JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB227176

Anti-Lamin A + Lamin C antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Polyclonal Lamin-A/C antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LMNA.

View Alternative Names

LMN1, LMNA, Prelamin-A/C

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (AB227176)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (AB227176)

Paraffin-embedded human Mahlarvu xenograft tissue stained for Lamin A + C using ab227176 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody (AB227176)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody (AB227176)

Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Lamin A + C (green) using ab227176 at 1/500 dilution in ICC/IF.

Counterstain : Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (red) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (AB227176)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (AB227176)

Paraffin-embedded human breast carcinoma tissue stained for Lamin A + C using ab227176 at 1/250 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-Lamin A + Lamin C antibody (AB227176)
  • IP

Supplier Data

Immunoprecipitation - Anti-Lamin A + Lamin C antibody (AB227176)

Lamin A + C was immunoprecipitated from HeLa whole cell extract with 4 μg of ab227176. Western blot was performed from the immunoprecipitate using ab227176 at 1/500 dilution. Anti-rabbit IgG was used as secondary antibody.

Lane 1 : HeLa whole cell extract 50 μg (Input).

Lane 2 : Preimmune Rabbit IgG instead of ab227176 in HeLa whole cell extract.

Lane 3 : ab227176 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-Lamin A + Lamin C antibody (ab227176)

Predicted band size: 74 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (AB227176)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (AB227176)

Paraffin-embedded mouse C2C12 xenograft tissue stained for Lamin A + C using ab227176 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (AB227176)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody (AB227176)

Paraffin-embedded rat RT2 xenograft tissue stained for Lamin A + C using ab227176 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-Lamin A + Lamin C antibody (AB227176)
  • WB

Supplier Data

Western blot - Anti-Lamin A + Lamin C antibody (AB227176)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Lamin A + Lamin C antibody (ab227176) at 1/2000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 74 kDa

false

Western blot - Anti-Lamin A + Lamin C antibody (AB227176)
  • WB

Supplier Data

Western blot - Anti-Lamin A + Lamin C antibody (AB227176)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Lamin A + Lamin C antibody (ab227176) at 1/1000 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human LMNA. The exact immunogen used to generate this antibody is proprietary information.

P02545

style
left-column

Alternative Products

Primary Antibodies

AB108922

Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - Nuclear Envelope Marker (AB108922)

  • 5
  • 6
Primary Antibodies

AB185014

Alexa Fluor® 488 Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB185014)

  • 0
  • 0
Primary Antibodies

AB169532

Anti-Lamin A + Lamin C antibody [EPR4519(2)]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

  • 5
  • 1
Primary Antibodies

AB108595

Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB108595)

  • 5
  • 9
Primary Antibodies

AB210433

PE Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - PE Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB210433)

  • 0
  • 0
Primary Antibodies

AB193904

HRP Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB193904)

  • 0
  • 0
Primary Antibodies

AB133256

Anti-Lamin A + Lamin C antibody [EP4520-16]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EP4520-16] (AB133256)

  • 5
  • 5
Primary Antibodies

AB133269

Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (AB133269)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB133741

Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)

  • 4
  • 15
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB151735

Anti-Lamin B2 antibody [EPR9701(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamin A and Lamin C also known as lamin A/C are proteins encoded by the LMNA gene. These proteins are key components of the nuclear envelope where they provide structural support and maintain the shape of the nucleus. The lamin A/C molecule has a molecular weight of approximately 60-70 kDa. Expression of lamin A and lamin C occurs predominantly in differentiated cells where these proteins integrate into the nuclear lamina alongside other lamin molecules like lamin B. Lamin A alone sometimes referred to by designations like 4C11 plays a significant role in mechanical support at a molecular level.
Biological function summary

Lamin A/C proteins play a role in maintaining nuclear stability chromosome organization and gene regulation. They are part of a complex network within the nuclear lamina that includes interactions with proteins and DNA. Lamin A with a molecular weight distinct from other lamins participates in assembling this supportive matrix and contributes to DNA maintenance and repair processes. Their interaction with chromatin and gene expression regulation emphasizes their influence on important cellular functions.

Pathways

Lamin A/C proteins engage in the mechanosensory signaling and DNA damage response pathways. They interact with pathways involving the nuclear envelope structure and have connections to proteins like emerin and nuclear actin. Lamin A's role in these pathways supports its involvement in responding to mechanical stress and preserving genomic integrity highlighting its integration with these cellular processes.

Mutations in lamin A/C are linked to disorders such as Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. These conditions highlight the importance of lamin A/C in cellular stability and nuclear integrity. Proteins such as emerin often relate to lamin A/C in these diseases as disruptions to their interactions can lead to compromised nuclear function and disease phenotypes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Lamin-A/C. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed : 10080180, PubMed : 10580070, PubMed : 10587585, PubMed : 10814726, PubMed : 11799477, PubMed : 12075506, PubMed : 12927431, PubMed : 15317753, PubMed : 18551513, PubMed : 18611980, PubMed : 2188730, PubMed : 22431096, PubMed : 2344612, PubMed : 23666920, PubMed : 24741066, PubMed : 31434876, PubMed : 31548606, PubMed : 37788673, PubMed : 37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed : 10080180, PubMed : 10580070, PubMed : 10587585, PubMed : 10814726, PubMed : 11799477, PubMed : 12075506, PubMed : 12927431, PubMed : 15317753, PubMed : 18551513, PubMed : 18611980, PubMed : 22431096, PubMed : 23666920, PubMed : 24741066, PubMed : 31548606, PubMed : 37788673, PubMed : 37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed : 23990565, PubMed : 25127216). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed : 2188730, PubMed : 2344612). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed : 10080180, PubMed : 10580070, PubMed : 10587585, PubMed : 10814726, PubMed : 11799477, PubMed : 12075506, PubMed : 12927431, PubMed : 15317753, PubMed : 18551513, PubMed : 18611980, PubMed : 22431096, PubMed : 23666920, PubMed : 31548606). Also involved in DNA repair : recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed : 31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed : 10080180, PubMed : 10814726, PubMed : 11799477, PubMed : 18551513, PubMed : 22431096). Required for osteoblastogenesis and bone formation (PubMed : 12075506, PubMed : 15317753, PubMed : 18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed : 10587585). Required for cardiac homeostasis (PubMed : 10580070, PubMed : 12927431, PubMed : 18611980, PubMed : 23666920).. Prelamin-A/C. Prelamin-A/C can accelerate smooth muscle cell senescence (PubMed : 20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed : 20458013).
See full target information LMNA
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14259 PubMed38961628

2024

Cardiac and skeletal muscle manifestations in the G608G mouse model of Hutchinson-Gilford progeria syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yeojin Hong,Alice Rannou,Nancy Manriquez,Jack Antich,Weixin Liu,Mario Fournier,Ariel Omidfar,Russell G Rogers

Molecular biology of the cell 34:br13 PubMed37342871

2023

Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina.

Applications

Unspecified application

Species

Unspecified reactive species

Elina Mäntylä,Toni Montonen,Lucio Azzari,Salla Mattola,Markus Hannula,Maija Vihinen-Ranta,Jari Hyttinen,Minnamari Vippola,Alessandro Foi,Soile Nymark,Teemu O Ihalainen

Nature methods 20:218-228 PubMed36690742

2023

Screening cell-cell communication in spatial transcriptomics via collective optimal transport.

Applications

Unspecified application

Species

Unspecified reactive species

Zixuan Cang,Yanxiang Zhao,Axel A Almet,Adam Stabell,Raul Ramos,Maksim V Plikus,Scott X Atwood,Qing Nie

Molecular medicine reports 24: PubMed34558653

2021

Ginsenoside Rk1 protects human melanocytes from HO‑induced oxidative injury via regulation of the PI3K/AKT/Nrf2/HO‑1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xiong,Jianing Yang,Kai Yan,Jing Guo

Molecular medicine (Cambridge, Mass.) 25:17 PubMed31060494

2019

Silencing of SAA1 inhibits palmitate- or high-fat diet induced insulin resistance through suppression of the NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wang,Feng Cao,Yang Wang,Gang Yu,Ben-Li Jia
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com