobulin G in human proximal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenling Deng,Ziyang Jing,Yanhong Guo,Junfan Ma,Huige Yan,Zhan Shi,Hui Deng,Yaoxian Liang,Song Wang,Zhuan Cui,Yuejuan Pan,Xiaoyan Qiu,Yue Wang

International journal of molecular medicine 41:3296-3306 PubMed29512722

2018

Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyang Jing,Hui Deng,Junfan Ma,Yanhong Guo,Yaoxian Liang,Rui Wu,Lata A,Zihan Geng,Xiaoyan Qiu,Yue Wang

Cancer letters 339:49-59 PubMed23887058

2013

Hsp70 inhibition induces myeloma cell death via the intracellular accumulation of immunoglobulin and the generation of proteotoxic stress.

Applications

Species

Human

Lei Zhang,Jacqueline J L Fok,Fabio Mirabella,Lauren I Aronson,Rosemary A Fryer,Paul Workman,Gareth J Morgan,Faith E Davies

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lambda Light chain antibody [EPR5367-62]

Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (AB124719)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise