Skip to main content

Mouse Monoclonal Lamin B1 antibody. Nuclear Envelope marker. Suitable for Flow Cyt, ICC/IF, IHC-Fr and reacts with Human, Mouse, Pig samples. Cited in 45 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982), expandable thumbnail
  • Flow Cytometry - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (AB8982), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IFIHC-Fr
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Pig
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/200

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Pig

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

0.5 µg/mL

Notes

-

Species

Human

Dilution info

0.5 µg/mL

Notes

-

Expected
Expected

Species

Pig

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Pig, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.

Alternative names

Lamin-B1

Recommended products

Mouse Monoclonal Lamin B1 antibody. Nuclear Envelope marker. Suitable for Flow Cyt, ICC/IF, IHC-Fr and reacts with Human, Mouse, Pig samples. Cited in 45 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
Clone number

119D5-F1

Purification technique

Affinity purification Protein G

Specificity

Reacts with an epitope located C-terminal of residue 231 in lamin B1.
Reacts against lamin B1, does not cross react with lamin B2.

Lamins do not appear to be universally distributed among different cell and tissue types. ab8982 has been shown to react with HeLa and 3T3 cells in immunocytochemistry. Other cell/tissue types have not been tested.

Epitope

C-terminal to residue 231.

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Lamin B1 also known as LMNB1 is a protein that plays an important role in the structure of the nuclear lamina a dense fibrillar network inside the nucleus of cells. This protein belongs to the lamin family and has an approximate molecular weight of 66 kDa. Lamin B1 is expressed in nearly all types of cells acting as a structural component by interacting with chromatin and anchoring the nuclear envelope. It contributes to regulating nuclear stability and mechanical support which is essential for proper cell function.

Biological function summary

Lamin B1 serves many important functions by providing structural integrity and regulating gene expression through chromatin organization. Part of the nuclear lamina complex Lamin B1 interacts with other lamin proteins such as Lamin A and Lamin C forming a mesh-like structure that supports the nuclear envelope. This protein also affects cellular processes such as DNA replication RNA transcription and cell division indicating its broad impact on various cellular activities.

Pathways

Lamin B1 plays an important role in gene expression regulation and cell cycle control. It interacts with pathways involved in chromatin remodeling influencing the access of transcription factors to DNA which affects gene expression profiles. Lamin B1 associates with related proteins such as Lamin A/C and other nuclear matrix proteins to maintain cellular architecture stability and normal cell function. This organization is vital to ensure the accuracy of cellular processes and prevent anomalies during cell division.

Associated diseases and disorders

Mutations or altered expression of Lamin B1 have been associated with several pathological conditions. One noteworthy disorder is autosomal dominant adult-onset leukodystrophy where Lamin B1 expression levels are abnormal. This protein also connects to multiple sclerosis through its role in maintaining nuclear architecture. Additionally changes in Lamin B1 expression levels can influence pathways involving Lamin A/C and potentially lead to other laminopathies highlighting its influence in maintaining cellular health and contributing to disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    ab8982 staining Lamin B1 in wild-type HAP1 cells (top panel) and LMNB1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8982 at 0.5μg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982), expandable thumbnail

    Flow Cytometry - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Overlay histogram showing HeLa cells stained with ab8982 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (Normal Goat Serum ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8982, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Immunohistochemistry on frozen sections of human kidney showing nuclear lamina staining in epithelial and connective tissue cells.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Immunohistochemistry of MCF-7 cell culture showing nuclear lamina staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982), expandable thumbnail
    This image is courtesy of Marilena Ciciarello & Patrizia Lavia, University La Sapienza

    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Methanol fixed HeLa and 3T3 cells (NIH/3T3 whole cell lysate ab7179) were stained with ab8982 (1/50 and 1/20 respectively). The cells were fixed in 100% methanol for 6 minutes at -20°C. ab8982 clearly stains the nuclear envelope with very little background staining.

    A: HeLa cells + ab8982 (1/50) (green)
    B: HeLa cells counterstained with DAPI (blue)
    C. 3T3 cells + ab8982 (1/20) (green)
    D. 3T3 cells counterstained with DAPI (blue)

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Immunohistochemistry on frozen sections of swine liver showing nuclear lamina staining in hepatocytes.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com