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Mouse Monoclonal Lamin-A/C antibody. Suitable for Flow Cyt, WB, IHC-Fr, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 99 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LMNA.


Images

Flow Cytometry - Anti-Lamin A antibody [133A2] (AB8980), expandable thumbnail
  • Western blot - Anti-Lamin A antibody [133A2] (AB8980), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (AB8980), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody [133A2] (AB8980), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (AB8980), expandable thumbnail

Publications

Key facts

Isotype

IgG3

Host species

Mouse

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human LMNA. The exact immunogen used to generate this antibody is proprietary information. Database link P02545

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-FrICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/200.00000

Notes

ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

1/100.00000 - 1/200.00000

Notes

ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

PMID: 9274531

Expected
Expected

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

PMID: 9274531

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/100.00000 - 1/200.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/100.00000 - 1/200.00000

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Lamin-A/CLamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:2188730, PubMed:22431096, PubMed:2344612, PubMed:23666920, PubMed:24741066, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:24741066, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:23990565, PubMed:25127216). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2188730, PubMed:2344612). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920).Prelamin-A/CPrelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).

Alternative names

Recommended products

Mouse Monoclonal Lamin-A/C antibody. Suitable for Flow Cyt, WB, IHC-Fr, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 99 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LMNA.

Key facts

Isotype

IgG3

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human LMNA. The exact immunogen used to generate this antibody is proprietary information. Database link P02545
Clone number

133A2

Purification technique

Ion exchange chromatography

Specificity

Reacts against lamin A exclusively (the antibody was raised against the carboxy-terminus of 98 amino acids present in lamin A and absent from lamin C. As a result, this antibody recognizes lamin A but not lamin C; See Machiels et al 1997). Lamins do not appear to be universally distributed among different cell and tissue types. ab8980 has been tested in testis parenchyma and testicular germ cell tumours (See Machiels et al 1997). Other cell/tissue types have not been tested.

Epitope

Recognizes an epitope located between residues 598-611 of lamin A.

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

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8 product images

  • Flow Cytometry - Anti-Lamin A antibody [133A2] (ab8980), expandable thumbnail

    Flow Cytometry - Anti-Lamin A antibody [133A2] (ab8980)

    Overlay histogram showing HeLa cells stained with ab8980 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8980, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

  • Western blot - Anti-Lamin A antibody [133A2] (ab8980), expandable thumbnail

    Western blot - Anti-Lamin A antibody [133A2] (ab8980)

    Lanes 1 - 4: Merged signal (red and green). Green - ab8980 observed at 74 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    ab8980 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in LMNA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LMNA knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. ab8980 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Lamin A antibody [133A2] (ab8980) at 1/100 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: LMNA knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: HepG2 whole cell lysate at 20 µg

    Predicted band size: 74 kDa

    Observed band size: 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (ab8980), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (ab8980)

    ICC/IF image of ab8980 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (Normal Goat Serum ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8980 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody [133A2] (ab8980), expandable thumbnail
    This image is courtesy of a customer review submitted by Mr Carl Hobbs

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody [133A2] (ab8980)

    ab8980 staining of Lamin A in adult mouse liver sections. Formaldehyde-fixed paraffin-embedded sections of mouse liver tissue were incubated with ab8980 (1/2500) fo 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0 (10x Citrate Buffer pH 6.0 ab64214). Please see accompanying abreview for additional information.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (ab8980), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (ab8980)

    These images show HeLa cells stained with Laminin A antibody - ab8980 (green) and with DAPI (blue). The pictures were kindly supplied as part of the review submitted by Dr Josef Gotzmann at Medical University of Vienna, Austria.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody [133A2] (ab8980), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody [133A2] (ab8980)

    Immunohistochemistry on paraffin section of human colon.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin A antibody [133A2] (ab8980), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Lamin A antibody [133A2] (ab8980)

    Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (ab8980), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] (ab8980)

    Immunocytochemical staining of fiboblasts showing nuclear lamina

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Product protocols

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