AB281016

Anti-LAMC2 antibody [EPR23654-74] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal LAMC2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

LAMB2T, LAMNB2, LAMC2, Laminin subunit gamma-2, Cell-scattering factor 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma, CSF 140 kDa subunit

1 Images

Sandwich ELISA - Anti-LAMC2 antibody [EPR23654-74] - BSA and Azide free (Detector) (AB281016)

sELISA

Supplier Data

Sandwich ELISA - Anti-LAMC2 antibody [EPR23654-74] - BSA and Azide free (Detector) (AB281016)

Example of Human LAMC2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23654-74

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab281016 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LAMC2. The recommended pair for sandwich ELISA is:
Capture: ab281166, Human LAMC2 Capture Antibody (unconjugated)
Detector: ab281016, Human LAMC2 Detector Antibody (unconjugated)
The reference range value is 31.25 - 200 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LAMC2 protein also known as laminin subunit gamma-2 is an essential component of the extracellular matrix. It is a subunit of the laminin-332 complex formerly referred to as laminin-5 and has an approximate mass of 160 kDa. LAMC2 is widely expressed in various tissues most prominently in epithelial tissues. It localizes at the basement membrane zone where epithelial and mesenchymal tissues meet.

Biological function summary

LAMC2 plays a critical role in cell adhesion migration and signal transduction. Laminin-332 which includes LAMC2 binds to integrin receptors on cell surfaces facilitating cell-matrix interactions and communication. This complex is important for basement membrane stability and tissue organization and it is actively involved in wound healing by promoting keratinocyte migration and melanin transport.

Pathways

LAMC2 integrates into several important extracellular matrix pathways including the integrin signaling pathway. These pathways involve interactions with proteins like integrin alpha-6/beta-4 which are linked to cellular adhesion and mobility. LAMC2 also contributes to epithelial cell functions and is involved in the PI3K/AKT signaling pathway highlighting its importance in cell proliferation and survival.

LAMC2 has significant associations with conditions like dystrophic epidermolysis bullosa and invasive carcinomas. In dystrophic epidermolysis bullosa mutations in LAMC2 disrupt the integrity of the basement membrane leading to fragile skin and blistering. In cancer abnormal expression or alteration of LAMC2 can promote tumor invasion and metastasis often in conjunction with overexpression of matrix metalloproteinases (MMPs) that degrade the extracellular matrix therefore facilitating cancer cell infiltration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.

See full target information LAMC2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMC2 antibody [EPR23654-74] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-LAMC2 antibody [EPR23654-74] - BSA and Azide free (Detector) (AB281016)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise