AB210959

Anti-LAMC2 antibody [CL2980]

Name: Anti-LAMC2 antibody [CL2980] (ab210959) | Abcam
Brand: Abcam Limited
SKU: AB210959
Price: 470 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(21 Publications)

Mouse Monoclonal LAMC2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LAMC2 aa 1050 to C-terminus.

View Alternative Names

LAMB2T, LAMNB2, LAMC2, Laminin subunit gamma-2, Cell-scattering factor 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma, CSF 140 kDa subunit

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon tissue labeling LAMC2 with ab210959 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lymph node tissue labeling LAMC2 with ab210959 at 1/500 dilution (negative control).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human cervix tissue labeling LAMC2 with ab210959 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [CL2980] (AB210959)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [CL2980] (AB210959)

PFA-fixed, Triton X-100 permeabilized A431 cells stained for LAMC2 (Green) using ab210959 at 4 μg/ml in ICC/IF.

Microtublues are stained red, nuclei are stained blue.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human stomach tissue labeling LAMC2 with ab210959 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate tissue labeling LAMC2 with ab210959 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human skin tissue labeling LAMC2 with ab210959 at 1/500 dilution.

Supplier Data

Western blot - Anti-LAMC2 antibody [CL2980] (AB210959)

All lanes:

Western blot - Anti-LAMC2 antibody [CL2980] (ab210959) at 1/500 dilution

Lane 1:

Purified Human recombinant protein LAMC2

Lane 2:

Purified Human recombinant protein Laminin-421

Lane 3:

Purified Human recombinant protein Laminin-511

Lane 4:

Purified Human recombinant protein Laminin-121

Lane 5:

Purified Human recombinant protein Laminin-221

Predicted band size: 131 kDa

false

Supplier Data

Western blot - Anti-LAMC2 antibody [CL2980] (AB210959)

All lanes:

Western blot - Anti-LAMC2 antibody [CL2980] (ab210959) at 1/500 dilution

All lanes:

A431 lysate

Predicted band size: 131 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2980

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LAMC2 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13753

Epitope

Binds to an epitope located within the peptide sequence IQDTLNTLDGLLHLM as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilisation: PFA/Triton X-100" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LAMC2 protein also known as laminin subunit gamma-2 is an essential component of the extracellular matrix. It is a subunit of the laminin-332 complex formerly referred to as laminin-5 and has an approximate mass of 160 kDa. LAMC2 is widely expressed in various tissues most prominently in epithelial tissues. It localizes at the basement membrane zone where epithelial and mesenchymal tissues meet.

Biological function summary

LAMC2 plays a critical role in cell adhesion migration and signal transduction. Laminin-332 which includes LAMC2 binds to integrin receptors on cell surfaces facilitating cell-matrix interactions and communication. This complex is important for basement membrane stability and tissue organization and it is actively involved in wound healing by promoting keratinocyte migration and melanin transport.

Pathways

LAMC2 integrates into several important extracellular matrix pathways including the integrin signaling pathway. These pathways involve interactions with proteins like integrin alpha-6/beta-4 which are linked to cellular adhesion and mobility. LAMC2 also contributes to epithelial cell functions and is involved in the PI3K/AKT signaling pathway highlighting its importance in cell proliferation and survival.

LAMC2 has significant associations with conditions like dystrophic epidermolysis bullosa and invasive carcinomas. In dystrophic epidermolysis bullosa mutations in LAMC2 disrupt the integrity of the basement membrane leading to fragile skin and blistering. In cancer abnormal expression or alteration of LAMC2 can promote tumor invasion and metastasis often in conjunction with overexpression of matrix metalloproteinases (MMPs) that degrade the extracellular matrix therefore facilitating cancer cell infiltration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.

See full target information LAMC2

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 9: PubMed40366121

2025

Single-cell transcription reveals hepatocyte-to-cholangiocyte reprogramming and biliary gene profile in biliary atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdu Meng,Min Du,Haodong Li,Fanyang Kong,Jiajian Yang,Rui Dong,Shan Zheng,Gong Chen,Zhen Shen,Junfeng Wang

International journal of oral science 17:29 PubMed40240323

2025

Programmed death-ligand 1 regulates ameloblastoma growth and recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Linzhou Zhang,Hao Lin,Jiajie Liang,Xuanhao Liu,Chenxi Zhang,Qiwen Man,Ruifang Li,Yi Zhao,Bing Liu

International journal of molecular sciences 26: PubMed40244200

2025

Mechanism of Tumor Budding in Patient-Derived Metachronous Oral Primary Squamous Cell Carcinoma Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Omae,Yuji Omori,Yuna Makihara,Koji Yamanegi,Soutaro Hanawa,Kyohei Yoshikawa,Kazuma Noguchi,Hiromitsu Kishimoto

Biomedicines 12: PubMed39595099

2024

NSUN2 Promotes Head and Neck Squamous Cell Carcinoma Progression by Targeting EMT-Related Gene LAMC2 in an mC-YBX1-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Shuojin Huang,Congyuan Cao,Dongxiao Tang,Yiwen Liu,Wanhang Zhou,Lianlian Liu,Xin Zheng,Qianting He,Anxun Wang

Molecular therapy. Oncology 32:200858 PubMed39280586

2024

Galunisertib promotes bevacizumab-induced vascular normalization in nasopharyngeal carcinoma: Multi-parameter MRI evaluation.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Xia Du,Shuai Zhang,Jinhua Long,Peng Wu,Zongxue Li,Xinyue Lyu,Qin Hong,Pengyu Chen,Bo Gao

International journal of molecular sciences 25: PubMed39201392

2024

TNF Induces Laminin-332-Encoding Genes in Endothelial Cells and Laminin-332 Promotes an Atherogenic Endothelial Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Assim Hayderi,Mulugeta Melkie Zegeye,Sare Meydan,Allan Sirsjö,Ashok Kumar Kumawat,Liza U Ljungberg

Journal for immunotherapy of cancer 12: PubMed38908857

2024

Understanding the dynamics of TKI-induced changes in the tumor immune microenvironment for improved therapeutic effect.

Applications

Unspecified application

Species

Unspecified reactive species

Conghua Lu,Ziyuan Gao,Di Wu,Jie Zheng,Chen Hu,Daijuan Huang,Chao He,Yihui Liu,Caiyu Lin,Tao Peng,Yuanyao Dou,Yimin Zhang,Fenfen Sun,Weiling Jiang,Guoqing Yin,Rui Han,Yong He

Heliyon 10:e31299 PubMed38803944

2024

Bulk and single-cell RNA sequencing reveal the contribution of laminin γ2 -CD44 to the immune resistance in lymphocyte-infiltrated squamous lung cancer subtype.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Song,Ying Yang,Yilong Wang,Yinyun Ni,Yongfeng Yang,Li Zhang

Aging 16:7902-7914 PubMed38709270

2024

Improvement of skin wound healing by giant salamander skin mucus gel wrapped with bone marrow mesenchymal stem cells via affecting integrin family molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Dayong Du,Yan Huang,Cui Xu,Yang Liu,Xiaohong Wu,Jie Yang,Zhipeng Liu,Jianxin Ma,Chaoji Huangfu

Journal of cancer research and clinical oncology 150:230 PubMed38703300

2024

LAMC2 regulates the proliferation, invasion, and metastasis of gastric cancer via PI3K/Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Cheng,Xiaofei Li,Wenhui Dong,Jing Yang,Pengmei Li,Xihui Qiang,Jiajun Yin,Lianyi Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMC2 antibody [CL2980]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMC2 antibody [CL2980] (AB210959)

Western blot - Anti-LAMC2 antibody [CL2980] (AB210959)

Western blot - Anti-LAMC2 antibody [CL2980] (AB210959)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

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