Rabbit Recombinant Monoclonal LV151 antibody. Carrier free. Suitable for IHC-P, ELISA, ICC/IF, Flow Cyt (Intra), WB and reacts with Human, Recombinant full length protein - Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--flow-cytometry-intracellular-img37348.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114461.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--immunocytochemistry-immunofluorescence-img22429.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131)")
![ELISA - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--elisa-img22369.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136935.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (AB185131)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ELISA | ICC/IF | Flow Cyt (Intra) | WB | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
1 product for Alternative Version
Target data
Function
V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin lambda variable 1-51, Ig lambda chain V-I region BL2, Ig lambda chain V-I region EPS, Ig lambda chain V-I region NEW, Ig lambda chain V-I region NIG-64, IGLV1-51
Recommended products
Rabbit Recombinant Monoclonal LV151 antibody. Carrier free. Suitable for IHC-P, ELISA, ICC/IF, Flow Cyt (Intra), WB and reacts with Human, Recombinant full length protein - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5367-62
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab185131 is the carrier-free version of Anti-Lambda Light chain antibody [EPR5367-62] ab124719.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lambda Light Chain also known as lambda chain or lambda protein forms a part of immunoglobulins (antibodies). Its mass typically ranges between 22 to 24 kDa. This protein is expressed primarily in B cells. Specifically it pairs with heavy chains to form the complete antibody molecule. It participates in the antigen recognition process supporting the immune system's defense mechanisms.
- Biological function summary
Lambda Light Chain participates in immunological functions by contributing to the antigen-binding site of antibodies. It exists as a part of the immunoglobulin complex. The lambda chain alongside heavy chains forms the variable part of antibodies allowing specificity in antigen recognition. Its variation leads to a diverse antibody repertoire which is important for recognizing a wide range of pathogens.
- Pathways
Lambda Light Chain fits into pathways involving adaptive immune responses. It is important in antigen processing and presentation pathways. In this context it interacts with the heavy chains which together drive the specificity and efficiency of immune response pathways. It also associates with proteins like the major histocompatibility complex (MHC) which facilitates antigen presentation to T cells.
- Associated diseases and disorders
Lambda Light Chain associates with multiple myeloma a cancer of plasma cells and light chain amyloidosis a disorder where light chains accumulate as amyloid fibrils in tissues. In these conditions proteins like immunoglobulin heavy chain and albumin often interact with or are affected by lambda chains providing insights into disease mechanisms and potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--flow-cytometry-intracellular-img37348.jpg "Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)")
Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)
Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling Lambda Light chain with unpurified Anti-Lambda Light chain antibody [EPR5367-62] ab124719 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lambda Light chain antibody [EPR5367-62] ab124719).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114461.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lambda Light chain with purified Anti-Lambda Light chain antibody [EPR5367-62] ab124719 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lambda Light chain antibody [EPR5367-62] ab124719).
![Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--immunocytochemistry-immunofluorescence-img22429.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)")
Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)
Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling Lambda Light chain with purified Anti-Lambda Light chain antibody [EPR5367-62] ab124719 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/250) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lambda Light chain antibody [EPR5367-62] ab124719).
![ELISA - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--elisa-img22369.jpg "ELISA - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)")
ELISA - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)
ELISA analysis of Human Kappa light chain (Free), Human Lambda Light Chains (Free), Human IgA, Human IgM, Human IgG, Rat IgG, Mouse IgG at 1000 ng/mL with Anti-Lambda Light chain antibody [EPR5367-62] ab124719 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lambda Light chain antibody [EPR5367-62] ab124719).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131), expandable thumbnail](https://content.abcam.com/products/images/lambda-light-chain-antibody-epr5367-62-bsa-and-azide-free-ab185131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136935.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] - BSA and Azide free (ab185131)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Lambda Light chain with unpurified Anti-Lambda Light chain antibody [EPR5367-62] ab124719 at a dilution of 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lambda Light chain antibody [EPR5367-62] ab124719).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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