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AB155319

Anti-Lamin B2 antibody

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(5 Publications)

Rabbit Polyclonal Lamin B2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LMNB2 aa 250-600.

View Alternative Names

LMN2, LMNB2, Lamin-B2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody (AB155319)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody (AB155319)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Ovarian carcinoma tissue labeling Lamin B with ab155319 at 1/250 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody (AB155319)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody (AB155319)

Immunofluorescence of paraformaldehyde-fixed HeLa cells labeling Lamin B2 using ab155319 at 1/500 dilution (green). Bottom image shows costaining with DNA probe.

Western blot - Anti-Lamin B2 antibody (AB155319)
  • WB

Unknown

Western blot - Anti-Lamin B2 antibody (AB155319)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Lamin B2 antibody (ab155319) at 1/10000 dilution

All lanes:

U87-MG cell lysate at 30 µg

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LMNB2 aa 250-600. The exact immunogen used to generate this antibody is proprietary information.

Q03252

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamin B2 also referred to as 'ln43' or 'lmnB2' is a lamin molecule with a molecular weight of approximately 66 kDa. It is an important component of the nuclear lamina an essential structure that provides mechanical support to the nuclear envelope. Lamin B2 is expressed in most cell types and is particularly abundant in cells with high proliferative capacity such as those in the fetal brain and liver. The protein is encoded by the LMNB2 gene and functions as an integral part of the nuclear envelope's architecture by interacting with other nuclear proteins.
Biological function summary

Lamin B2 plays a critical role in maintaining nuclear stability and chromatin organization. It is a part of a larger complex that includes Lamin B and other nuclear matrix proteins. This structural matrix is important for chromosomal segregation during cell division ensuring that the genetic material is evenly distributed between daughter cells. Lamin B2 also facilitates processes such as DNA replication and repair by serving as a scaffold for anchoring various molecular components within the nucleus.

Pathways

Lamin B2 is implicated in the regulation of the mitotic spindle formation during cell division which is a part of the cell cycle pathway. It interacts with proteins such as Lamin A and other nucleoporins contributing to the proper formation of nuclear pore complexes. Furthermore it plays a role in the pathway of apoptosis by modulating the release of factors from the nucleus that trigger cell death mechanisms. These interactions emphasize its importance in maintaining cellular function and integrity.

Alterations in Lamin B2 expression or function have been linked to certain types of cancer such as glioblastoma as well as to laminopathies. Laminopathies are a group of disorders caused by mutations in lamin proteins leading to defects in nuclear structure and function. Besides aberrant Lamin B2 is associated with neurological disorders through its interactions with other nuclear envelope protein components like Lamin A/C. These connections highlight the importance of Lamin B2 in both physiological processes and pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed : 33033404). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed : 33033404). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed : 33033404).
See full target information LMNB2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Genome biology 25:77 PubMed38519987

2024

Depletion of lamins B1 and B2 promotes chromatin mobility and induces differential gene expression by a mesoscale-motion-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Emily M Pujadas Liwag,Xiaolong Wei,Nicolas Acosta,Lucas M Carter,Jiekun Yang,Luay M Almassalha,Surbhi Jain,Ali Daneshkhah,Suhas S P Rao,Fidan Seker-Polat,Kyle L MacQuarrie,Joe Ibarra,Vasundhara Agrawal,Erez Lieberman Aiden,Masato T Kanemaki,Vadim Backman,Mazhar Adli

iScience 26:106992 PubMed37378334

2023

AKT2-mediated nuclear deformation leads to genome instability during epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Rong Fan,Sung-Nian Chang,Ching-Tung Chu,Hong-Chen Chen

The Journal of cell biology 220: PubMed34550316

2021

Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Isabell Schneider,Marta de Ruijter-Villani,M Julius Hossain,Tom A E Stout,Jan Ellenberg

Life science alliance 4: PubMed34385357

2021

Tyrosine phosphorylation of lamin A by Src promotes disassembly of nuclear lamina in interphase.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Tung Chu,Yi-Hsuan Chen,Wen-Tai Chiu,Hong-Chen Chen

The Journal of biological chemistry : PubMed33187986

2020

A conserved acetylation switch enables pharmacological control of tubby-like protein stability.

Applications

Unspecified application

Species

Unspecified reactive species

Evan M Kerek,Kevin H Yoon,Shu Y Luo,Jerry Chen,Robert Valencia,Olivier Julien,Andrew J Waskiewicz,Basil P Hubbard
View all publications
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Product promise

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