Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Rabbit Monoclonal Lamin B1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 17 publications.

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Related conjugates and formulations

ab133741
Unconjugated
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker
ab194106
Conjugated
Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker
ab194109
Conjugated
HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control
ab194108
Conjugated
Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker
ab216723
Conjugated
Alexa Fluor® 594 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker

Images

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-P
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Tested	Tested
Rat	Expected	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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6 products for Alternative Product

4 products for Alternative Version

Target data

See full target information LMNB1

Function

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:28716252, PubMed:32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:28716252, PubMed:32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:28716252, PubMed:32910914).

Alternative names

LMN2, LMNB, LMNB1, Lamin-B1

Recommended products

Rabbit Monoclonal Lamin B1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 17 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR8985(B)
Purification technique: Affinity purification Protein A
Dissociation constant: 1.95 x 10^-10 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab220797 is the carrier-free version of Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Lamin B1 also known as LMNB1 is a protein that plays an important role in the structure of the nuclear lamina a dense fibrillar network inside the nucleus of cells. This protein belongs to the lamin family and has an approximate molecular weight of 66 kDa. Lamin B1 is expressed in nearly all types of cells acting as a structural component by interacting with chromatin and anchoring the nuclear envelope. It contributes to regulating nuclear stability and mechanical support which is essential for proper cell function.

Biological function summary

Lamin B1 serves many important functions by providing structural integrity and regulating gene expression through chromatin organization. Part of the nuclear lamina complex Lamin B1 interacts with other lamin proteins such as Lamin A and Lamin C forming a mesh-like structure that supports the nuclear envelope. This protein also affects cellular processes such as DNA replication RNA transcription and cell division indicating its broad impact on various cellular activities.

Pathways

Lamin B1 plays an important role in gene expression regulation and cell cycle control. It interacts with pathways involved in chromatin remodeling influencing the access of transcription factors to DNA which affects gene expression profiles. Lamin B1 associates with related proteins such as Lamin A/C and other nuclear matrix proteins to maintain cellular architecture stability and normal cell function. This organization is vital to ensure the accuracy of cellular processes and prevent anomalies during cell division.

Associated diseases and disorders

Mutations or altered expression of Lamin B1 have been associated with several pathological conditions. One noteworthy disorder is autosomal dominant adult-onset leukodystrophy where Lamin B1 expression levels are abnormal. This protein also connects to multiple sclerosis through its role in maintaining nuclear architecture. Additionally changes in Lamin B1 expression levels can influence pathways involving Lamin A/C and potentially lead to other laminopathies highlighting its influence in maintaining cellular health and contributing to disease development.

Product promise

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13 product images

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Clone EPR8985(B) (ab220797) has been successfully conjugated by Abcam. This image was generated using Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194108 for protocol details.
Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194108 staining Lamin B1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194108 at a 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (Human Burkitt's lymphoma cell line) cells labeling Lamin B1 with Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/200 dilution (green). Nuclear envelope staining on Ramos cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/200 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of the bladder tissue labeling Lamin B1 with purified Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 staining Lamin B1 in wild-type HAP1 cells (top panel) and Lamin B1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1μg/ml dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudo-color red) overnight at +4°C. The cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)) at 1/1000 dilution for 1 hour. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Lanes 1- 2: Merged signal (red and green). Green - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 observed at 66-70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 was shown to react with Lamin B1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human LMNB1 (Lamin B1) knockout HeLa cell line ab255404 (knockout cell lysate Human LMNB1 (Lamin B1) knockout HeLa cell lysate ab263825) was used. Wild-type HeLa and LMNB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: LMNB1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human LMNB1 (Lamin B1) knockout HeLa cell line (Human LMNB1 (Lamin B1) knockout HeLa cell line ab255404)
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 66-70 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Clone EPR8985(B) (ab220797) has been successfully conjugated by Abcam. This image was generated using Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194106 for protocol details.
Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194106 staining Lamin B1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194106 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 594, shown in red) at 1/250 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 was shown to specifically react with Lamin B1 in wild type HAP1 cells. No band was observed when knockout samples were used. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Lanes 1 and 3: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797) at 1/1000 dilution
Lanes 2 and 4: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lanes 2 and 4: Empty
Lane 3: LMNB1 knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 66 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunohistochemical staining of paraffin embedded Mouse Cerebral cortex with purified Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at a working dilution of 1/300. The secondary antibody used is a HRP polymer for rabbit IgG. Nuclear envelope staining on neuron cells of Cerebral cortex tissue is observed. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 (purified) at 1/20 immunoprecipitating Lamin B1 in Jurkat cells (Lane 1). For western blotting, Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 was used at 1/1000 dilution and an HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
All lanes: Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741)
Predicted band size: 66 kDa
Observed band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Lamin B1 with unpurified Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lamin B1 with unpurified Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
This data was developed using Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of formalin fixed paraffin embedded human colon labelling Lamin B1 with Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 anti-Lamin B1 antibody [EPR8985(B)] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

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Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free

Images

Publications

Key facts

Immunogen

Reactivity data

ICC/IF

TestedTested

ExpectedExpected

IP

TestedTested

ExpectedExpected

WB

TestedTested

IHC-P

TestedTested

ExpectedExpected

Associated Products

6 products for Alternative Product

4 products for Alternative Version

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

13 product images

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)

Downloads

Product protocols

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Expected