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Rabbit Polyclonal Lamin B1 antibody. Suitable for IHC-P, ICC, WB, IHC-Fr and reacts with Human, Rat samples. Cited in 47 publications. Immunogen corresponding to Synthetic Peptide within Human LMNB1 aa 550 to C-terminus.


Images

Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986), expandable thumbnail
  • Immunocytochemistry - Anti-Lamin B1 antibody (AB65986), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody (AB65986), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody (AB65986), expandable thumbnail
  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB65986), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Human LMNB1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20700

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCWBIHC-Fr
Human
Tested
Tested
Tested
Expected
Rat
Expected
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
0.50000-1.00000 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
0.50000-1.00000 µg/mL
Notes

Acetone fixed.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
0.10000-0.50000 µg/mL
Notes

-

Species
Human
Dilution info
0.10000-0.50000 µg/mL
Notes

-

Tested
Tested

Species
Rat
Dilution info
0.50000-1.00000 µg/mL
Notes

Formalin or acetone fixed tissues.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:28716252, PubMed:32910914). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:28716252, PubMed:32910914). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:28716252, PubMed:32910914).

Alternative names

Recommended products

Rabbit Polyclonal Lamin B1 antibody. Suitable for IHC-P, ICC, WB, IHC-Fr and reacts with Human, Rat samples. Cited in 47 publications. Immunogen corresponding to Synthetic Peptide within Human LMNB1 aa 550 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Human LMNB1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20700
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Lamin B1 also known as LMNB1 is a protein that plays an important role in the structure of the nuclear lamina a dense fibrillar network inside the nucleus of cells. This protein belongs to the lamin family and has an approximate molecular weight of 66 kDa. Lamin B1 is expressed in nearly all types of cells acting as a structural component by interacting with chromatin and anchoring the nuclear envelope. It contributes to regulating nuclear stability and mechanical support which is essential for proper cell function.

Biological function summary

Lamin B1 serves many important functions by providing structural integrity and regulating gene expression through chromatin organization. Part of the nuclear lamina complex Lamin B1 interacts with other lamin proteins such as Lamin A and Lamin C forming a mesh-like structure that supports the nuclear envelope. This protein also affects cellular processes such as DNA replication RNA transcription and cell division indicating its broad impact on various cellular activities.

Pathways

Lamin B1 plays an important role in gene expression regulation and cell cycle control. It interacts with pathways involved in chromatin remodeling influencing the access of transcription factors to DNA which affects gene expression profiles. Lamin B1 associates with related proteins such as Lamin A/C and other nuclear matrix proteins to maintain cellular architecture stability and normal cell function. This organization is vital to ensure the accuracy of cellular processes and prevent anomalies during cell division.

Associated diseases and disorders

Mutations or altered expression of Lamin B1 have been associated with several pathological conditions. One noteworthy disorder is autosomal dominant adult-onset leukodystrophy where Lamin B1 expression levels are abnormal. This protein also connects to multiple sclerosis through its role in maintaining nuclear architecture. Additionally changes in Lamin B1 expression levels can influence pathways involving Lamin A/C and potentially lead to other laminopathies highlighting its influence in maintaining cellular health and contributing to disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab65986), expandable thumbnail

    Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab65986)

    Lamin B1 Western blot staining using rabbit Anti-Lamin B1 antibody

    All lanes: Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab65986)

    Lane 1: MCF-7 Cell lysate

    Lane 2: HeLa Cell lysate

    Predicted band size: 66 kDa

  • Immunocytochemistry - Anti-Lamin B1 antibody (ab65986), expandable thumbnail

    Immunocytochemistry - Anti-Lamin B1 antibody (ab65986)

    Immunocytochemistry of A549 Cells staining Lamin B1 with ab65986.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody (ab65986), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody (ab65986)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Mammary Cancer Tissue staining Lamin B1 with ab65986

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody (ab65986), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody (ab65986)

    Immunohistochemistry (Frozen sections) of Rat Intestine Tissue staining Lamin B1 with ab65986

  • Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab65986), expandable thumbnail

    Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab65986)

    Lamin B1 Western blot staining of rat brain tissue lysate using rabbit Anti-Lamin B1 antibody

    All lanes: Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab65986) at 1 µg/mL

    All lanes: rat brain tissue lysate

    Predicted band size: 66 kDa

    Observed band size: 70 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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