Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker is a rabbit recombinant monoclonal antibody that is used to detect Lamin B1 in ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with LMNB1 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR8985(B) is cited in over 250 publications
- Sample sizes available
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunocytochemistry-immunofluorescence-img119807.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunocytochemistry-immunofluorescence-img15024.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111560.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)")
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img142367.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)")
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img139633.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)")
Publications
Filter by
- Epigenetics & chromatin 16:362023Nucleophosmin 1 cooperates with the methyltransferase DOT1L to preserve peri-nucleolar heterochromatin organization by regulating H3K27me3 levels and DNA repeats expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnalisa Izzo,Ipek Akol,Alejandro Villarreal,Shannon Lebel,Marta Garcia-Miralles,Arquimedes Cheffer,Patrick Bovio,Stefanie Heidrich,Tanja VogelPubMed 37759327
- Cell death discovery 9:3242023N-acetyltransferase 10 promotes cutaneous wound repair via the NF-κB-IL-6 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBen Wang,Jin Zhang,Guo Li,Chenzhong Xu,Langmei Yang,Jie Zhang,Yalan Wu,Ye Liu,Zuojun Liu,Ming Wang,Ji Li,Xiaolong Tang,Baohua LiuPubMed 37644005
- MedComm 4:e3032023M1 macrophage-derived exosomes promote autoimmune liver injury by transferring long noncoding RNA H19 to hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongting Zhang,Liang Hong,Xuehui Li,Yuyu Li,Xujun Zhang,Jingjing Jiang,Fan Shi,Hongyan DiaoPubMed 37398637
- Human cell 36:1755-17722023PSMD4 drives progression of hepatocellular carcinoma via Akt/COX2 pathway and p53 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiamin Zhang,Shu Fang,Fanghao Rong,Miaomiao Jia,Yunpeng Wang,Huixian Cui,Peipei HaoPubMed 37336868
- iScience 26:1071262023Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLalit R Patel,Sabrina A Stratton,Megan McLaughlin,Patrick Krause,Kendra Allton,Andrés López Rivas,Daniela Barbosa,Traver Hart,Michelle C BartonPubMed 37426340
- Molecular nutrition & food research 67:e22005332023Oleanolic Acid Inhibits SCD1 Gene Expression to Ameliorate Fructose-Induced Hepatosteatosis through SREBP1c-Dependent and -Independent Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Yao,Meng Wang,Jun Zhang,Xianqin Luo,Chunlin Yuan,Ruojun Bai,Tongzhuang Wang,Yumeng Xi,Chunli Li,Dazhi Ke,Johji Yamahara,Yuhao Li,Yongfen Yi,Shang Wang,Jianwei WangPubMed 36972071
- Drug design, development and therapy 17:1357-13692023UBE2T Promotes Temozolomide Resistance of Glioblastoma Through Regulating the Wnt/β-Catenin Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Wang,Ge Gao,Xiangpin Wei,Yang Zhang,Jian YuPubMed 37181827
- Heliyon 9:e156132023Influence of dose and exposition time in the effectiveness of -Acetyl-l-cysteine treatment in A549 human epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaula Montero,Inés Roger,Cristina Estornut,Javier Milara,Julio CortijoPubMed 37144195
- Medical oncology (Northwood, London, England) 40:1512023HIF1α-dependent and independent pathways regulate the expression of PD-L1 in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErasmia T Xanthopoulou,Christos Kakouratos,Christos Nanos,Anastasia G Gkegka,Christos Kalaitzis,Alexandra Giatromanolaki,Michael I KoukourakisPubMed 37067635
- Theranostics 13:2263-22802023OTUD1 promotes pathological cardiac remodeling and heart failure by targeting STAT3 in cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengyang Wang,Xue Han,Tianxiang Yu,Minxiu Wang,Wu Luo,Chunpeng Zou,Xiuyun Li,Gao Li,Gaojun Wu,Yi Wang,Guang LiangPubMed 37153745
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/300 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/300 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:28716252, PubMed:32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:28716252, PubMed:32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:28716252, PubMed:32910914).
Alternative names
LMN2, LMNB, LMNB1, Lamin-B1
Recommended products
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker is a rabbit recombinant monoclonal antibody that is used to detect Lamin B1 in ICC/IF, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with LMNB1 knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR8985(B) is cited in over 250 publications
- Sample sizes available
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8985(B)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.95 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has been cited over 189 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality manufacturing and validation processes ensure Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has been confirmed by testing in knockout samples.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has 14 independent reviews from customers.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) specifically detects Lamin B1 (UniProt ID: P20700; Molecular weight: 66kDa) and is sold in a convenient trial size to enable initial testing (20 uL) and larger sizes for subsequent scaling up experiments (100 uL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR8985(B) - ab22797.
Antibody clone EPR8985(B) is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, HRP, Alexa Fluor® 594 (ab19416, ab19418, ab19419, Alexa Fluor® 594 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker ab216723).
Lamin B1/LMNB1/LMNB is a critical component of the nuclear lamina, playing a vital role in chromatin organization and nuclear stability. Utilizing our lmnb1 antibody can significantly enhance the detection and study of Lamin B1. Research indicates that mutations in Lamin B1 are linked to demyelinating diseases, such as adult-onset autosomal dominant leukodystrophy (ADLD), highlighting its importance in maintaining neural health
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lamin B1 also known as LMNB1 is a protein that plays an important role in the structure of the nuclear lamina a dense fibrillar network inside the nucleus of cells. This protein belongs to the lamin family and has an approximate molecular weight of 66 kDa. Lamin B1 is expressed in nearly all types of cells acting as a structural component by interacting with chromatin and anchoring the nuclear envelope. It contributes to regulating nuclear stability and mechanical support which is essential for proper cell function.
- Biological function summary
Lamin B1 serves many important functions by providing structural integrity and regulating gene expression through chromatin organization. Part of the nuclear lamina complex Lamin B1 interacts with other lamin proteins such as Lamin A and Lamin C forming a mesh-like structure that supports the nuclear envelope. This protein also affects cellular processes such as DNA replication RNA transcription and cell division indicating its broad impact on various cellular activities.
- Pathways
Lamin B1 plays an important role in gene expression regulation and cell cycle control. It interacts with pathways involved in chromatin remodeling influencing the access of transcription factors to DNA which affects gene expression profiles. Lamin B1 associates with related proteins such as Lamin A/C and other nuclear matrix proteins to maintain cellular architecture stability and normal cell function. This organization is vital to ensure the accuracy of cellular processes and prevent anomalies during cell division.
- Associated diseases and disorders
Mutations or altered expression of Lamin B1 have been associated with several pathological conditions. One noteworthy disorder is autosomal dominant adult-onset leukodystrophy where Lamin B1 expression levels are abnormal. This protein also connects to multiple sclerosis through its role in maintaining nuclear architecture. Additionally changes in Lamin B1 expression levels can influence pathways involving Lamin A/C and potentially lead to other laminopathies highlighting its influence in maintaining cellular health and contributing to disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
18 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunocytochemistry-immunofluorescence-img119807.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
ab133741 staining Lamin B1 in wild-type HAP1 cells (top panel) and Lamin B1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab133741 at 1μg/ml dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudo-color red) overnight at +4°C. The cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)) at 1/1000 dilution for 1 hour. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunocytochemistry-immunofluorescence-img15024.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (Human Burkitt's lymphoma cell line) cells labeling Lamin B1 with ab133741 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/200 dilution (green). Nuclear envelope staining on Ramos cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab133741 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/200 dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111560.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of the bladder tissue labeling Lamin B1 with purified ab133741 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img142367.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Lanes 1- 2: Merged signal (red and green). Green - ab133741 observed at 66-70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.ab133741 was shown to react with LMNB1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human LMNB1 (Lamin B1) knockout HeLa cell line ab255404 (knockout cell lysate Human LMNB1 (Lamin B1) knockout HeLa cell lysate ab263825) was used. Wild-type HeLa and LMNB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133741 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: LMNB1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human LMNB1 (Lamin B1) knockout HeLa cell line (Human LMNB1 (Lamin B1) knockout HeLa cell line ab255404)
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 66-70 kDa
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img139633.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Lanes 1 - 4: Merged signal (red and green). Green - ab133741 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.ab133741 was shown to specifically react with Lamin B1 in wild type HAP1 cells. No band was observed when knockout samples were used. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. ab133741 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Lanes 1 and 3: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/1000 dilution
Lanes 2 and 4: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lanes 2 and 4: Empty
Lane 3: LMNB1 knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 66 kDa
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img43581.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Lanes 1 - 3: Merged signal (red and green).Green - Target observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
This western blot image is a comparison between ab133741 and a competitor's discontinued goat polyclonal antibody.
All lanes: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: Empty
Lane 3: Lamin B1 knockout HAP1 cell lysate at 20 µg
Predicted band size: 66 kDa
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img142907.png "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Lamin B1 Western blot staining of GST-tagged Recombinant Human Lamin B1 protein (aa 1 to 586) using rabbit Anti-Lamin B1 antibody
Recombinant Human Lamin B1 protein (ab114163)
All lanes: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/1000 dilution
All lanes: GST-tagged Recombinant Human Lamin B1 protein (aa 1 to 586) at 0.015 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 100 kDa
Exposure time: 1s
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img105003.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/50000 dilution
Lane 1: Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 20 µg
Lane 2: Molt-4 (Human lymphoblastic leukemia cell line) cell lysate at 20 µg
Lane 3: Y79 (Human retinoblastoma cell line) cell lysate at 20 µg
Lane 4: Caco-2 (Human colorectal adenocarcinoma cells) cell lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 66 kDa
Observed band size: 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115789.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Immunohistochemical staining of paraffin embedded Mouse Cerebral cortex with purified ab133741 at a working dilution of 1/300. The secondary antibody used is a HRP polymer for rabbit IgG. Nuclear envelope staining on neuron cells of Cerebral cortex tissue is observed. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunoprecipitation-img96884.jpg "Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
ab133741 (purified) at 1/20 immunoprecipitating Lamin B1 in Jurkat cells (Lane 1). For western blotting, ab133741 was used at 1/1000 dilution and an HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Predicted band size: 66 kDa
Observed band size: 70 kDa
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img104266.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/50000 dilution
Lane 1: C6 (Rat glial tumor cells) cell lysate at 20 µg
Lane 2: PC12 (Rat adrenal gland pheochromocytoma) cell lysate at 20 µg
Lane 3: NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 20 µg
Lane 4: RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 66 kDa
Observed band size: 70 kDa
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img83480.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/10000 dilution
All lanes: Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg
SecondaryAll lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 66 kDa
Observed band size: 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70247.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Lamin B1 with unpurified ab133741 at 1/250.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--oi-rd-scanning-img98203.jpg "OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img27508.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Blocking and Diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/10000 dilution
Lane 1: Mouse brain lysates at 10 µg
Lane 2: Mouse heart lysates at 10 µg
Lane 3: Mouse kidney lysates at 10 µg
Lane 4: Mouse spleen lysates at 10 µg
Lane 5: Rat brain lysates at 10 µg
Lane 6: Rat heart lysates at 10 µg
Lane 7: Rat spleen lysates at 10 µg
Lane 8: C6 (Rat glial tumor cells) whole cell lysates at 10 µg
Lane 9: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg
Lane 10: NIH/3T3 (Mouse embyro fibroblast cells) at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 66 kDa
Observed band size: 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88855.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lamin B1 with unpurified ab133741 at 1/250.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444532.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Immunohistochemical analysis of formalin fixed paraffin embedded human colon labelling Lamin B1 with ab133741 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
ab133741 anti-Lamin B1 antibody [EPR8985(B)] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img425359.jpg "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
Negative control: WEHI-231 (PMID:2842066).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Alternatively spliced forms of the EVI-1 gene encode at least three distinct proteins: EVI-1 (145 kDa), MDS1/EVI-1 (200 kDa) and EVI-1Δ324 (88 kDa) PMID:(PMID: 24944602).
The identity of the higher MW band at approximately 300 kDa is unknown.
In Western blot, Anti-Lamin B1 antibody [EPR8985 (B)] (ab133741) staining at 1/1000 dilution.
All lanes: Western blot - Anti-EVI1 antibody [EPR26816-18] (Anti-EVI1 antibody [EPR26816-18] ab315973) at 1/1000 dilution
Lane 1: MEF (mouse embryo fibroblast) whole cell lysate at 60 µg
Lane 2: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 60 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Observed band size: 75 kDa, 200 kDa, 70 kDa
Exposure time: 59s
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img43581.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img142907.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img105003.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115789.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunoprecipitation-img96884.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img104266.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img83480.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70247.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--oi-rd-scanning-img98203.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img27508.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88855.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444532.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
![Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741), expandable thumbnail](https://content.abcam.com/products/images/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741--western-blot-img425359.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com