AB122919

Anti-Lamin B Receptor/LBR antibody

Name: Anti-Lamin B Receptor/LBR antibody (ab122919) | Abcam
Brand: Abcam Limited
SKU: AB122919
Rating: 3 (2 reviews)

(2 Reviews)

(8 Publications)

Rabbit Polyclonal Lamin B Receptor/LBR antibody. Suitable for IP, ICC/IF and reacts with Human samples. Cited in 8 publications.

View Alternative Names

Delta(14)-sterol reductase LBR, Delta-14-SR, 3-beta-hydroxysterol Delta (14)-reductase, C-14 sterol reductase, Integral nuclear envelope inner membrane protein, LMN2R, Lamin-B receptor, Sterol C14-reductase, C14SR, LBR

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody (AB122919)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody (AB122919)

Indirect immuno-fluorescence staining of HeLa cells using ab122919.

Unknown

Immunoprecipitation - Anti-Lamin B Receptor/LBR antibody (AB122919)

Identification of Lamin B Receptor/LBR in crude extract of HeLa cells by immuno-precipitation using ab122919, followed by Western blotting.

All lanes:

Immunoprecipitation - Anti-Lamin B Receptor/LBR antibody (ab122919)

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab122919 is affinity purified using recombinant immunogen.

Storage buffer

pH: 6 - 8.5 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Lamin B Receptor (LBR) also known as L. B. R. L B R or lamin B is a protein located in the inner nuclear membrane. This protein has a molecular mass of approximately 70 kDa. It acts as a receptor for lamin proteins specifically lamin B. The LBR mediates the attachment of heterochromatin and lamins to the inner nuclear membrane playing a structural role in the maintenance of nuclear architecture. It is widely expressed in various tissues notably in cells involved in rapidly proliferating tissues.

Biological function summary

The LBR protein serves several critical functions beyond its mechanical duties. It participates in the regulation of chromatin organization and gene expression owing to its interaction with chromatin-related proteins. LBR is part of the nuclear envelope lattice and forms complexes with lamin proteins and heterochromatin. This association supports its role in anchoring chromatin at the nuclear periphery modulating processes such as DNA replication and transcription.

Pathways

The LBR plays pivotal roles in nuclear envelope assembly and chromatin organization. It holds significance in the cholesterol biosynthesis pathway where it metabolizes sterol intermediates to cholesterol. This function relates LBR to other proteins including lamin A/C which collaborate in maintaining nuclear shape and function. Together they orchestrate various cellular processes important for cell cycle progression and differentiation.

Alterations in LBR function have links to conditions such as Pelger-Huët anomaly and Greenberg skeletal dysplasia. Pelger-Huët anomaly manifests as a benign hematologic condition with defective nuclear shape in neutrophils while Greenberg dysplasia is characterized by skeletal abnormalities. In these contexts LBR connects to lamin B and other nuclear envelope proteins that contribute to the pathophysiological features observed in these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis (PubMed : 12618959, PubMed : 16784888, PubMed : 21327084, PubMed : 27336722, PubMed : 9630650). Plays a critical role in myeloid cell cholesterol biosynthesis which is essential to both myeloid cell growth and functional maturation (By similarity). Mediates the activation of NADPH oxidases, perhaps by maintaining critical levels of cholesterol required for membrane lipid raft formation during neutrophil differentiation (By similarity). Anchors the lamina and the heterochromatin to the inner nuclear membrane (PubMed : 10828963).

See full target information LBR

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 34:ar105 PubMed37556227

2023

A dedicated cytoplasmic container collects extrachromosomal DNA away from the mammalian nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Schenkel,Xuan Wang,Nhung Le,Michael Burger,Ruth Kroschewski

Nature communications 12:3176 PubMed34039995

2021

The SUN1-SPDYA interaction plays an essential role in meiosis prophase I.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Chen,Yan Wang,Juan Chen,Wu Zuo,Yong Fan,Sijia Huang,Yongmei Liu,Guangming Chen,Qing Li,Jinsong Li,Jian Wu,Qian Bian,Chenhui Huang,Ming Lei

Molecular cell 81:2216-2230.e10 PubMed33848455

2021

Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance.

Applications

Unspecified application

Species

Unspecified reactive species

Ruben Schep,Eva K Brinkman,Christ Leemans,Xabier Vergara,Robin H van der Weide,Ben Morris,Tom van Schaik,Stefano G Manzo,Daniel Peric-Hupkes,Jeroen van den Berg,Roderick L Beijersbergen,René H Medema,Bas van Steensel

Cancer management and research 12:7501-7514 PubMed32943917

2020

Exosomal CircGDI2 Suppresses Oral Squamous Cell Carcinoma Progression Through the Regulation of MiR-424-5p/SCAI Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Kaiqi Tang,Lizhu Chen,Meng Du,Zhi Qu

Molecular medicine reports 19:1222-1229 PubMed30535473

2018

Chrysin protects human osteoarthritis chondrocytes by inhibiting inflammatory mediator expression via HMGB1 suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Zhang,Weizhong Yu,Chongbo Huang,Qinghe Ding,Chizhang Liang,Le Wang,Zhiqi Hou,Zhiyong Zhang

Human molecular genetics 27:407-420 PubMed29186574

2017

TorsinA dysfunction causes persistent neuronal nuclear pore defects.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel S Pappas,Chun-Chi Liang,Sumin Kim,CheyAnne O Rivera,William T Dauer

Nature materials 15:1287-1296 PubMed27548707

2016

Transcription upregulation via force-induced direct stretching of chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Arash Tajik,Yuejin Zhang,Fuxiang Wei,Jian Sun,Qiong Jia,Wenwen Zhou,Rishi Singh,Nimish Khanna,Andrew S Belmont,Ning Wang

Nucleus (Austin, Tex.) 5:318-30 PubMed25482121

2014

Sequestration within nuclear chromocenters is not a requirement for silencing olfactory receptor transcription in a placode-derived cell line.

Applications

Species

Unspecified reactive species

Seda Kilinc,Diane T Meredith,Robert P Lane

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lamin B Receptor/LBR antibody

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody (AB122919)

Immunoprecipitation - Anti-Lamin B Receptor/LBR antibody (AB122919)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise