Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

This data was developed using ab151735, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab240124 at 1 : 50 dilution (2.18 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

This data was developed using ab151735, the same antibody clone in a different buffer formulation.Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab240124 at 1/20 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

This data was developed using ab151735, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder cancer tissue sections labeling Lamin B2 with Purified ab240124 at 1 : 100 dilution (1.09 μg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IP

Unknown

Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

This data was developed using ab151735, the same antibody clone in a different buffer formulation.

Purified ab151735 at 1/20 dilution (0.5μg) immunoprecipitating Lamin B2 in HepG2 whole cell lysate.
Lane 1 (input) : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab151735 + HepG2 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab151735 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 68 kDa

All lanes:

Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] (<a href='/en-us/products/primary-antibodies/lamin-b2-antibody-epr9701b-ab151735'>ab151735</a>)

Predicted band size: 33 kDa,68 kDa

Observed band size: 47 kDa

false

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

"This data was developed using ab151735, the same antibody clone in a different buffer formulation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissues labelling Lamin B2 with ab151735 at 1 : 2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse testis. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument"

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

This data was developed using ab151735, the same antibody clone in a different buffer formulation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissues labelling Lamin B2 with ab151735 at 1 : 2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse cerebrum. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

"This data was developed using ab151735, the same antibody clone in a different buffer formulation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissues labelling Lamin B2 with ab151735 at 1 : 2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on rat testis. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument"

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

This data was developed using ab151735, the same antibody clone in a different buffer formulation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissues labelling Lamin B2 with ab151735 at 1 : 2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection) counterstainined with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Nuclear envelope staining on mouse kidney. The section was incubated with ab151735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• WB

Unknown

Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

This data was developed using ab151735, the same antibody clone in a different buffer formulation.

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (<a href='/en-us/products/primary-antibodies/lamin-b2-antibody-epr9701b-ab151735'>ab151735</a>) at 1/2000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Mouse testis lysate at 20 µg

Lane 3:

Rat spleen lysate at 20 µg

Lane 4:

Rat testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

• WB

Unknown

Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (AB240124)

This data was developed using ab151735, the same antibody clone in a different buffer formulation.

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (<a href='/en-us/products/primary-antibodies/lamin-b2-antibody-epr9701b-ab151735'>ab151735</a>) at 1/2000 dilution

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false