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Anti-Lamin A + Lamin C antibody [4C11] (ab238303) is a mouse monoclonal antibody that is used to detect Lamin A + Lamin C in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse samples.



- Specificity confirmed with Lamin A + Lamin C knockout cell line validation


Images

Western blot - Anti-Lamin A + Lamin C antibody [4C11] (AB238303), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [4C11] (AB238303), expandable thumbnail
  • Western blot - Anti-Lamin A + Lamin C antibody [4C11] (AB238303), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [4C11] (AB238303), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [4C11] (AB238303), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
0.1 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Lamin-A/C. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:2188730, PubMed:22431096, PubMed:2344612, PubMed:23666920, PubMed:24741066, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:24741066, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:23990565, PubMed:25127216). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2188730, PubMed:2344612). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920). Prelamin-A/C. Prelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).

Additional Targets

LMNA

Alternative names

LMN1, LMNA, Prelamin-A/C

Recommended products

Anti-Lamin A + Lamin C antibody [4C11] (ab238303) is a mouse monoclonal antibody that is used to detect Lamin A + Lamin C in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse samples.



- Specificity confirmed with Lamin A + Lamin C knockout cell line validation

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
4C11
Purification technique
Affinity purification Protein G
Epitope
The 4C11 monoclonal recognizes the Ig-fold domain of lamin A and lamin C (PubMed ID: 20498701).
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Knockout and wild type cell lines have been used to confirm the specificity of this antibody in ICC/IF and WB.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Lamin A and Lamin C also known as lamin A/C are proteins encoded by the LMNA gene. These proteins are key components of the nuclear envelope where they provide structural support and maintain the shape of the nucleus. The lamin A/C molecule has a molecular weight of approximately 60-70 kDa. Expression of lamin A and lamin C occurs predominantly in differentiated cells where these proteins integrate into the nuclear lamina alongside other lamin molecules like lamin B. Lamin A alone sometimes referred to by designations like 4C11 plays a significant role in mechanical support at a molecular level.

Biological function summary

Lamin A/C proteins play a role in maintaining nuclear stability chromosome organization and gene regulation. They are part of a complex network within the nuclear lamina that includes interactions with proteins and DNA. Lamin A with a molecular weight distinct from other lamins participates in assembling this supportive matrix and contributes to DNA maintenance and repair processes. Their interaction with chromatin and gene expression regulation emphasizes their influence on important cellular functions.

Pathways

Lamin A/C proteins engage in the mechanosensory signaling and DNA damage response pathways. They interact with pathways involving the nuclear envelope structure and have connections to proteins like emerin and nuclear actin. Lamin A's role in these pathways supports its involvement in responding to mechanical stress and preserving genomic integrity highlighting its integration with these cellular processes.

Associated diseases and disorders

Mutations in lamin A/C are linked to disorders such as Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. These conditions highlight the importance of lamin A/C in cellular stability and nuclear integrity. Proteins such as emerin often relate to lamin A/C in these diseases as disruptions to their interactions can lead to compromised nuclear function and disease phenotypes.

Product promise

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5 product images

  • Western blot - Anti-Lamin A + Lamin C antibody [4C11] (ab238303), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C antibody [4C11] (ab238303)

    Lanes 1-2: Merged signal (red and green). Green - ab238303 observed at 74 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa.

    ab238303 Anti-Lamin A + C antibody [4C11] was shown to specifically react with Lamin A + C in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human LMNA (Lamin A) knockout HeLa cell line ab261787 (knockout cell lysate Human LMNA (Lamin A) knockout HeLa cell lysate ab256979) was used. Wild-type and Lamin A + C knockout samples were subjected to SDS-PAGE. ab238303 and Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4°C at 1 μg/ml and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Lamin A + Lamin C antibody [4C11] (ab238303) at 1 µg/mL

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: Lamin A + C knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human LMNA (Lamin A) knockout HeLa cell line (Human LMNA (Lamin A) knockout HeLa cell line ab261787)

    Secondary

    All lanes: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 74 kDa

    Observed band size: 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [4C11] (ab238303), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [4C11] (ab238303)

    ab238303 staining Lamin A+C (colored green) in wild-type HAP1 cells (top panel) and LMNA knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab238303 at 1μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution overnight at 4°C. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Lamin A + Lamin C antibody [4C11] (ab238303), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C antibody [4C11] (ab238303)

    ab238303 was shown to specifically react with Lamin A + C (LMNA) in wild type HAP1 cells. No band was observed when Lamin A + C (LMNA) knockout samples were used. Wild-type and Lamin A + C (LMNA) knockout samples were subjected to SDS-PAGE. ab238303 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Lamin A + Lamin C antibody [4C11] (ab238303)

    Lane 1: HAP1 whole cell lysate at 20 µg

    Lane 2: HAP1 LMNA knockout whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: NIH/3T3 whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [4C11] (ab238303), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [4C11] (ab238303)

    IHC image of Lamin A + C staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BONDTM system using the standard protocol F.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab238303, 0.1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [4C11] (ab238303), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [4C11] (ab238303)

    ab238303 staining Lamin A+C in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab238303 at 1 μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control), at 1/1000 dilution. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)) at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150084 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594)) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

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