Rabbit Recombinant Monoclonal Lamin B1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 34 publications.
Images
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img83580.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)")
![Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunoprecipitation-img8851.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunocytochemistry-immunofluorescence-img14156.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104669.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)")
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img129841.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)")
Publications
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- International journal of molecular sciences 24:2023Epigenetic Downregulation of Hsa-miR-193b-3p Increases Cyclin D1 Expression Level and Cell Proliferation in Human Meningiomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaulina Kober,Beata Joanna Mossakowska,Natalia Rusetska,Szymon Baluszek,Emilia Grecka,Ryszard Konopiński,Ewa Matyja,Artur Oziębło,Tomasz Mandat,Mateusz BujkoPubMed 37686289
- Bone research 11:352023Premature aging of skeletal stem/progenitor cells rather than osteoblasts causes bone loss with decreased mechanosensation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuici Yang,Dandan Cao,Jinlong Suo,Lingli Zhang,Chunyang Mo,Miaomiao Wang,Ningning Niu,Rui Yue,Weiguo ZouPubMed 37407584
- Nature neuroscience 26:737-7502023Tau activation of microglial cGAS-IFN reduces MEF2C-mediated cognitive resilience.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoe C Udeochu,Sadaf Amin,Yige Huang,Li Fan,Eileen Ruth S Torres,Gillian K Carling,Bangyan Liu,Hugo McGurran,Guillermo Coronas-Samano,Grant Kauwe,Gergey Alzaem Mousa,Man Ying Wong,Pearly Ye,Ravi Kumar Nagiri,Iris Lo,Julia Holtzman,Carlo Corona,Allan Yarahmady,Michael T Gill,Ravikiran M Raju,Sue-Ann Mok,Shiaoching Gong,Wenjie Luo,Mingrui Zhao,Tara E Tracy,Rajiv R Ratan,Li-Huei Tsai,Subhash C Sinha,Li GanPubMed 37095396
- British journal of pharmacology 180:1056-10712022Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWupeng Liao,Hazel Yu Ci Foo,Thi Ngoc Quy Tran,Christina Li Lin Chai,Wai Shiu Fred WongPubMed 36440573
- iScience 25:1053522022Manganese enhances DNA- or RNA-mediated innate immune response by inducing phosphorylation of TANK-binding kinase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Sui,Qian Chen,Jun Yang,Selena Srirattanapirom,Tomozumi ImamichiPubMed 36325059
- Cellular and molecular gastroenterology and hepatology 15:121-1522022PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuey-Huey Chua,Mei-Hwei Chang,Ya-Hui Chen,Daw-Jen Tsuei,Yung-Ming Jeng,Po-Huang Lee,Yen-Hsuan NiPubMed 36191855
- Genes 13:2022MiR-18a-5p Targets Connective Tissue Growth Factor Expression and Inhibits Transforming Growth Factor β2-Induced Trabecular Meshwork Cell Contractility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn Knox,George Bou-Gharios,Kevin J Hamill,Colin E WilloughbyPubMed 36011411
- Cell reports 39:1107892022The oocyte spindle midzone pauses Cdk1 inactivation during fertilization to enable male pronuclear formation and embryo development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenxi Zhou,Hayden A HomerPubMed 35508138
- Journal of thrombosis and haemostasis : JTH 19:2480-24942021Fibrinogen activates focal adhesion kinase (FAK) promoting colorectal adenocarcinoma growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBal Krishan Sharma,Duaa Mureb,Sumit Murab,Leah Rosenfeldt,Brenton Francisco,Rachel Cantrell,Rebekah Karns,Lindsey Romick-Rosendale,Miki Watanabe-Chailland,Jacob Mast,Matthew J Flick,Patrick W Whitlock,Joseph S PalumboPubMed 34192410
- Nature communications 12:202021The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangjian Fan et. al.PubMed 33397932
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected | Tested |
| Rat | Expected | Tested | Expected | Expected | Tested |
| Ictidomys tridecemlineatus | Predicted | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Ictidomys tridecemlineatus | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:28716252, PubMed:32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:28716252, PubMed:32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:28716252, PubMed:32910914).
Additional Targets
LMNA, LMNA
Alternative names
LMN2, LMNB, LMNB1, Lamin-B1
Recommended products
Rabbit Recombinant Monoclonal Lamin B1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 34 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4068
- Purification technique
- Affinity purification Protein A
- Specificity
The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lamin A Lamin B1 and Lamin C are nuclear envelope proteins each representing different forms of lamin molecules. These proteins are important components of the nuclear lamina. Lamin A and Lamin C are encoded by the LMNA gene and result from alternative splicing. Lamin A has a molecular weight of approximately 74 kDa while Lamin C weighs around 65 kDa. Lamin B1 with a molecular weight of about 66 kDa is a product of the LMNB1 gene. These proteins express largely in various tissues and are central to maintaining nuclear structure and integrity.
- Biological function summary
Lamin proteins regulate nuclear architecture and maintain chromatin organization. They participate in forming the nuclear lamina a dense fibrillar network inside the nucleus. The lamina provides structural support to the nuclear envelope and plays roles in DNA replication and transcription. Lamin molecules also interact with nuclear pore complexes therefore contributing to molecular transport across the nuclear envelope. Lamin A/C and Lamin B1 function together with other proteins like emerin and lamin associated polypeptides in forming multi-protein complexes that influence cell cycle regulation and differentiation.
- Pathways
Lamin proteins integrate into pathways governing the cell cycle and DNA repair. They contribute to the mitotic pathway assisting in the disassembly and reassembly of the nuclear envelope during cell division. Lamin molecules also engage in the DNA damage response pathway cooperating with repair proteins such as p53 and BRCA1. Through these pathways lamin proteins ensure proper progression through the cell cycle and maintain genomic stability by aiding in the repair of DNA damage.
- Associated diseases and disorders
Lamins associate with a variety of nuclear envelopathies and arepsponsible for rare genetic disorders like Hutchinson-Gilford Progeria Syndrome (HGPS) and Emery-Dreifuss Muscular Dystrophy (EDMD). Mutations in the LMNA gene affect nuclear mechanics leading to accelerated aging in HGPS. Similarly disruptions in lamin A/C's interactions with emerin result in EDMD characterized by muscular dystrophy and cardiac issues. These connections highlight the role of lamin proteins in nuclear stability and their impact on disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
16 product images
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img83580.jpg "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Western blot staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/10000 dilution
Lane 1: mouse heart lysate at 10 µg
Lane 2: rat heart lysate at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution
Predicted band size: 130 kDa, 30 kDa, 47 kDa
Observed band size: 65 kDa, 70 kDa, 74 kDa
![Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunoprecipitation-img8851.jpg "Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
ab108922 (purified) at 1/20 immunoprecipitating Lamin A + B1 + C in 10 μg HepG2 (Lanes 1 and 2, observed at 70, 74, and 65 kDa - ab108922 recognises three isoforms). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunocytochemistry-immunofluorescence-img14156.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Immunofluorescence staining of HeLa cells with purified ab108922 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108922 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104669.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Immunohistochemical staining of paraffin embedded rat liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img129841.jpg "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Western blot staining of GST tagged recombinant Human Lamin B1 protein (1 to 586) (91 KDa) using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Blocking/Diluting buffer and concentration 5% NFDM/TBST
All lanes: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/1000 dilution
Lane 1: Western blot - Recombinant Human Lamin A protein (Recombinant Human Lamin A protein ab83472) at 10 µg
Lane 2: GST tagged recombinant Human Lamin B1 protein (1 to 586) (91 KDa) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 75 kDa, 91 kDa
Exposure time: 180s
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img94337.jpg "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Western blot staining of Myc and DDK tagged recombinant Human Lamin B2 protein using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
ab108922 does not cross react with Lamin B2.
Lane 1: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/1000 dilution
Lane 2: Anti-Myc tag antibody at 1/10000 dilution
Lane 3: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/5000 dilution
All lanes: Myc and DDK tagged recombinant Human Lamin B2 protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 68 kDa
Exposure time: 7s
![Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--flow-cytometry-intracellular-img2951.jpg "Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Flow Cytometry (Intracellular) staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Intracellular Flow Cytometry analysis of HeLa cells labelling Lamin A + B1 + C with purified ab108922 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img155023.jpg "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Western blot staining of HeLa cell lysate using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/10000 dilution
All lanes: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution
Observed band size: 65 kDa, 70 kDa, 74 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24320.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41705.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Immunohistochemical staining of paraffin embedded human liver carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132469.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Immunohistochemical staining of paraffin embedded mouse liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85611.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Immunohistochemical analysis of paraffin-embedded human colonic tissue using unpurified ab108922 at a diltion of 1/100
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img35434.jpg "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Western blot staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
All lanes: Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HACAT cell lysate at 10 µg
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128798.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Unpurified ab108922 showing positive staining in Normal human breast tissue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112305.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Unpurified ab108922 showing positive staining in Normal human uterus tissue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132979.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)
Lamin A + Lamin B1 + Lamin C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Lamin A + Lamin B1 + Lamin C antibody
Unpurified ab108922 showing positive staining in Normal human kidney tissue.
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img94337.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--flow-cytometry-intracellular-img2951.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img155023.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24320.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41705.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132469.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85611.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--western-blot-img35434.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128798.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112305.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922), expandable thumbnail](https://content.abcam.com/products/images/lamin-a-lamin-b1-lamin-c-antibody-epr4068-nuclear-envelope-marker-ab108922--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132979.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (ab108922)")
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