Name: Anti-Lamin B2 antibody [EPR9701(B)]
SKU: ab151735
Rating: 4 (2 reviews)

Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) is a rabbit monoclonal antibody that is used to detect Lamin B2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 30 publications

Related conjugates and formulations

ab200426
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Alexa Fluor® 488 Anti-Lamin B2 antibody [EPR9701(B)]
ab240124
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Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free
ab200427
Conjugated
Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)]
ab303113
Conjugated
PE Anti-Lamin B2 antibody [EPR9701(B)]
ab303114
Conjugated
APC Anti-Lamin B2 antibody [EPR9701(B)]
ab303115
Conjugated
HRP Anti-Lamin B2 antibody [EPR9701(B)]
ab308838
Conjugated
AP Anti-Lamin B2 antibody [EPR9701(B)]
ab312578
Conjugated
Alexa Fluor® 568 Anti-Lamin B2 antibody [EPR9701(B)]
ab312097
Conjugated
Alexa Fluor® 555 Anti-Lamin B2 antibody [EPR9701(B)]
ab310568
Conjugated
Alexa Fluor® 594 Anti-Lamin B2 antibody [EPR9701(B)]
ab321132
Conjugated
Alexa Fluor® 750 Anti-Lamin B2 antibody [EPR9701(B)]

Images

Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	IP	WB	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Expected	Tested	Expected
Rat	Tested	Expected	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes For unpurified use at 1/50 - 1/100. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes -
Species Rat	Dilution info 1/2000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes For unpurified use at 1/250 - 1/500.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes For unpurified use at 1/1000 - 1/10000.
Species Mouse	Dilution info 1/2000	Notes For unpurified use at 1/1000 - 1/10000.
Species Rat	Dilution info 1/2000	Notes For unpurified use at 1/1000 - 1/10000.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information LMNB2

Function

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:33033404). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:33033404). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:33033404).

Alternative names

LMN2, LMNB2, Lamin-B2

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR9701(B)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of Lamin B2?

Anti-Lamin B2 [EPR9701(B)] (ab151735) specifically detects a band for Lamin B2 (UniProt: Q03252) at a molecular weight of 68kDa.

Recommended positive controls

IP: HepG2 cell lysate;IHC: Human bladder cancer tissue; Mouse and rat testis; mouse kidney and cerebrum.ICC/IF: HepG2 cells;WB: HepG2 and HeLa cells, Mouse and rat testis and rat spleen lysates.Flow Cyt (intra): HepG2 cells.

Trusted by the scientific community

Anti-Lamin B2 [EPR9701(B)] (ab151735) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products

We have a range of other formats of antibody clone [EPR9701(B)] also available for your convenience:
ab151735, Alexa Fluor® 488 - ab200426, Alexa Fluor® 647 - ab200427, Carrier free - ab240124, PE - ab303113, APC - ab303114, HRP - ab303115, Alkaline Phosphatase - ab308838, Alexa Fluor® 594 - ab310568, Alexa Fluor® 555 - ab312097, Alexa Fluor® 568 - ab312578, Alexa Fluor® 750 - ab321132

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Lamin B2 also referred to as 'ln43' or 'lmnB2' is a lamin molecule with a molecular weight of approximately 66 kDa. It is an important component of the nuclear lamina an essential structure that provides mechanical support to the nuclear envelope. Lamin B2 is expressed in most cell types and is particularly abundant in cells with high proliferative capacity such as those in the fetal brain and liver. The protein is encoded by the LMNB2 gene and functions as an integral part of the nuclear envelope's architecture by interacting with other nuclear proteins.

Biological function summary

Lamin B2 plays a critical role in maintaining nuclear stability and chromatin organization. It is a part of a larger complex that includes Lamin B and other nuclear matrix proteins. This structural matrix is important for chromosomal segregation during cell division ensuring that the genetic material is evenly distributed between daughter cells. Lamin B2 also facilitates processes such as DNA replication and repair by serving as a scaffold for anchoring various molecular components within the nucleus.

Pathways

Lamin B2 is implicated in the regulation of the mitotic spindle formation during cell division which is a part of the cell cycle pathway. It interacts with proteins such as Lamin A and other nucleoporins contributing to the proper formation of nuclear pore complexes. Furthermore it plays a role in the pathway of apoptosis by modulating the release of factors from the nucleus that trigger cell death mechanisms. These interactions emphasize its importance in maintaining cellular function and integrity.

Associated diseases and disorders

Alterations in Lamin B2 expression or function have been linked to certain types of cancer such as glioblastoma as well as to laminopathies. Laminopathies are a group of disorders caused by mutations in lamin proteins leading to defects in nuclear structure and function. Besides aberrant Lamin B2 is associated with neurological disorders through its interactions with other nuclear envelope protein components like Lamin A/C. These connections highlight the importance of Lamin B2 in both physiological processes and pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Purified ab151735 at 1/20 dilution (0.5μg) immunoprecipitating Lamin B2 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab151735 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab151735 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 68 kDa
All lanes: Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Predicted band size: 33 kDa, 68 kDa
Observed band size: 47 kDa
Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Mouse testis lysate at 20 µg
Lane 3: Rat spleen lysate at 20 µg
Lane 4: Rat testis lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution
All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab151735 at 1:50 dilution (2.18 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^®594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab151735 at 1/20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder cancer tissue sections labeling Lamin B2 with Purified ab151735 at 1:100 dilution (1.09 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissues labelling Lamin B2 with ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse kidney. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissues labelling Lamin B2 with ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on rat testis. The section was incubated with ab151735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
"Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissues labelling Lamin B2 with ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse testis. The section was incubated with ab151735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissues labelling Lamin B2 with ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse cerebrum. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument