Anti-Lamin B2 antibody [LN43]

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (AB8983)

IHC-Fr of human kidney showing nuclear lamina staining in the ductal epithelium.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (AB8983)

ab8983 immunohistochemistry on a frozen section of human kidney showing nuclear lamina staining in the ductal epithelium.

• Flow Cyt

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Flow Cytometry - Anti-Lamin B2 antibody [LN43] (AB8983)

Overlay histogram showing HeLa cells stained with ab8983 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8983, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• IP

Unknown

Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (AB8983)

Lamin B2 - Nuclear Envelope Marker was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Lamin B2 - Nuclear Envelope Marker (ab8983) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8983.
Secondary : Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band : 73kDa : Lamin B2

All lanes:

Immunoprecipitation - Anti-Lamin B2 antibody [LN43] (ab8983)

Predicted band size: 68 kDa

false

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (AB8983)

IF staining of a 9 days old zebrafish embryo.

• WB

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Western blot - Anti-Lamin B2 antibody [LN43] (AB8983)

All lanes:

Western blot - Anti-Lamin B2 antibody [LN43] (ab8983) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 68 kDa

Observed band size: 37 kDa,75 kDa

true

Exposure time: 20min