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Rabbit Recombinant Monoclonal Lamin B1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 34 publications.


Images

Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922), expandable thumbnail
  • Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (AB108922), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (AB108922), expandable thumbnail
  • Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Tested
Ictidomys tridecemlineatus
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Ictidomys tridecemlineatus
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Ictidomys tridecemlineatus
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Ictidomys tridecemlineatus
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Ictidomys tridecemlineatus
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Ictidomys tridecemlineatus
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:28716252, PubMed:32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:28716252, PubMed:32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:28716252, PubMed:32910914).

Additional Targets

LMNA, LMNA

Alternative names

LMN2, LMNB, LMNB1, Lamin-B1

Recommended products

Rabbit Recombinant Monoclonal Lamin B1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 34 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4068
Purification technique
Affinity purification Protein A
Specificity

The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Lamin A Lamin B1 and Lamin C are nuclear envelope proteins each representing different forms of lamin molecules. These proteins are important components of the nuclear lamina. Lamin A and Lamin C are encoded by the LMNA gene and result from alternative splicing. Lamin A has a molecular weight of approximately 74 kDa while Lamin C weighs around 65 kDa. Lamin B1 with a molecular weight of about 66 kDa is a product of the LMNB1 gene. These proteins express largely in various tissues and are central to maintaining nuclear structure and integrity.

Biological function summary

Lamin proteins regulate nuclear architecture and maintain chromatin organization. They participate in forming the nuclear lamina a dense fibrillar network inside the nucleus. The lamina provides structural support to the nuclear envelope and plays roles in DNA replication and transcription. Lamin molecules also interact with nuclear pore complexes therefore contributing to molecular transport across the nuclear envelope. Lamin A/C and Lamin B1 function together with other proteins like emerin and lamin associated polypeptides in forming multi-protein complexes that influence cell cycle regulation and differentiation.

Pathways

Lamin proteins integrate into pathways governing the cell cycle and DNA repair. They contribute to the mitotic pathway assisting in the disassembly and reassembly of the nuclear envelope during cell division. Lamin molecules also engage in the DNA damage response pathway cooperating with repair proteins such as p53 and BRCA1. Through these pathways lamin proteins ensure proper progression through the cell cycle and maintain genomic stability by aiding in the repair of DNA damage.

Associated diseases and disorders

Lamins associate with a variety of nuclear envelopathies and arepsponsible for rare genetic disorders like Hutchinson-Gilford Progeria Syndrome (HGPS) and Emery-Dreifuss Muscular Dystrophy (EDMD). Mutations in the LMNA gene affect nuclear mechanics leading to accelerated aging in HGPS. Similarly disruptions in lamin A/C's interactions with emerin result in EDMD characterized by muscular dystrophy and cardiac issues. These connections highlight the role of lamin proteins in nuclear stability and their impact on disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

  • Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)

    Lamin A + Lamin B1 + Lamin C Western blot staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/10000 dilution

    Lane 1: mouse heart lysate at 10 µg

    Lane 2: rat heart lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

    Predicted band size: 130 kDa, 30 kDa, 47 kDa

    Observed band size: 65 kDa, 70 kDa, 74 kDa

  • Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail

    Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)

    ab108922 (purified) at 1/20 immunoprecipitating Lamin A + B1 + C in 10 μg HepG2 (Lanes 1 and 2, observed at 70, 74, and 65 kDa - ab108922 recognises three isoforms). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Immunofluorescence staining of HeLa cells with purified ab108922 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108922 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Immunohistochemical staining of paraffin embedded rat liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)

    Lamin A + Lamin B1 + Lamin C Western blot staining of GST tagged recombinant Human Lamin B1 protein (1 to 586) (91 KDa) using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    All lanes: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/1000 dilution

    Lane 1: Western blot - Recombinant Human Lamin A protein (Recombinant Human Lamin A protein ab83472) at 10 µg

    Lane 2: GST tagged recombinant Human Lamin B1 protein (1 to 586) (91 KDa) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75 kDa, 91 kDa

    Exposure time: 180s

  • Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)

    Lamin A + Lamin B1 + Lamin C Western blot staining of Myc and DDK tagged recombinant Human Lamin B2 protein using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    ab108922 does not cross react with Lamin B2.

    Lane 1: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/1000 dilution

    Lane 2: Anti-Myc tag antibody at 1/10000 dilution

    Lane 3: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/5000 dilution

    All lanes: Myc and DDK tagged recombinant Human Lamin B2 protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 68 kDa

    Exposure time: 7s

  • Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Intracellular Flow Cytometry analysis of HeLa cells labelling Lamin A + B1 + C with purified ab108922 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)

    Lamin A + Lamin B1 + Lamin C Western blot staining of HeLa cell lysate using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/10000 dilution

    All lanes: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

    Observed band size: 65 kDa, 70 kDa, 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Immunohistochemical staining of paraffin embedded human liver carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Immunohistochemical staining of paraffin embedded mouse liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Immunohistochemical analysis of paraffin-embedded human colonic tissue using unpurified ab108922 at a diltion of 1/100

  • Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)

    Lamin A + Lamin B1 + Lamin C Western blot staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody

    All lanes: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: HACAT cell lysate at 10 µg

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Unpurified ab108922 showing positive staining in Normal human breast tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Unpurified ab108922 showing positive staining in Normal human uterus tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

    Unpurified ab108922 showing positive staining in Normal human kidney tissue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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