Anti-Lamin B2 antibody [EPR9701(B)]
- BOND RX™ Validated
- RabMAb
- Recombinant
- What is this?
4
(2 Reviews)
|
(48 Publications)
Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) is a rabbit monoclonal antibody detecting Lamin B2 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
View Alternative Names
LMN2, LMNB2, Lamin-B2
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab151735 at 1 : 50 dilution (2.18 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab151735 at 1/20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder cancer tissue sections labeling Lamin B2 with Purified ab151735 at 1 : 100 dilution (1.09 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- IP
Unknown
Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
Purified ab151735 at 1/20 dilution (0.5μg) immunoprecipitating Lamin B2 in HepG2 whole cell lysate.
Lane 1 (input) : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab151735 + HepG2 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab151735 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 68 kDa
All lanes:
Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)
Predicted band size: 33 kDa,68 kDa
Observed band size: 47 kDa
false
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
"Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissues labelling Lamin B2 with ab151735 at 1 : 2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse testis. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument"
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissues labelling Lamin B2 with ab151735 at 1 : 2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse cerebrum. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissues labelling Lamin B2 with ab151735 at 1 : 2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on rat testis. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument"
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissues labelling Lamin B2 with ab151735 at 1 : 2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse kidney. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
- WB
Unknown
Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
Blocking Buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
Mouse testis lysate at 20 µg
Lane 3:
Rat spleen lysate at 20 µg
Lane 4:
Rat testis lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
false
- WB
Unknown
Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (AB151735)
Blocking Buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution
All lanes:
HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
false
Related conjugates and formulations (10)
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Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Lamin B2 antibody [EPR9701(B)]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)]
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578 PE
PE Anti-Lamin B2 antibody [EPR9701(B)]
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660 APC
APC Anti-Lamin B2 antibody [EPR9701(B)]
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HRP Anti-Lamin B2 antibody [EPR9701(B)]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-Lamin B2 antibody [EPR9701(B)]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Lamin B2 antibody [EPR9701(B)]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Lamin B2 antibody [EPR9701(B)]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-Lamin B2 antibody [EPR9701(B)]
Reactivity data
Product details
What is this antibody validated in?
Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Lamin B2?
Anti-Lamin B2 [EPR9701(B)] (ab151735) specifically detects a band for Lamin B2 (UniProt: Q03252) at a molecular weight of 68kDa.
Trusted by the scientific community
Anti-Lamin B2 [EPR9701(B)] (ab151735) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR9701(B)] also available for your convenience: ab151735, Alexa Fluor® 488 - ab200426, Alexa Fluor® 647 - ab200427, Carrier free - ab240124, PE - ab303113, APC - ab303114, HRP - ab303115, Alkaline Phosphatase - ab308838, Alexa Fluor® 594 - ab310568, Alexa Fluor® 555 - ab312097, Alexa Fluor® 568 - ab312578, Alexa Fluor® 750 - ab321132
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Lamin B2 plays a critical role in maintaining nuclear stability and chromatin organization. It is a part of a larger complex that includes Lamin B and other nuclear matrix proteins. This structural matrix is important for chromosomal segregation during cell division ensuring that the genetic material is evenly distributed between daughter cells. Lamin B2 also facilitates processes such as DNA replication and repair by serving as a scaffold for anchoring various molecular components within the nucleus.
Pathways
Lamin B2 is implicated in the regulation of the mitotic spindle formation during cell division which is a part of the cell cycle pathway. It interacts with proteins such as Lamin A and other nucleoporins contributing to the proper formation of nuclear pore complexes. Furthermore it plays a role in the pathway of apoptosis by modulating the release of factors from the nucleus that trigger cell death mechanisms. These interactions emphasize its importance in maintaining cellular function and integrity.
Product protocols
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Target data
Publications (48)
Recent publications for all applications. Explore the full list and refine your search
The Journal of clinical investigation 135: PubMed40662365
2025
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Cancer medicine 14:e70970 PubMed40366008
2025
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STAR protocols 6:103633 PubMed39932849
2025
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Aging 17:203-216 PubMed39774004
2025
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Frontiers in cell and developmental biology 12:1447630 PubMed39524225
2024
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Journal of cell science 138: PubMed39501901
2024
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Aging cell 23:e14303 PubMed39113346
2024
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Cell reports 43:114493 PubMed39028622
2024
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Cellular and molecular life sciences : CMLS 81:141 PubMed38485766
2024
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Nature 619:176-183 PubMed37286593
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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