Anti-Lamin A + Lamin C antibody [EPR4519(2)]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

ab169532 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Lamin A + C with ab169532 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

ab169532 showing +ve staining in Human normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

ab169532 showing +ve staining in Human normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

ab169532 showing +ve staining in Human normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling Lamin A + C with ab169532 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

Lanes 1 - 4 : Merged signal (red and green). Green - ab169532 observed at 70-75 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab169532 was shown to react with LMNA in wild-type HAP1 cells in Western blot. Loss of signal was observed when LMNA knockout sample was used. HAP1 wild-type and LMNA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab169532 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532) at 1/5000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

LMNA knockout HAP1 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Predicted band size: 74 kDa

Observed band size: 70-75 kDa

false

• WB

Lab

Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

Blocking and Diluting buffer 5% NFDM /TBST

65-74 kDa : full length;
45-50 kDa : cleavage forms

All lanes:

Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

Predicted band size: 74 kDa

Observed band size: 74 kDa

false

Exposure time: 30s

• WB

Unknown

Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

All lanes:

Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532) at 1/10000 dilution

Lane 1:

PC-12 cell lysate at 10 µg

Lane 2:

THP-1 cell lysate at 10 µg

Lane 3:

HeLa + Staurosporine cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 74 kDa

false

• WB

CiteAb

Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (AB169532)

Lamin A + Lamin C western blot using anti-Lamin A + Lamin C antibody [EPR4519(2)] ab169532. Publication image and figure legend from Zhang, Y., Li, D., et al., 2018, Cell Death Dis, PubMed 29970890.

ab169532 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab169532 please see the product overview.

Identification of the non-cytotoxic concentration of ZLDI-8 in MHCC97-H cells.a Molecular docking for the interaction between ZLDI-8 and ADAM-17. b MHCC97-H cells were treated with indicated concentration of ZLDI-8, and the inhibition rates were calculated. c The effect of 1 μmol/L or 3 μmol/L ZLDI-8 was examined at each time point. d MHCC97-H, which were treated with indicated concentration of ZLDI-8, were fractionated into cytoplasmic and nuclear fractions. The fractions were detected with anti-NICD antibody and ADAM-17 antibodies. β-actin was chosen as a cytoplasmic marker and Lamin A/C was the nuclear indicator. e, f MHCC97-H cells, which were treated with 1 μmol/L ZLDI-8, were examined to detect the protein level of anti-apoptosis/pro-survival (e) or EMT (f) related regulators

false