AB97513

Anti-Lamin B2 antibody

Name: Anti-Lamin B2 antibody (ab97513) | Abcam
Brand: Abcam Limited
SKU: AB97513
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Lamin B2 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LMNB2 aa 200-500.

View Alternative Names

LMN2, LMNB2, Lamin-B2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody (AB97513)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody (AB97513)

Immunohistochemical analysis of paraffin-embedded cal27 xenograft tissue labelling LMNB2 protein with ab97513 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Supplier Data

Immunoprecipitation - Anti-Lamin B2 antibody (AB97513)

Lamin B2 antibody [N3C2], Internal immunoprecipitates Lamin B2 protein in IP experiments. Samples were separated by 7.5% SDS-PAGE
The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody diluted at 1 /1000 dilution.

All lanes:

Immunoprecipitation - Anti-Lamin B2 antibody (ab97513) at 3 µg

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

Control with 3 µg of preimmune Rabbit IgG

Lane 3:

Immunoprecipitated HepG2 whole cell lysate using ab97513

Secondary

All lanes:

Anti-Rabbit IgG (HRP)

Predicted band size: 70 kDa

false

Supplier Data

Western blot - Anti-Lamin B2 antibody (AB97513)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-Lamin B2 antibody (ab97513) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 70 kDa

false

Supplier Data

Western blot - Anti-Lamin B2 antibody (AB97513)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-Lamin B2 antibody (ab97513) at 1/500 dilution

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

GL261 whole cell lysates at 30 µg

Lane 3:

C8D30 whole cell lysates at 30 µg

Lane 4:

NIH-3T3 whole cell lysates at 30 µg

Lane 5:

BCL-1 whole cell lysates at 30 µg

Lane 6:

Raw264.7 whole cell lysates at 30 µg

Lane 7:

C2C12 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LMNB2 aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

Q03252

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/40000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/40000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamin B2 also referred to as 'ln43' or 'lmnB2' is a lamin molecule with a molecular weight of approximately 66 kDa. It is an important component of the nuclear lamina an essential structure that provides mechanical support to the nuclear envelope. Lamin B2 is expressed in most cell types and is particularly abundant in cells with high proliferative capacity such as those in the fetal brain and liver. The protein is encoded by the LMNB2 gene and functions as an integral part of the nuclear envelope's architecture by interacting with other nuclear proteins.

Biological function summary

Lamin B2 plays a critical role in maintaining nuclear stability and chromatin organization. It is a part of a larger complex that includes Lamin B and other nuclear matrix proteins. This structural matrix is important for chromosomal segregation during cell division ensuring that the genetic material is evenly distributed between daughter cells. Lamin B2 also facilitates processes such as DNA replication and repair by serving as a scaffold for anchoring various molecular components within the nucleus.

Pathways

Lamin B2 is implicated in the regulation of the mitotic spindle formation during cell division which is a part of the cell cycle pathway. It interacts with proteins such as Lamin A and other nucleoporins contributing to the proper formation of nuclear pore complexes. Furthermore it plays a role in the pathway of apoptosis by modulating the release of factors from the nucleus that trigger cell death mechanisms. These interactions emphasize its importance in maintaining cellular function and integrity.

Alterations in Lamin B2 expression or function have been linked to certain types of cancer such as glioblastoma as well as to laminopathies. Laminopathies are a group of disorders caused by mutations in lamin proteins leading to defects in nuclear structure and function. Besides aberrant Lamin B2 is associated with neurological disorders through its interactions with other nuclear envelope protein components like Lamin A/C. These connections highlight the importance of Lamin B2 in both physiological processes and pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed : 33033404). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed : 33033404). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed : 33033404).

See full target information LMNB2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal : PubMed33468570

2021

Disease modeling with human neurons reveals LMNB1 dysregulation underlying DYT1 dystonia.

Applications

Unspecified application

Species

Unspecified reactive species

Baojin Ding,Yu Tang,Shuaipeng Ma,Masuma Akter,Meng-Lu Liu,Tong Zang,Chun-Li Zhang

Cell 176:56-72.e15 PubMed30612743

2019

Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Michel Cioni,Julie Qiaojin Lin,Anne V Holtermann,Max Koppers,Maximilian A H Jakobs,Afnan Azizi,Benita Turner-Bridger,Toshiaki Shigeoka,Kristian Franze,William A Harris,Christine E Holt

Cell 148:752-64 PubMed22341447

2012

Local translation of extranuclear lamin B promotes axon maintenance.

Applications

ICC/IF

Species

Xenopus laevis

Byung C Yoon,Hosung Jung,Asha Dwivedy,Catherine M O'Hare,Krishna H Zivraj,Christine E Holt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lamin B2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody (AB97513)

Immunoprecipitation - Anti-Lamin B2 antibody (AB97513)

Western blot - Anti-Lamin B2 antibody (AB97513)

Western blot - Anti-Lamin B2 antibody (AB97513)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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