Skip to main content

Mouse Monoclonal Lamin-A/C antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Human, African green monkey samples. Cited in 45 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LMNA.


Images

Flow Cytometry - Anti-Lamin A + Lamin C antibody [JOL2] (AB40567), expandable thumbnail
  • Western blot - Anti-Lamin A + Lamin C antibody [JOL2] (AB40567), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [JOL2] (AB40567), expandable thumbnail
  • Western blot - Anti-Lamin A + Lamin C antibody [JOL2] (AB40567), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [JOL2] (AB40567), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: Fetal calf serum, 3.16% Tris HCl

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human LMNA. The exact immunogen used to generate this antibody is proprietary information. Database link P02545

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Tested
Tested
African green monkey
Expected
Expected
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform antigen retrieval with 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes.

Expected
Expected

Species

African green monkey

Dilution info

-

Notes

Perform antigen retrieval with 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes.

Tested
Tested

Species

Human

Dilution info

1/1.00000 - 1/10.00000

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

African green monkey

Dilution info

1/1.00000 - 1/10.00000

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/1.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

African green monkey

Dilution info

1/1.00000 - 1/200.00000

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Lamin-A/CLamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:2344612, PubMed:2188730, PubMed:24741066, PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:24741066, PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:25127216, PubMed:23990565). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2344612, PubMed:2188730). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:23666920, PubMed:18611980).Prelamin-A/CPrelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).

Alternative names

Prelamin-A/C, LMNA, LMN1

Recommended products

Mouse Monoclonal Lamin-A/C antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Human, African green monkey samples. Cited in 45 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LMNA.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human LMNA. The exact immunogen used to generate this antibody is proprietary information. Database link P02545
Clone number

JOL2

Purity

Tissue culture supernatant

Specificity

This antibody reacts with both recombinant and native Lamin A and C in humans.

Epitope

This product has been shown to bind to an epitope between amino acids 464-572.

Concentration
Loading...
Purification notes

This product is unpurified.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Lamin A and Lamin C also known as lamin A/C are proteins encoded by the LMNA gene. These proteins are key components of the nuclear envelope where they provide structural support and maintain the shape of the nucleus. The lamin A/C molecule has a molecular weight of approximately 60-70 kDa. Expression of lamin A and lamin C occurs predominantly in differentiated cells where these proteins integrate into the nuclear lamina alongside other lamin molecules like lamin B. Lamin A alone sometimes referred to by designations like 4C11 plays a significant role in mechanical support at a molecular level.

Biological function summary

Lamin A/C proteins play a role in maintaining nuclear stability chromosome organization and gene regulation. They are part of a complex network within the nuclear lamina that includes interactions with proteins and DNA. Lamin A with a molecular weight distinct from other lamins participates in assembling this supportive matrix and contributes to DNA maintenance and repair processes. Their interaction with chromatin and gene expression regulation emphasizes their influence on important cellular functions.

Pathways

Lamin A/C proteins engage in the mechanosensory signaling and DNA damage response pathways. They interact with pathways involving the nuclear envelope structure and have connections to proteins like emerin and nuclear actin. Lamin A's role in these pathways supports its involvement in responding to mechanical stress and preserving genomic integrity highlighting its integration with these cellular processes.

Associated diseases and disorders

Mutations in lamin A/C are linked to disorders such as Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. These conditions highlight the importance of lamin A/C in cellular stability and nuclear integrity. Proteins such as emerin often relate to lamin A/C in these diseases as disruptions to their interactions can lead to compromised nuclear function and disease phenotypes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567), expandable thumbnail

    Flow Cytometry - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567)

    Overlay histogram showing HeLa cells stained with ab40567 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40567, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567)

    Lanes 1 - 4: Merged signal (red and green). Green - ab40567 observed at 74 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    ab40567 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in LMNA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LMNA knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. ab40567 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/200 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567) at 1/200 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: LMNA knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: HepG2 whole cell lysate at 20 µg

    Predicted band size: 74 kDa

    Observed band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling Lamin A + C with ab40567.

  • Western blot - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567)

    All lanes: Western blot - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567) at 1/200 dilution

    All lanes: HeLa whole cell lysate (10ug)

    Secondary

    All lanes: HRP conjugated goat anti-mouse antibody

    Developed using the ECL technique.

    Predicted band size: 74 kDa

    Observed band size: 64 kDa, 73 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [JOL2] (ab40567)

    Formalin-fixed, paraffin-embedded human skin tissue stained for Lamin A + C using ab40567 in immunohistochemical analysis. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes and then let to stand at room temperature in a buffer for a further 20 minutes. They were then rinsed in 1X TBS with Tween (TBST) for 1 minute at room temperature.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com