Rabbit Polyclonal Lamin-A/C antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 117 publications.
Images
Publications
- Cells 12:2023Mineralocorticoid Receptor Antagonism Prevents Type 2 Familial Partial Lipodystrophy Brown Adipocyte Dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Schena,Elisabetta Mattioli,Chiara Peres,Laura Zanotti,Paolo Morselli,Patricia Iozzo,Maria Angela Guzzardi,Chiara Bernardini,Monica Forni,Salvatore Nesci,Massimiliano Caprio,Carolina Cecchetti,Uberto Pagotto,Elena Gabusi,Luca Cattini,Gina Lisignoli,William Blalock,Alessandra Gambineri,Giovanna LattanziPubMed 37998321
- Nature communications 14:31012023The nuclear lamina couples mechanical forces to cell fate in the preimplantation embryo via actin organization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobin M Skory,Adam A Moverley,Goli Ardestani,Yanina Alvarez,Ana Domingo-Muelas,Oz Pomp,Blake Hernandez,Piotr Tetlak,Stephanie Bissiere,Claudio D Stern,Denny Sakkas,Nicolas PlachtaPubMed 37248263
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Chicken | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Lamin-A/CLamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:2344612, PubMed:2188730, PubMed:24741066, PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:24741066, PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:25127216, PubMed:23990565). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2344612, PubMed:2188730). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:23666920, PubMed:18611980).Prelamin-A/CPrelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).
Alternative names
Prelamin-A/C, LMNA, LMN1
Recommended products
Rabbit Polyclonal Lamin-A/C antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 117 publications.
Alternative names
Prelamin-A/C, LMNA, LMN1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
Replenishment batches of ab26300 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
Lamin A also known as LMNA is a type of lamin protein integral to the nuclear architecture. It has a molecular weight of approximately 70 kDa. This protein localizes mainly to the nuclear lamina a dense fibrillar network inside the inner nuclear membrane found across various cell types. Lamin A serves as a structural scaffold mediating nuclear stability and rigidity. It also plays a role in chromatin organization supporting overall nuclear functions.
- Biological function summary
The lamin molecule interacts with several nuclear components forming part of the nuclear lamina complex. It anchors chromatin material and regulates DNA replication and repair. Lamin A protein also modulates gene expression through interactions with transcription factors. Beyond structural support it influences cell cycle progression and differentiation impacting cellular mechanotransduction and signaling processes.
- Pathways
Lamin A performs critical functions within the cell cycle and apoptotic pathways. Its interactions with the retinoblastoma protein (pRB) and other cyclin-dependent kinases control cell cycle checkpoints and progression. Moreover lamin A connects with proteins involved in signaling pathways like MAPK which relate to stress responses and cellular growth. These interactions highlight its dynamic involvement in maintaining cell health and proliferation.
- Associated diseases and disorders
Mutations in the lamin A gene associate closely with Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. In these conditions altered lamin A protein affects nuclear shape and chromatin layout disrupting transcriptional regulation. The mutated lamin A protein interacts differently with binding partners like emerin contributing to muscle adipose tissue and multi-system abnormalities. This highlights the critical role lamin A plays in maintaining normal cellular function across life stages.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
This image is courtesy of an anonymous customer review.Western blot - Anti-Lamin A antibody (ab26300)
Blocking: 5% milk for 30 minutes at 22°C
All lanes: Western blot - Anti-Lamin A antibody (ab26300) at 1/1000 dilution
All lanes: HeLa whole cell extract at 100 µg
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L) HRP Conjugate at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 74 kDa
Observed band size: 76 kDa
Exposure time: 15s
Western blot - Anti-Lamin A antibody (ab26300)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Lamin A knockout HAP1 cell lysate (20 μg)
Lane 3: A431 cell lysate (20 μg)
Lane 4: NIH3T3 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab26300 observed at 76 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab26300 was shown to recognize Lamin A in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Lamin A knockout samples were examined. Wild-type and Lamin A knockout samples were subjected to SDS-PAGE. ab26300 1ug/ml and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) at a dilution of 1/1000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Lamin A antibody (ab26300)
Predicted band size: 74 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Lamin A antibody (ab26300)
All lanes: Western blot - Anti-Lamin A antibody (ab26300) at 1/1000 dilution
Lane 1: Mouse NIH-3T3 cells - cytosolic fraction at 25 µg
Lane 2: Mouse NIH-3T3 cells - nuclear fraction at 25 µg
SecondaryAll lanes: HRP conjugated Goat anti-rabbit at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 76 kDa
Exposure time: 10s
Western blot - Anti-Lamin A antibody (ab26300)
All lanes: Western blot - Anti-Lamin A antibody (ab26300) at 1 µg/mL
All lanes: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 68 kDa, 76 kDa
Western blot - Anti-Lamin A antibody (ab26300)
All lanes: Western blot - Anti-Lamin A antibody (ab26300) at 1 µg/mL
Lane 1: Western blot - Recombinant Human Lamin A protein (Recombinant Human Lamin A protein ab83472) at 0.1 µg
Lane 2: Western blot - Recombinant Human Lamin A protein (Recombinant Human Lamin A protein ab83472) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Exposure time: 10s
Western blot - Anti-Lamin A antibody (ab26300)
All lanes: Western blot - Anti-Lamin A antibody (ab26300) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 100 kDa, 45 kDa, 70 kDa, 74 kDa
Western blot - Anti-Lamin A antibody (ab26300)
Lysates at 20 µg per lane.
The samples were run on a Bis-Tris gel under reducing conditions.
Western blot: Anti-Lamin A + Lamin C antibody (Anti-Lamin A + Lamin C antibody [RM1093] ab315838) staining at 1/1000 dilution or Anti-Lamin A antibody (ab26300) at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5](Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-Lamin A + Lamin C antibody [RM1093] ab315838 was shown to bind specifically to Lamin A + Lamin C. Target of interest was observed at 70-75 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in LMNA knockout cell line (lane 2, knockout cell line Human LMNA (Lamin A) knockout HeLa cell line ab261787/knockout cell lysate Human LMNA (Lamin A) knockout HeLa cell lysate ab256979). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.
All lanes: Western blot - Anti-Lamin A + Lamin C antibody [RM1093] (Anti-Lamin A + Lamin C antibody [RM1093] ab315838) at 1/1000 dilution
Lane 1: Wild-type Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: LMNA knockout Hela whole cell lysate at 20 µg
Lane 3: HeG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 70-75 kDa, 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Lamin A antibody (ab26300)
ab26300 staining Lamin A in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab26300 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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