Anti-Lamin A antibody [133A2] - Nuclear Envelope Marker

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody [133A2] - Nuclear Envelope Marker (AB8980)

Immunohistochemistry on paraffin section of human colon.

• Flow Cyt

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Flow Cytometry - Anti-Lamin A antibody [133A2] - Nuclear Envelope Marker (AB8980)

Overlay histogram showing HeLa cells stained with ab8980 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8980, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] - Nuclear Envelope Marker (AB8980)

These images show HeLa cells stained with Laminin A antibody - ab8980 (green) and with DAPI (blue). The pictures were kindly supplied as part of the review submitted by Dr Josef Gotzmann at Medical University of Vienna, Austria.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Lamin A antibody [133A2] - Nuclear Envelope Marker (AB8980)

Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] - Nuclear Envelope Marker (AB8980)

ICC/IF image of ab8980 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8980 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• IHC-P

AbReview10872****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A antibody [133A2] - Nuclear Envelope Marker (AB8980)

ab8980 staining of Lamin A in adult mouse liver sections. Formaldehyde-fixed paraffin-embedded sections of mouse liver tissue were incubated with ab8980 (1/2500) fo 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0 (ab64214). Please see accompanying abreview for additional information.

This image is courtesy of an Abreview submitted by Mr Carl Hobbs

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody [133A2] - Nuclear Envelope Marker (AB8980)

Immunocytochemical staining of fibroblasts showing nuclear lamina.