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Rabbit Recombinant Monoclonal Lamin-A/C phospho S22 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human LMNA phospho S22.

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Images

Flow Cytometry - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (AB278643), expandable thumbnail
  • Flow Cytometry - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (AB278643), expandable thumbnail
  • Western blot - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (AB278643), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.88% PBS, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human LMNA phospho S22. The exact immunogen used to generate this antibody is proprietary information. Database link P02545

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Tested
Mouse
Expected
Tested

Tested
Tested

Species
Human
Dilution info
0.00100-1.00000 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.01 µg/mL
Notes

-

Species
Human
Dilution info
0.01 µg/mL
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Lamin-A/C. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:2188730, PubMed:22431096, PubMed:2344612, PubMed:23666920, PubMed:24741066, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:24741066, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:23990565, PubMed:25127216). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2188730, PubMed:2344612). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920). Prelamin-A/C. Prelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).

Alternative names

LMN1, LMNA, Prelamin-A/C

Recommended products

Rabbit Recombinant Monoclonal Lamin-A/C phospho S22 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human LMNA phospho S22.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human LMNA phospho S22. The exact immunogen used to generate this antibody is proprietary information. Database link P02545
Clone number
LaminACS22-CF12
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Lamin A and Lamin C also known as lamin A/C are proteins encoded by the LMNA gene. These proteins are key components of the nuclear envelope where they provide structural support and maintain the shape of the nucleus. The lamin A/C molecule has a molecular weight of approximately 60-70 kDa. Expression of lamin A and lamin C occurs predominantly in differentiated cells where these proteins integrate into the nuclear lamina alongside other lamin molecules like lamin B. Lamin A alone sometimes referred to by designations like 4C11 plays a significant role in mechanical support at a molecular level.

Biological function summary

Lamin A/C proteins play a role in maintaining nuclear stability chromosome organization and gene regulation. They are part of a complex network within the nuclear lamina that includes interactions with proteins and DNA. Lamin A with a molecular weight distinct from other lamins participates in assembling this supportive matrix and contributes to DNA maintenance and repair processes. Their interaction with chromatin and gene expression regulation emphasizes their influence on important cellular functions.

Pathways

Lamin A/C proteins engage in the mechanosensory signaling and DNA damage response pathways. They interact with pathways involving the nuclear envelope structure and have connections to proteins like emerin and nuclear actin. Lamin A's role in these pathways supports its involvement in responding to mechanical stress and preserving genomic integrity highlighting its integration with these cellular processes.

Associated diseases and disorders

Mutations in lamin A/C are linked to disorders such as Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. These conditions highlight the importance of lamin A/C in cellular stability and nuclear integrity. Proteins such as emerin often relate to lamin A/C in these diseases as disruptions to their interactions can lead to compromised nuclear function and disease phenotypes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (ab278643), expandable thumbnail

    Flow Cytometry - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (ab278643)

    Flow cytometric analysis of HeLa cells, secondary antibody only negative control (blue), or untreated (grey) or treated with nocodazole (orange) using 0.01 ug/mL isotype control, or untreated (red) or treated (green) using ab278643 at 0.01 µg/mL.

  • Flow Cytometry - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (ab278643), expandable thumbnail

    Flow Cytometry - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (ab278643)

    Flow cytometric analysis of NIH 3T3 cells, secondary antibody only negative control (blue), or untreated (red) or treated with nocodazole (green) using ab278643 at 0.05 µg/mL.

  • Western blot - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (ab278643), expandable thumbnail

    Western blot - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (ab278643)

    All lanes: Western blot - Anti-Lamin A + Lamin C (phospho S22) antibody [LaminACS22-CF12] (ab278643) at 0.01 µg/mL

    Lane 1: HeLa cell extract treated with nocodazole

    Lane 2: HeLa cell extract untreated

    Predicted band size: 74 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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