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Anti-Lamin A + Lamin C antibody [EPR4100] ab108595 is a rabbit monoclonal antibody that is used in Lamin A + Lamin C western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with LMNA knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR4100 is cited in over 150 publications


Images

Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB108595), expandable thumbnail
  • Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB108595), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB108595), expandable thumbnail
  • Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB108595), expandable thumbnail
  • Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB108595), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/150

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Lamin-A/CLamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:2344612, PubMed:2188730, PubMed:24741066, PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:24741066, PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:25127216, PubMed:23990565). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2344612, PubMed:2188730). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:23666920, PubMed:18611980).Prelamin-A/CPrelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).

Alternative names

Prelamin-A/C, LMNA, LMN1

Recommended products

Anti-Lamin A + Lamin C antibody [EPR4100] ab108595 is a rabbit monoclonal antibody that is used in Lamin A + Lamin C western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with LMNA knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR4100 is cited in over 150 publications

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4100

Purification technique

Affinity purification Protein A

Specificity

The antibody recognizes full length Lamin A/C and the cleaved large unit.

We have data to indicate that this antibody gives non-specific staining in IHC with mouse tissues. Based on this we believe the antibody is not suitable for use with mouse samples, as there will be non-specific staining.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Lamin A and Lamin C also known as lamin A/C are proteins encoded by the LMNA gene. These proteins are key components of the nuclear envelope where they provide structural support and maintain the shape of the nucleus. The lamin A/C molecule has a molecular weight of approximately 60-70 kDa. Expression of lamin A and lamin C occurs predominantly in differentiated cells where these proteins integrate into the nuclear lamina alongside other lamin molecules like lamin B. Lamin A alone sometimes referred to by designations like 4C11 plays a significant role in mechanical support at a molecular level.

Biological function summary

Lamin A/C proteins play a role in maintaining nuclear stability chromosome organization and gene regulation. They are part of a complex network within the nuclear lamina that includes interactions with proteins and DNA. Lamin A with a molecular weight distinct from other lamins participates in assembling this supportive matrix and contributes to DNA maintenance and repair processes. Their interaction with chromatin and gene expression regulation emphasizes their influence on important cellular functions.

Pathways

Lamin A/C proteins engage in the mechanosensory signaling and DNA damage response pathways. They interact with pathways involving the nuclear envelope structure and have connections to proteins like emerin and nuclear actin. Lamin A's role in these pathways supports its involvement in responding to mechanical stress and preserving genomic integrity highlighting its integration with these cellular processes.

Associated diseases and disorders

Mutations in lamin A/C are linked to disorders such as Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. These conditions highlight the importance of lamin A/C in cellular stability and nuclear integrity. Proteins such as emerin often relate to lamin A/C in these diseases as disruptions to their interactions can lead to compromised nuclear function and disease phenotypes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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19 product images

  • Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    ab108595 was shown to specifically react with Lamin A + C (LMNA) in wild type HAP1 cells. No band was observed when Lamin A + C (LMNA) knockout samples were used. Wild-type and Lamin A + C (LMNA) knockout samples were subjected to SDS-PAGE. The membrane was blocked for an hour using 5% milk before ab108595 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/10000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595) at 1/10000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: Lamin A/C knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 74 kDa

    Observed band size: 64 kDa, 76 kDa

  • Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595) at 1/100000 dilution

    All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 74 kDa

    Observed band size: 63 kDa, 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail
    Kanemura et al PLoS One. 2014 Jan 14;9(1):e85336. doi: 10.1371/journal.pone.0085336. eCollection 2014. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Histological analyses of hiPSCs (Human induced pluripotent stem cell) transplanted into the subretinal space of nude rats.

    Eye balls were excised from a nude rat 7 weeks after subretinal transplantation with 1×104 hiPSCs. Transplanted tissues were fixed with 4% paraformaldehyde. Paraffin embedded tissue sections were stained with haematoxylin/eosin. Then, the paraffin sections were deparaffinized with xylene and sequential 100%, 95%, 80%, 70% ethanol treatments for 5 min each. The sections were treated with 10 mM citric acid (pH 6) at 95°C for 50 min followed by permeation with 0.4% Triton-X in PBS at room temperature for 30 min.

    The deparaffinized sections were stained with ab108595 (Panel M), Hoechst 33258 (Panel N).

  • Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595) at 1/100000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

    Lane 2: HaCaT (Human keratinocyte cell line) cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 74 kDa

    Observed band size: 63 kDa, 74 kDa

  • Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    All lanes: Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595) at 1/10000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates at 10 µg

    Lane 2: SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysates at 10 µg

    Predicted band size: 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Lamin A + C (green) with purified ab108595 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: Primary antibody (1/500) and secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Immunocytochemsitry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Lamin A + C with unpurified ab108595 at 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labeling Lamin A + C with purified ab108595 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

    Negative control using PBS instead of primary antibody (inset).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling Lamin A + C with ab108595 at 1/250 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labeling Lamin A + C with ab108595 at 1/250 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunoprecipitation - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    ab108595 (purified) at 1/30 immunoprecipitating Lamin A + C in HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Predicted band size: 74 kDa

    Observed band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling Lamin A + C with ab108595.

    Green - Lamin A + C.

    Red - PI.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling Lamin A + C with ab108595.

    Green - Lamin A + C.

    Red - PI.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urothelial carcinoma tissue labeling Lamin A + C with ab108595.

    Green - Lamin A + C.

    Red - PI.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic carcinoma tissue labeling Lamin A + C with ab108595.

    Green - Lamin A + C.

    Red - PI.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Intracellular Flow Cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Lamin A + C with purified ab108595 at 1/110 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

    Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.

  • Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with unpurified ab108595 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108595, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human Lamin A and Lamin C.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Lamin A + Lamin C antibody - (ab108595) staining at 64-76KDa dilution.

    In Western blot, Anti-Flag tag staining at 1/10000 dilution.

    In Western blot, (Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Progerin antibody [EPR28694-72] (Anti-Progerin antibody [EPR28694-72] ab320642) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a DDDDK-tag, whole cell lysate at 20 µg

    Lane 2: HEK-293T cells transfected with a human progerin expression vector containing a Flag-tag, whole cell lysate at 20 µg

    Lane 3: HEK-293T cells transfected with a human Lamin C expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 4: HEK-293T cells transfected with a human Lamin A expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 74 kDa, 36 kDa, 64-76 kDa

    Exposure time: 10s

  • Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Western blot analysis of HEK-293T cells transfected with a human progerin expression vector containing a Flag-tag whole cell lysate 2 ug mixed with HeLa whole cell lysate 20 ug. The membrane was cut into strips and each strip was incubated separately with the following antibodies: Lane 1: anti Lamin A + Lamin C antibody ab108595 (1:1000), Lane 2: anti Flag-tag antibody (1:10000), Lane 3: anti-progerin Anti-Progerin antibody [EPR28694-72] ab320642 (1:1000).

    The anti-progerin antibody Anti-Progerin antibody [EPR28694-72] ab320642 specifically detects human progerin.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12714972, PMID: 33408413, PMID: 34694158).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Lamin A + Lamin C antibody - (ab108595) staining at 64-76KDa dilution.

    In Western blot, Anti-Flag tag staining at 1/10000 dilution.

    All lanes: Western blot - Anti-Progerin antibody [EPR28694-72] (Anti-Progerin antibody [EPR28694-72] ab320642) at 1/1000 dilution

    All lanes: HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human progerin expression vector containing a Flag-tag whole cell lysate 2 μg mixed with HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 74 kDa, 64-76 kDa

    Exposure time: 81s

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