Rabbit Monoclonal Lamin B1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 17 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunocytochemistry-immunofluorescence-img70111.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunocytochemistry-immunofluorescence-img15024.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111560.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunocytochemistry-immunofluorescence-img119807.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunocytochemistry-immunofluorescence-img14907.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (AB220797)")
Publications
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- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22058622023AQP4 Aggravates Cognitive Impairment in Sepsis-Associated Encephalopathy through Inhibiting Na 1.6-Mediated Astrocyte Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan-Dan Zhu,Yue-Lin Huang,Song-Yu Guo,Na Li,Xue-Wei Yang,Ao-Ran Sui,Qiong Wu,Yue Zhang,Yue Kong,Qi-Fa Li,Ting Zhang,Wen-Fei Zheng,Ai-Ping Li,Jian Yu,Tong-Hui Ma,Shao LiPubMed 36922751
- STAR protocols 3:1010342022MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniëlle Krijgsman,Neeraj Sinha,Matthijs J D Baars,Stephanie van Dam,Mojtaba Amini,Yvonne VercoulenPubMed 34977680
- Stem cells international 2021:66443662021CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through Upregulating TAZ and Osteogenic Differentiation Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Li,Feifei Wang,Fusheng Dong,Zhiyong Zhang,Peng Song,Huizhen Chen,Jie WangPubMed 34394357
- Cellular and molecular gastroenterology and hepatology 12:395-4252021Helicobacter pylori-Induced Rev-erbα Fosters Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang-Yuan Mao,Yi-Pin Lv,Chuan-Jie Hao,Yong-Sheng Teng,Yu-Gang Liu,Ping Cheng,Shi-Ming Yang,Weisan Chen,Tao Liu,Quan-Ming Zou,Rui Xie,Jing-Yu Xu,Yuan ZhuangPubMed 33676046
- European journal of pharmacology 898:1739322021Sevoflurane protects the liver from ischemia-reperfusion injury by regulating Nrf2/HO-1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Ma,Baoyi Yang,Lu Yu,Yang Gao,Xiangmei Ye,Ying Liu,Zhengtian Li,Hulun Li,Enyou LiPubMed 33631180
- Nature 590:344-3502021Integrated spatial genomics reveals global architecture of single nuclei.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYodai Takei,Jina Yun,Shiwei Zheng,Noah Ollikainen,Nico Pierson,Jonathan White,Sheel Shah,Julian Thomassie,Shengbao Suo,Chee-Huat Linus Eng,Mitchell Guttman,Guo-Cheng Yuan,Long CaiPubMed 33505024
- Inflammation 44:1288-13012021Propofol Protects Against Hepatic Ischemia Reperfusion Injury via Inhibiting Bnip3-Mediated Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Ma,Ying Liu,Zhengtian Li,Lu Yu,Yang Gao,Xiangmei Ye,Baoyi Yang,Hulun Li,Jinghui ShiPubMed 33496895
- Neurochemical research 45:1839-18502020Docosahexaenoic Acid Protects Traumatic Brain Injury by Regulating NOX Generation via Nrf2 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhu et. al.PubMed 32676950
- Toxicology research 8:1028-10342019Phytochemicals protect L02 cells against hepatotoxicity induced by emodin the Nrf2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Yan et. al.PubMed 32153769
- Acta physiologica (Oxford, England) 223:e130372018Interferon-induced protein 35 inhibits endothelial cell proliferation, migration and re-endothelialization of injured arteries by inhibiting the nuclear factor-kappa B pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Jian,W Wang,X Zhou,Z Jia,J Wang,M Yang,W Zhao,Z Jiang,X Hu,J ZhuPubMed 29350881
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Tested |
| Rat | Expected | Expected | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
4 products for Alternative Version
Target data
Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Alternative names
Lamin-B1, LMNB, LMN2, LMNB1
Recommended products
Rabbit Monoclonal Lamin B1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 17 publications.
Alternative names
Lamin-B1, LMNB, LMN2, LMNB1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR8985(B)
- Purification technique
Affinity purification Protein A
- Dissociation constant
1.95 x 10-10 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab220797 is the carrier-free version of Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lamin B1 also known as LMNB1 is a protein that plays an important role in the structure of the nuclear lamina a dense fibrillar network inside the nucleus of cells. This protein belongs to the lamin family and has an approximate molecular weight of 66 kDa. Lamin B1 is expressed in nearly all types of cells acting as a structural component by interacting with chromatin and anchoring the nuclear envelope. It contributes to regulating nuclear stability and mechanical support which is essential for proper cell function.
- Biological function summary
Lamin B1 serves many important functions by providing structural integrity and regulating gene expression through chromatin organization. Part of the nuclear lamina complex Lamin B1 interacts with other lamin proteins such as Lamin A and Lamin C forming a mesh-like structure that supports the nuclear envelope. This protein also affects cellular processes such as DNA replication RNA transcription and cell division indicating its broad impact on various cellular activities.
- Pathways
Lamin B1 plays an important role in gene expression regulation and cell cycle control. It interacts with pathways involved in chromatin remodeling influencing the access of transcription factors to DNA which affects gene expression profiles. Lamin B1 associates with related proteins such as Lamin A/C and other nuclear matrix proteins to maintain cellular architecture stability and normal cell function. This organization is vital to ensure the accuracy of cellular processes and prevent anomalies during cell division.
- Associated diseases and disorders
Mutations or altered expression of Lamin B1 have been associated with several pathological conditions. One noteworthy disorder is autosomal dominant adult-onset leukodystrophy where Lamin B1 expression levels are abnormal. This protein also connects to multiple sclerosis through its role in maintaining nuclear architecture. Additionally changes in Lamin B1 expression levels can influence pathways involving Lamin A/C and potentially lead to other laminopathies highlighting its influence in maintaining cellular health and contributing to disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunocytochemistry-immunofluorescence-img70111.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Clone EPR8985(B) (ab220797) has been successfully conjugated by Abcam. This image was generated using Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194108 for protocol details.
Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194108 staining Lamin B1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194108 at a 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunocytochemistry-immunofluorescence-img15024.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (Human Burkitt's lymphoma cell line) cells labeling Lamin B1 with Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/200 dilution (green). Nuclear envelope staining on Ramos cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/200 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111560.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of the bladder tissue labeling Lamin B1 with purified Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunocytochemistry-immunofluorescence-img119807.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 staining Lamin B1 in wild-type HAP1 cells (top panel) and Lamin B1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1μg/ml dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudo-color red) overnight at +4°C. The cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)) at 1/1000 dilution for 1 hour. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunocytochemistry-immunofluorescence-img14907.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Clone EPR8985(B) (ab220797) has been successfully conjugated by Abcam. This image was generated using Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194106 for protocol details.
Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194106 staining Lamin B1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab194106 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/250 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--western-blot-img142367.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Lanes 1- 2: Merged signal (red and green). Green - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 observed at 66-70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 was shown to react with Lamin B1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human LMNB1 (Lamin B1) knockout HeLa cell line ab255404 (knockout cell lysate Human LMNB1 (Lamin B1) knockout HeLa cell lysate ab263825) was used. Wild-type HeLa and LMNB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: LMNB1 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 66-70 kDa
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--western-blot-img137995.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 was shown to specifically react with Lamin B1 in wild type HAP1 cells. No band was observed when knockout samples were used. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Lanes 1 and 3: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797) at 1/1000 dilution
Lanes 2 and 4: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lanes 2 and 4: Empty
Lane 3: LMNB1 knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 66 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115789.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunohistochemical staining of paraffin embedded Mouse Cerebral cortex with purified Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at a working dilution of 1/300. The secondary antibody used is a HRP polymer for rabbit IgG. Nuclear envelope staining on neuron cells of Cerebral cortex tissue is observed. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
![Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunoprecipitation-img96884.jpg "Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 (purified) at 1/20 immunoprecipitating Lamin B1 in Jurkat cells (Lane 1). For western blotting, Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 was used at 1/1000 dilution and an HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
All lanes: Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741)
Predicted band size: 66 kDa
Observed band size: 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70247.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Lamin B1 with unpurified Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--oi-rd-scanning-img98203.jpg "OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88855.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lamin B1 with unpurified Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab133741).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--western-blot-img142367.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--western-blot-img137995.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115789.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
![Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunoprecipitation-img96884.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70247.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
![OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--oi-rd-scanning-img98203.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-bsa-and-azide-free-ab220797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88855.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)")
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