Anti-Lamin B1 antibody - Nuclear Envelope Marker is a rabbit polyclonal antibody that is used to detect Lamin B1 in ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with LMNB1 knockout cell line validation
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- Acta neuropathologica communications 11:2002023Targeting RACK1 to alleviate TDP-43 and FUS proteinopathy-mediated suppression of protein translation and neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeibei Zhao,Catherine M Cowan,Juliane A Coutts,Darren D Christy,Ananya Saraph,Shawn C C Hsueh,Stephen S Plotkin,Ian R Mackenzie,Johanne M Kaplan,Neil R CashmanPubMed 38111057
- Stem cell research & therapy 14:3672023JAK2 as a surface marker for enrichment of human pluripotent stem cells-derived ventricular cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLee Chuen Liew,Boon Min Poh,Omer An,Beatrice Xuan Ho,Christina Ying Yan Lim,Jeremy Kah Sheng Pang,Leslie Y Beh,Henry He Yang,Boon-Seng SohPubMed 38093391
- Nature communications 14:81602023Loss of cohesin regulator PDS5A reveals repressive role of Polycomb loops.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Bsteh,Hagar F Moussa,Georg Michlits,Ramesh Yelagandula,Jingkui Wang,Ulrich Elling,Oliver BellPubMed 38071364
- PLoS biology 21:e30024172023FMRP deficiency leads to multifactorial dysregulation of splicing and mislocalization of MBNL1 to the cytoplasm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuna Jung,Sneha Shah,Geongoo Han,Joel D RichterPubMed 38048343
- Cells 12:2023Lysine Demethylase KDM2A Promotes Proteasomal Degradation of TCF/LEF Transcription Factors in a Neddylation-Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTijana Šopin,František Liška,Tomáš Kučera,Dušan Cmarko,Tomáš VacíkPubMed 37998355
- Nature aging 3:1561-15752023Senolytic therapy alleviates physiological human brain aging and COVID-19 neuropathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulio Aguado,Alberto A Amarilla,Atefeh Taherian Fard,Eduardo A Albornoz,Alexander Tyshkovskiy,Marius Schwabenland,Harman K Chaggar,Naphak Modhiran,Cecilia Gómez-Inclán,Ibrahim Javed,Alireza A Baradar,Benjamin Liang,Lianli Peng,Malindrie Dharmaratne,Giovanni Pietrogrande,Pranesh Padmanabhan,Morgan E Freney,Rhys Parry,Julian D J Sng,Ariel Isaacs,Alexander A Khromykh,Guillermo Valenzuela Nieto,Alejandro Rojas-Fernandez,Thomas P Davis,Marco Prinz,Bertram Bengsch,Vadim N Gladyshev,Trent M Woodruff,Jessica C Mar,Daniel Watterson,Ernst J WolvetangPubMed 37957361
- eLife 12:2023GLI1 facilitates collagen-induced arthritis in mice by collaborative regulation of DNA methyltransferases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaoran Ge,Qianping Guo,Ying Zhou,Wenming Li,Wei Zhang,Jiaxiang Bai,Qing Wang,Huaqiang Tao,Wei Wang,Zhen Wang,Minfeng Gan,Yaozeng Xu,Huilin Yang,Bin Li,Dechun GengPubMed 37929702
- International journal of molecular sciences 24:2023Polysaccharide Reduces Inflammation and Aging Phenotypes in the Dermal Fibroblasts through the Activation of the NRF2/HO-1 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKento Takaya,Toru Asou,Kazuo KishiPubMed 37958685
- Journal of translational medicine 21:6902023Systematic in vivo candidate evaluation uncovers therapeutic targets for LMNA dilated cardiomyopathy and risk of Lamin A toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia Yee Tan,Pui Shi Chan,Hansen Tan,Sung Wei Tan,Chang Jie Mick Lee,Jiong-Wei Wang,Shu Ye,Hendrikje Werner,Ying Jie Loh,Yin Loon Lee,Matthew Ackers-Johnson,Roger S Y Foo,Jianming JiangPubMed 37840136
- Nature 622:627-6362023Apoptotic stress causes mtDNA release during senescence and drives the SASP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStella Victorelli,Hanna Salmonowicz,James Chapman,Helene Martini,Maria Grazia Vizioli,Joel S Riley,Catherine Cloix,Ella Hall-Younger,Jair Machado Espindola-Netto,Diana Jurk,Anthony B Lagnado,Lilian Sales Gomez,Joshua N Farr,Dominik Saul,Rebecca Reed,George Kelly,Madeline Eppard,Laura C Greaves,Zhixun Dou,Nicholas Pirius,Karolina Szczepanowska,Rebecca A Porritt,Huijie Huang,Timothy Y Huang,Derek A Mann,Claudio Akio Masuda,Sundeep Khosla,Haiming Dai,Scott H Kaufmann,Emmanouil Zacharioudakis,Evripidis Gavathiotis,Nathan K LeBrasseur,Xue Lei,Alva G Sainz,Viktor I Korolchuk,Peter D Adams,Gerald S Shadel,Stephen W G Tait,João F PassosPubMed 37821702
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Indian muntjac | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Mouse | Dilution info 0.1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Pig, Xenopus laevis, Indian muntjac, Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1 µg/mL | Notes We recommend Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773). |
Species Mouse | Dilution info 0.1 µg/mL | Notes We recommend Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773). |
Species Rat | Dilution info 0.1 µg/mL | Notes We recommend Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Pig, Xenopus laevis, Indian muntjac, Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1-1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Pig, Xenopus laevis, Indian muntjac, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:28716252, PubMed:32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:28716252, PubMed:32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:28716252, PubMed:32910914).
Alternative names
LMN2, LMNB, LMNB1, Lamin-B1
Recommended products
Anti-Lamin B1 antibody - Nuclear Envelope Marker is a rabbit polyclonal antibody that is used to detect Lamin B1 in ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with LMNB1 knockout cell line validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P and WB.
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) was first used in a scientific publication in 1997 and has been cited over 1182 times in peer reviewed journals. It's performance in Western blot and immunofluorescence in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) has high sensitivity and specificity.
The specificity of Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) has been confirmed by ICC/IF testing in Lamin B1 knockout HAP1 cells.
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) has 108 independent reviews from customers.
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) specifically detects Lamin B1 (UniProt ID: P14733; Molecular weight: 67kDa) and is sold in 100 ug selling sizes.
Top cited with >1500 citations. Lamin B1 is essential for studying nuclear envelope structure and function, as well as investigating diseases like laminopathies and certain cancers
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lamin B1 also known as LMNB1 is a protein component of the nuclear lamina a dense fibrillar network inside the nuclear envelope. It plays a critical structural role in maintaining nuclear integrity. Lamin B1 typically has a molecular weight of approximately 67 kDa. This protein is expressed in the cells of almost all tissues contributing to the maintenance and organization of the nuclear envelope. Researchers often use Western blot techniques to detect and analyze Lamin B1 size and expression levels in various biological samples.
- Biological function summary
The Lamin B1 protein supports nuclear stability and regulates chromatin organization. It is a component of the nuclear lamina complex interacting with other lamin proteins such as lamin A and lamin C to form a structured network. This network is essential for chromatin tethering and gene expression regulation influencing cellular processes at the transcriptional level. Its interactions with chromatin and other nuclear components highlight its significance in the overall functional architecture of the cell nucleus.
- Pathways
Lamin B1 interacts with molecules involved in the mitotic spindle assembly and DNA replication pathways. It plays an essential role in mitosis where it helps in the disassembly and reassembly of the nuclear envelope. Lamin B1’s interactions with proteins like lamin A/C and emerin highlight its participation in nuclear membrane dynamics and chromatin organization during the cell cycle.
- Associated diseases and disorders
Abnormalities in Lamin B1 levels or function associate with conditions like autosomal dominant leukodystrophy and certain types of cancer. Misregulation or mutation of LMNB1 can alter nuclear stability and gene expression contributing to disease pathology. Lamin B1 also connects with proteins like emerin where the disrupted interaction may lead to nuclear envelope-related muscular dystrophies. Understanding these connections is vital for elucidating the molecular basis of these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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12 product images
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
All lanes: Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1/1000 dilution
All lanes: Human Pancreatic cell line - whole cell lysate at 20 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit antibody at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 68 kDa
Exposure time: 30s
Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Blocking buffer: 3% Milk
Gel type: MOPS
Exposure Time: 2 minutes
Observed band size: 73 kDa
Additional bands: 46 kDa
All lanes: Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/mL
Lane 1: HeLa whole cell lysate at 10 µg
Lane 2: PC12 whole cell lysate at 10 µg
Lane 3: NIH 3T3 whole cell lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 66 kDa
Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: empty lane
Lane 3: KO HAP1 LMNB1 whole cell lysate (20 μg)
Lane 4: empty lane
Lanes 1 - 4: Merged signal (red and green). Green - ab16048 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.ab16048 was shown to specifically react with LMNB1 (Lamin B1) in wild type HAP1 cells. No band was observed when LMNB1 (Lamin B1) knockout samples were used. ab16048 LMNB1 (Lamin B1) and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 0.1 μg per mL and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Predicted band size: 66 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Lamin B1 Immunocytochemistry/ Immunofluorescence staining of HepG2 cells using rabbit Anti-Lamin B1 antibody
ab16048 staining Lamin B1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16048 at 0.1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Lamin B1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human normal Liver tissue using rabbit Anti-Lamin B1 antibody
IHC image of Lamin B1 staining in Human normal Liver formalin fixed paraffin embedded tissue section* performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6 epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16048 1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Lane 1: Wild-type HAP1 nuclear lysate (10 μg)
Lane 2: Lamin B1 knockout HAP1 nuclear lysate (10 μg)Lanes 1 and 2: Green signal from target - ab16048 observed at 68 kDa. Red signal from loading control - Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade ab10799 observed at 18 kDa.
ab16048 was shown to specifically react with lamin B1 in wild-type HAP1 cells. No band was observed knockout samples were used. Wild-type and lamin B1 knockout samples were subjected to SDS-PAGE. ab16048 and Anti-Histone H3 antibody [mAbcam 10799] - Nuclear Marker and ChIP Grade ab10799 (loading control to histone H3 at 0.1μg/mL) were both incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/mL
Lane 1: Wild-type HAP1 nuclear lysate at 10 µg
Lane 2: Lamin B1 knockout HAP1 nuclear lysate at 10 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa, 66 kDa
Observed band size: 68 kDa
Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
All lanes: Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1/1000 dilution
All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Alexa fluor goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 68-70 kDa
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
ab16048 staining Lamin B1 in human infantile fibromatosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 3 hours at room temperature; antigen retrieval was by heat mediation in Tris pH9. Samples were incubated with primary antibody (1/100 in TBS + 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, ItalyImmunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Lamin B1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Lamin B1 antibody
Human and mouse cells stained with ab16048 (1/500 dilution). The cells were fixed and permeabilized in 4% formaldehyde 0.2% Tritonm X100 for 10 minutes at room temperature then washed 3x in PBS.
A: HeLa cells + ab16048 (green)
B: HeLa cells counterstained with DAPI (blue)
C: 3T3 cells + ab16048 (green)
D: 3T3 cells counterstained with DAPI (blue)
Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, ItalyImmunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Lamin B1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Lamin B1 antibody
Human and mouse cells stained with ab16048 (1/500 dilution). The cells were fixed in 100% methanol for 6 minutes at -20°C then washed once in PBS.
A: HeLa cells + ab16048 (green)
B: HeLa cells counterstained with DAPI (blue)
C: 3T3 cells + ab16048 (green)
D: 3T3 cells counterstained with DAPI (blue)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Lamin B1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human liver using rabbit Anti-Lamin B1 antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded human liver labelling lamin B1 with ab16048 at a concentration of 0.2µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab16048 anti lamin B1 antibody was incubated at 37°C for 16min. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Lamin B1 Immunocytochemistry/ Immunofluorescence staining of Mouse vascular smooth muscle cells using rabbit Anti-Lamin B1 antibody
Immunocytochemistry analysis of formaldehyde-fixed NP40-permeabilized Mouse vascular smooth muscle cells staining with ab16048 at 1/100 dilution. Secondary antibody was Alexa Fluor® 488 donkey Anti-rabbit at 1/500 dilution. Samples were incubated with the primary antibody with 3% BSA in PBS for 12 hours at 4°C. Blocking was done using 3% BSA for 1 hour at 21°C.
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