Rabbit Recombinant Monoclonal Lamin B1 antibody. Carrier free. Suitable for WB, IP, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
WB | IP | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected | Expected | Tested |
Rat | Tested | Expected | Expected | Expected | Tested |
Ictidomys tridecemlineatus | Predicted | Predicted | Predicted | Predicted | Predicted |
Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human, Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
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Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
LMNA, LMNA
Lamin-B1, LMNB, LMN2, LMNB1
Rabbit Recombinant Monoclonal Lamin B1 antibody. Carrier free. Suitable for WB, IP, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR4068
Affinity purification Protein A
The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.
Blue Ice
+4°C
Do Not Freeze
ab226043 is the carrier-free version of Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Lamin A Lamin B1 and Lamin C are nuclear envelope proteins each representing different forms of lamin molecules. These proteins are important components of the nuclear lamina. Lamin A and Lamin C are encoded by the LMNA gene and result from alternative splicing. Lamin A has a molecular weight of approximately 74 kDa while Lamin C weighs around 65 kDa. Lamin B1 with a molecular weight of about 66 kDa is a product of the LMNB1 gene. These proteins express largely in various tissues and are central to maintaining nuclear structure and integrity.
Lamin proteins regulate nuclear architecture and maintain chromatin organization. They participate in forming the nuclear lamina a dense fibrillar network inside the nucleus. The lamina provides structural support to the nuclear envelope and plays roles in DNA replication and transcription. Lamin molecules also interact with nuclear pore complexes therefore contributing to molecular transport across the nuclear envelope. Lamin A/C and Lamin B1 function together with other proteins like emerin and lamin associated polypeptides in forming multi-protein complexes that influence cell cycle regulation and differentiation.
Lamin proteins integrate into pathways governing the cell cycle and DNA repair. They contribute to the mitotic pathway assisting in the disassembly and reassembly of the nuclear envelope during cell division. Lamin molecules also engage in the DNA damage response pathway cooperating with repair proteins such as p53 and BRCA1. Through these pathways lamin proteins ensure proper progression through the cell cycle and maintain genomic stability by aiding in the repair of DNA damage.
Lamins associate with a variety of nuclear envelopathies and arepsponsible for rare genetic disorders like Hutchinson-Gilford Progeria Syndrome (HGPS) and Emery-Dreifuss Muscular Dystrophy (EDMD). Mutations in the LMNA gene affect nuclear mechanics leading to accelerated aging in HGPS. Similarly disruptions in lamin A/C's interactions with emerin result in EDMD characterized by muscular dystrophy and cardiac issues. These connections highlight the role of lamin proteins in nuclear stability and their impact on disease development.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 (purified) at 1/20 immunoprecipitating Lamin A + C in 10 �g HepG2 (Lanes 1 and 2, observed at 70, 74, and 65 kDa - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 recognises three isoforms). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
All lanes: Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922)
Immunofluorescence staining of HeLa cells with purified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
Immunohistochemical staining of paraffin embedded rat liver with purified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
Intracellular Flow Cytometry analysis of HeLa cells labelling Lamin A + B1 + C with purified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour 488®-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
Immunohistochemical staining of paraffin embedded human liver carcinoma with purified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
Immunohistochemical staining of paraffin embedded mouse liver with purified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
Immunohistochemical analysis of paraffin-embedded human colonic tissue using unpurified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 at a diltion of 1/100
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
Unpurified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 showing positive staining in Normal human breast tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
Unpurified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 showing positive staining in Normal uterus tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
Unpurified Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922 showing positive staining in Normal kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] ab108922).
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