Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker - Mouse IgG1 (Chimeric)

• WB

Lab

Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker - Mouse IgG1 (Chimeric) (AB324446)

False colour image of Western blot : Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker - Mouse IgG1 (Chimeric) (ab324446) staining at 1/1000 dilution, shown in black; Rabbit anti-GAPDH loading control staining, shown in magenta. In Western blot, ab324446 was shown to bind specifically to LMNB1. A band was observed at 66 kDa in Wild-type HeLa cell lysates with no signal observed at this size in LMNB1 knockout cell line. A band of the same size was also observed in mouse heart lysate. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1 : 10000 and Goat anti-Rabbit IgG H&L 680RD at 1 : 20000.

All lanes:

Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker - Mouse IgG1 (Chimeric) (ab324446) at 1/1000 dilution

Lane 1:

HeLa WT

Lane 2:

HeLa KO

Lane 3:

Mouse Heart

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-fc-hrp-preadsorbed-ab98717'>ab98717</a>) at 1/10000 dilution

Lanes 1 - 3:

Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Observed band size: 66 kDa

false

• WB

Lab

Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker - Mouse IgG1 (Chimeric) (AB324446)

False colour image of Western blot : Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker - Mouse IgG1 (Chimeric) (ab324446) staining at 1/1000 dilution, shown in black; Rabbit anti-GAPDH loading control staining, shown in magenta.

In Western blot, ab324446 was shown to bind specifically to LMNB1. A band was observed at 66 kDa in Wild-type HAP1 cell lysates with no signal observed at this size in LMNB1 knockout cell line. A band of the same size was also observed in mouse brain lysate and mouse heart lysate.

Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1 : 10000 and Goat anti-Rabbit IgG H&L 680RD at 1 : 20000.

All lanes:

Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker - Mouse IgG1 (Chimeric) (ab324446) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysates

Lane 2:

LMNB1 knockout HAP1 cell line

Lane 3:

Mouse brain lysate

Lane 4:

Mouse heart lysate

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-fc-hrp-preadsorbed-ab98717'>ab98717</a>) at 1/10000 dilution

Observed band size: 66 kDa

false