AB169306

Anti-Lamin B Receptor/LBR antibody

Name: Anti-Lamin B Receptor/LBR antibody (ab169306) | Abcam
Brand: Abcam Limited
SKU: AB169306

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(4 Publications)

Mouse Polyclonal Lamin B Receptor/LBR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LBR.

View Alternative Names

Delta(14)-sterol reductase LBR, Delta-14-SR, 3-beta-hydroxysterol Delta (14)-reductase, C-14 sterol reductase, Integral nuclear envelope inner membrane protein, LMN2R, Lamin-B receptor, Sterol C14-reductase, C14SR, LBR

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody (AB169306)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody (AB169306)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling Lamin B Receptor/LBR with ab169306 at 3μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody (AB169306)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody (AB169306)

Immunofluorescent analysis of HeLa cells labeling Lamin B Receptor/LBR with ab169306 at 10μg/ml.

Unknown

Western blot - Anti-Lamin B Receptor/LBR antibody (AB169306)

All lanes:

Western blot - Anti-Lamin B Receptor/LBR antibody (ab169306) at 1 µg/mL

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 71 kDa

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Western blot - Anti-Lamin B Receptor/LBR antibody (AB169306)

All lanes:

Western blot - Anti-Lamin B Receptor/LBR antibody (ab169306) at 1 µg/mL

Lane 1:

Lamin B Receptor/LBR transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 71 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LBR.

Q14739

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3 µg/mL", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Lamin B Receptor (LBR) also known as L. B. R. L B R or lamin B is a protein located in the inner nuclear membrane. This protein has a molecular mass of approximately 70 kDa. It acts as a receptor for lamin proteins specifically lamin B. The LBR mediates the attachment of heterochromatin and lamins to the inner nuclear membrane playing a structural role in the maintenance of nuclear architecture. It is widely expressed in various tissues notably in cells involved in rapidly proliferating tissues.

Biological function summary

The LBR protein serves several critical functions beyond its mechanical duties. It participates in the regulation of chromatin organization and gene expression owing to its interaction with chromatin-related proteins. LBR is part of the nuclear envelope lattice and forms complexes with lamin proteins and heterochromatin. This association supports its role in anchoring chromatin at the nuclear periphery modulating processes such as DNA replication and transcription.

Pathways

The LBR plays pivotal roles in nuclear envelope assembly and chromatin organization. It holds significance in the cholesterol biosynthesis pathway where it metabolizes sterol intermediates to cholesterol. This function relates LBR to other proteins including lamin A/C which collaborate in maintaining nuclear shape and function. Together they orchestrate various cellular processes important for cell cycle progression and differentiation.

Alterations in LBR function have links to conditions such as Pelger-Huët anomaly and Greenberg skeletal dysplasia. Pelger-Huët anomaly manifests as a benign hematologic condition with defective nuclear shape in neutrophils while Greenberg dysplasia is characterized by skeletal abnormalities. In these contexts LBR connects to lamin B and other nuclear envelope proteins that contribute to the pathophysiological features observed in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis (PubMed : 12618959, PubMed : 16784888, PubMed : 21327084, PubMed : 27336722, PubMed : 9630650). Plays a critical role in myeloid cell cholesterol biosynthesis which is essential to both myeloid cell growth and functional maturation (By similarity). Mediates the activation of NADPH oxidases, perhaps by maintaining critical levels of cholesterol required for membrane lipid raft formation during neutrophil differentiation (By similarity). Anchors the lamina and the heterochromatin to the inner nuclear membrane (PubMed : 10828963).

See full target information LBR

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 44:3025-3036 PubMed40550847

2025

Vimentin intermediate filaments orchestrate DNA nonhomologous end joining repair and lipolysis after DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Wang,Mengtao Rong,Liang Zhang,Abhishikt D Solomon,Wenli Gui,Juan Li,Renqing Wang,Jiajing Wu,Ling Wang,Xingyuan Yang,Aimin Peng

Frontiers in pharmacology 11:1146 PubMed32848758

2020

7-Hydroxycoumarin Attenuates Colistin-Induced Kidney Injury in Mice Through the Decreased Level of Histone Deacetylase 1 and the Activation of Nrf2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Muhammad Ishfaq,Qianqian Fan,Chunli Chen,Jichang Li

FEBS letters 593:2556-2565 PubMed31254363

2019

Protective effect of histone methyltransferase NSD3 on ISO-induced cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Chen,Dongdong Jian,Linwei Zhao,Xiaobiao Zang,Weifeng Song,Jifang Ma,Zhen Jia,Xianqing Wang,Chuanyu Gao

Nucleic acids research 43:3114-27 PubMed25753664

2015

Insulin-response epigenetic activation of Egr-1 and JunB genes at the nuclear periphery by A-type lamin-associated pY19-Caveolin-2 in the inner nuclear membrane.

Applications

Unspecified application

Species

Rat

Kyuho Jeong,Hayeong Kwon,Jaewoong Lee,Donghwan Jang,Yunbae Pak

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Lamin B Receptor/LBR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody (AB169306)

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody (AB169306)

Western blot - Anti-Lamin B Receptor/LBR antibody (AB169306)

Western blot - Anti-Lamin B Receptor/LBR antibody (AB169306)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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