Rabbit Polyclonal Lamin-A/C antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human LMNA aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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- Oncology letters 26:5332023LAMC2 is a potential prognostic biomarker for cholangiocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhaa Hoo Ong,Yao-Yu Hsieh,Hong-Yue Lai,Ding-Ping Sun,Tzu-Ju Chen,Steven Kuan-Hua Huang,Yu-Feng Tian,Chia-Lin Chou,Yow-Ling Shiue,Hung-Chang Wu,Ti-Chun Chan,Hsin-Hwa Tsai,Chien-Feng Li,Yu-Hsuan KuoPubMed 38020294
- The Journal of cell biology 221:2022Nucleoplasmic lamin C rapidly accumulates at sites of nuclear envelope rupture with BAF and cGAS.Applications:IHC-PReactive species:HumanYohei Kono,Stephen A Adam,Yuko Sato,Karen L Reddy,Yixian Zheng,Ohad Medalia,Robert D Goldman,Hiroshi Kimura,Takeshi ShimiPubMed 36301259
- Genome biology 22:3052021Lamin C is required to establish genome organization after mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianrong Wong,Victoria E Hoskins,Ashley J Melendez-Perez,Jennifer C Harr,Molly Gordon,Karen L ReddyPubMed 34775987
- International journal of molecular sciences 21:2020Perinuclear Lamin A and Nucleoplasmic Lamin B2 Characterize Two Types of Hippocampal Neurons through Alzheimer's Disease Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Gil et. al.PubMed 32155994
- Oxidative medicine and cellular longevity 2019:82396422019FA-97, a New Synthetic Caffeic Acid Phenethyl Ester Derivative, Protects against Oxidative Stress-Mediated Neuronal Cell Apoptosis and Scopolamine-Induced Cognitive Impairment by Activating Nrf2/HO-1 Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Wan et. al.PubMed 31885818
- Cells 7:2018Autophagic Removal of Farnesylated Carboxy-Terminal Lamin Peptides.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Lu et. al.PubMed 29690642
- Oncotarget 7:24700-182016Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts.Applications:ICC/IFReactive species:HumanVeronika Eisch,Xiang Lu,Diana Gabriel,Karima DjabaliPubMed 27015553
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LMNA aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P02545
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Target data
Function
Lamin-A/C. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:2188730, PubMed:22431096, PubMed:2344612, PubMed:23666920, PubMed:24741066, PubMed:31434876, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:24741066, PubMed:31548606, PubMed:37788673, PubMed:37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed:23990565, PubMed:25127216). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:2188730, PubMed:2344612). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed:10080180, PubMed:10580070, PubMed:10587585, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:12927431, PubMed:15317753, PubMed:18551513, PubMed:18611980, PubMed:22431096, PubMed:23666920, PubMed:31548606). Also invoved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed:31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:18551513, PubMed:22431096). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920). Prelamin-A/C. Prelamin-A/C can accelerate smooth muscle cell senescence (PubMed:20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed:20458013).
Alternative names
LMN1, LMNA, Prelamin-A/C
Recommended products
Rabbit Polyclonal Lamin-A/C antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human LMNA aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LMNA aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P02545
- Purification technique
- Affinity purification Protein A
- Specificity
ab125679 reacts strongly in immunoblotting with fragments corresponding to peptide 319-572aa of Lamin C.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lamin C also known as LMNA forms part of the nuclear lamina which provides structural support to the cell nucleus. It has a molecular weight of approximately 65 kDa. Lamin C is expressed in various cell types especially in differentiated cells within tissues such as muscle cardiac and certain epithelial cells. It helps to maintain nuclear stability and influences gene expression by interacting with chromatin.
- Biological function summary
Lamin C contributes to the architecture of the nuclear envelope as part of the lamina complex. This structural role influences the mechanical properties of cells which affects how cells respond to mechanical stress. Lamin C associates with proteins from the inner nuclear membrane and chromatin playing an essential role in nuclear organization. Disruptions in Lamin C function can affect the physical properties of nuclei impacting gene regulation and cell function.
- Pathways
Multiple processes integrate Lamin C including the mitogen-activated protein kinase (MAPK) signaling pathway and the PI3K/AKT pathway. In these pathways Lamin C can interact with proteins like MAPK and Akt which are important for transmitting signals that regulate cell growth survival and differentiation. In these processes Lamin C ensures proper cell cycle progression and nuclear structural integrity highlighting its significant involvement in cellular dynamics.
- Associated diseases and disorders
Mutations and malfunctions in Lamin C are connected to muscular dystrophies and cardiomyopathies. These diseases highlight the importance of Lamin C in maintaining muscle tissue integrity and proper cardiac function. Lamin C mutations often co-occur with dysfunctions in Lamin A as both arise from the same precursor protein leading to overlapping symptoms in conditions like Emery-Dreifuss muscular dystrophy and dilated cardiomyopathy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin C antibody - Nuclear Envelope Marker (ab125679)
Lamin C Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Lamin C antibody
ab125679, staining Lamin C in paraffin embedded Human skin tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin C antibody (ab125679)
Immunohistochemistry analysis of human skin and epithelium, staining Lamin C in the sweat glands with ab125679. Negative control with no primary antibody.
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