Rabbit Recombinant Monoclonal Lamin B2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunoprecipitation-img3782.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)")
![Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--western-blot-img16177.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)")
![Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--western-blot-img28067.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunocytochemistry-immunofluorescence-img72372.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)")
![Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--flow-cytometry-intracellular-img32998.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested | Expected | Expected |
| Rat | Tested | Expected | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
8 products for Alternative Version
Target data
Function
Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:33033404). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:33033404). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:33033404).
Alternative names
LMN2, LMNB2, Lamin-B2
Recommended products
Rabbit Recombinant Monoclonal Lamin B2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9701(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab240124 is the carrier-free version of Anti-Lamin B2 antibody [EPR9701(B)] ab151735.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lamin B2 also referred to as 'ln43' or 'lmnB2' is a lamin molecule with a molecular weight of approximately 66 kDa. It is an important component of the nuclear lamina an essential structure that provides mechanical support to the nuclear envelope. Lamin B2 is expressed in most cell types and is particularly abundant in cells with high proliferative capacity such as those in the fetal brain and liver. The protein is encoded by the LMNB2 gene and functions as an integral part of the nuclear envelope's architecture by interacting with other nuclear proteins.
- Biological function summary
Lamin B2 plays a critical role in maintaining nuclear stability and chromatin organization. It is a part of a larger complex that includes Lamin B and other nuclear matrix proteins. This structural matrix is important for chromosomal segregation during cell division ensuring that the genetic material is evenly distributed between daughter cells. Lamin B2 also facilitates processes such as DNA replication and repair by serving as a scaffold for anchoring various molecular components within the nucleus.
- Pathways
Lamin B2 is implicated in the regulation of the mitotic spindle formation during cell division which is a part of the cell cycle pathway. It interacts with proteins such as Lamin A and other nucleoporins contributing to the proper formation of nuclear pore complexes. Furthermore it plays a role in the pathway of apoptosis by modulating the release of factors from the nucleus that trigger cell death mechanisms. These interactions emphasize its importance in maintaining cellular function and integrity.
- Associated diseases and disorders
Alterations in Lamin B2 expression or function have been linked to certain types of cancer such as glioblastoma as well as to laminopathies. Laminopathies are a group of disorders caused by mutations in lamin proteins leading to defects in nuclear structure and function. Besides aberrant Lamin B2 is associated with neurological disorders through its interactions with other nuclear envelope protein components like Lamin A/C. These connections highlight the importance of Lamin B2 in both physiological processes and pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunoprecipitation-img3782.jpg "Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation.
Purified Anti-Lamin B2 antibody [EPR9701(B)] ab151735 at 1/20 dilution (0.5μg) immunoprecipitating Lamin B2 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): Anti-Lamin B2 antibody [EPR9701(B)] ab151735 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Lamin B2 antibody [EPR9701(B)] ab151735 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 68 kDaAll lanes: Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] (Anti-Lamin B2 antibody [EPR9701(B)] ab151735)
Predicted band size: 33 kDa, 68 kDa
Observed band size: 47 kDa
![Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--western-blot-img16177.jpg "Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation.
Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (Anti-Lamin B2 antibody [EPR9701(B)] ab151735) at 1/2000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Mouse testis lysate at 20 µg
Lane 3: Rat spleen lysate at 20 µg
Lane 4: Rat testis lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
![Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--western-blot-img28067.jpg "Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation.
Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (Anti-Lamin B2 antibody [EPR9701(B)] ab151735) at 1/2000 dilution
All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunocytochemistry-immunofluorescence-img72372.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab240124 at 1:50 dilution (2.18 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.![Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--flow-cytometry-intracellular-img32998.jpg "Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation.Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab240124 at 1/20 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112870.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder cancer tissue sections labeling Lamin B2 with Purified ab240124 at 1:100 dilution (1.09 μg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426033.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissues labelling Lamin B2 with Anti-Lamin B2 antibody [EPR9701(B)] ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse kidney. The section was incubated with Anti-Lamin B2 antibody [EPR9701(B)] ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426036.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
"This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissues labelling Lamin B2 with Anti-Lamin B2 antibody [EPR9701(B)] ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on rat testis. The section was incubated with Anti-Lamin B2 antibody [EPR9701(B)] ab151735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument"![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426035.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
"This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissues labelling Lamin B2 with Anti-Lamin B2 antibody [EPR9701(B)] ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse testis. The section was incubated with Anti-Lamin B2 antibody [EPR9701(B)] ab151735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument"![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426029.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
This data was developed using Anti-Lamin B2 antibody [EPR9701(B)] ab151735, the same antibody clone in a different buffer formulation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissues labelling Lamin B2 with Anti-Lamin B2 antibody [EPR9701(B)] ab151735 at 1:2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse cerebrum. The section was incubated with Anti-Lamin B2 antibody [EPR9701(B)] ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112870.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426033.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426036.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426035.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124), expandable thumbnail](https://content.abcam.com/products/images/lamin-b2-antibody-epr9701b-bsa-and-azide-free-ab240124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426029.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)")
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