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Mouse Monoclonal Lamin B2 antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr and reacts with Human, Zebrafish samples. Cited in 46 publications. Immunogen corresponding to Cell preparation containing LMNB2 protein.


Images

Western blot - Anti-Lamin B2 antibody [LN43] (AB8983), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (AB8983), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (AB8983), expandable thumbnail
  • Flow Cytometry - Anti-Lamin B2 antibody [LN43] (AB8983), expandable thumbnail
  • Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (AB8983), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing LMNB2 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q03252

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-Fr
Human
Tested
Tested
Tested
Tested
Zebrafish
Expected
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/1000
Notes

-

Expected
Expected

Species
Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Zebrafish
Dilution info
1/1
Notes

(when used with an avidin-biotinylated HRP complex as detection agent).

Species
Human
Dilution info
1/1
Notes

(when used with an avidin-biotinylated HRP complex as detection agent).

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:33033404). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:33033404). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:33033404).

Alternative names

LMN2, LMNB2, Lamin-B2

Recommended products

Mouse Monoclonal Lamin B2 antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr and reacts with Human, Zebrafish samples. Cited in 46 publications. Immunogen corresponding to Cell preparation containing LMNB2 protein.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing LMNB2 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q03252
Clone number
LN43
Purification technique
Affinity purification Protein G
Specificity

This antibody reacts with an epitope located in the C-terminal part of lamin B2.
Lamins do not appear to be universally distributed among different cell and tissue types. Please see the listed positive controls for this antibody. Other cell/tissue types have not been tested.

Epitope
Epitope located in the C-terminal part of lamin B2.
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Lamin B2 also referred to as 'ln43' or 'lmnB2' is a lamin molecule with a molecular weight of approximately 66 kDa. It is an important component of the nuclear lamina an essential structure that provides mechanical support to the nuclear envelope. Lamin B2 is expressed in most cell types and is particularly abundant in cells with high proliferative capacity such as those in the fetal brain and liver. The protein is encoded by the LMNB2 gene and functions as an integral part of the nuclear envelope's architecture by interacting with other nuclear proteins.

Biological function summary

Lamin B2 plays a critical role in maintaining nuclear stability and chromatin organization. It is a part of a larger complex that includes Lamin B and other nuclear matrix proteins. This structural matrix is important for chromosomal segregation during cell division ensuring that the genetic material is evenly distributed between daughter cells. Lamin B2 also facilitates processes such as DNA replication and repair by serving as a scaffold for anchoring various molecular components within the nucleus.

Pathways

Lamin B2 is implicated in the regulation of the mitotic spindle formation during cell division which is a part of the cell cycle pathway. It interacts with proteins such as Lamin A and other nucleoporins contributing to the proper formation of nuclear pore complexes. Furthermore it plays a role in the pathway of apoptosis by modulating the release of factors from the nucleus that trigger cell death mechanisms. These interactions emphasize its importance in maintaining cellular function and integrity.

Associated diseases and disorders

Alterations in Lamin B2 expression or function have been linked to certain types of cancer such as glioblastoma as well as to laminopathies. Laminopathies are a group of disorders caused by mutations in lamin proteins leading to defects in nuclear structure and function. Besides aberrant Lamin B2 is associated with neurological disorders through its interactions with other nuclear envelope protein components like Lamin A/C. These connections highlight the importance of Lamin B2 in both physiological processes and pathologies.

Product promise

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6 product images

  • Western blot - Anti-Lamin B2 antibody [LN43] (ab8983), expandable thumbnail

    Western blot - Anti-Lamin B2 antibody [LN43] (ab8983)

    All lanes: Western blot - Anti-Lamin B2 antibody [LN43] (ab8983) at 1 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Lane 3: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

    Lane 4: HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa

    Observed band size: 37 kDa, 75 kDa

    Exposure time: 20min

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)

    ab8983 immunohistochemistry on a frozen section of human kidney showing nuclear lamina staining in the ductal epithelium.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)

    IHC-Fr of human kidney showing nuclear lamina staining in the ductal epithelium.

  • Flow Cytometry - Anti-Lamin B2 antibody [LN43] (ab8983), expandable thumbnail

    Flow Cytometry - Anti-Lamin B2 antibody [LN43] (ab8983)

    Overlay histogram showing HeLA cells stained with ab8983 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (Normal Goat Serum ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8983, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (ab8983), expandable thumbnail

    Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (ab8983)

    Lamin B2 - Nuclear Envelope Marker was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Lamin B2 - Nuclear Envelope Marker (ab8983) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8983.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 73kDa: Lamin B2

    All lanes: Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (ab8983)

    Predicted band size: 68 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (ab8983)

    IF staining of a 9 days old zebrafish embryo.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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