Anti-Laminin 2 alpha antibody 4H8-2 is a rat monoclonal antibody that is used to detect Laminin 2 alpha in IHC. Suitable for Mouse samples.
- Cited in over 70 publications
- Antibody clone 4H8-2 is the most widely used clone for Laminin 2 alpha on the market
Images
![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576), expandable thumbnail](https://content.abcam.com/products/images/laminin-2-alpha-antibody-4h8-2-ab11576--immunohistochemistry-frozen-sections-img119745.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576)")
![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576), expandable thumbnail](https://content.abcam.com/products/images/laminin-2-alpha-antibody-4h8-2-ab11576--immunohistochemistry-frozen-sections-img135521.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576)")
![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576), expandable thumbnail](https://content.abcam.com/products/images/laminin-2-alpha-antibody-4h8-2-ab11576--immunohistochemistry-frozen-sections-img116875.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576)")
Publications
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- Skeletal muscle 13:192023Electrical impedance myography detects dystrophin-related muscle changes in mdx mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTetsuaki Hiyoshi,Fuqiang Zhao,Rina Baba,Takeshi Hirakawa,Ryosuke Kuboki,Kazunori Suzuki,Yoshiro Tomimatsu,Patricio O'Donnell,Steve Han,Neta Zach,Masato NakashimaPubMed 37980539
- Cancer research 83:1299-13142023Active Remodeling of Capillary Endothelium via Cancer Cell-Derived MMP9 Promotes Metastatic Brain Colonization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthia A Karreman,Alexander T Bauer,Gergely Solecki,Anna S Berghoff,Chanté D Mayer,Katharina Frey,Nils Hebach,Manuel J Feinauer,Nicole L Schieber,Cedric Tehranian,Luc Mercier,Mahak Singhal,Varun Venkataramani,Marc C Schubert,Daniel Hinze,Michael Hölzel,Iris Helfrich,Dirk Schadendorf,Stefan W Schneider,Dana Westphal,Hellmut G Augustin,Jacky G Goetz,Yannick Schwab,Wolfgang Wick,Frank WinklerPubMed 36652557
- eLife 11:2022The mini-IDLE 3D biomimetic culture assay enables interrogation of mechanisms governing muscle stem cell quiescence and niche repopulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErik Jacques,Yinni Kuang,Allison P Kann,Fabien Le Grand,Robert S Krauss,Penney M GilbertPubMed 36537758
- Nature communications 13:77922022Muscle 4EBP1 activation modifies the structure and function of the neuromuscular junction in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeok-Ting J Ang,Elisa M Crombie,Han Dong,Kuan-Ting Tan,Adriel Hernando,Dejie Yu,Stuart Adamson,Seonyoung Kim,Dominic J Withers,Hua Huang,Shih-Yin TsaiPubMed 36526657
- Frontiers in physiology 13:9939952022p62/Sqstm1 rescue in muscle retards the progression of steatohepatitis in -null mice fed a high-fat diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIkuru Miura,Kosuke Okada,Akiko Ishii,Eiji Warabi,Takahisa Watahiki,Keii To,Hitoshi Shimano,Shunichi Ariizumi,Junichi ShodaPubMed 36439272
- Biomaterials 290:1218182022Scalable macroporous hydrogels enhance stem cell treatment of volumetric muscle loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIoannis Eugenis,Di Wu,Caroline Hu,Gladys Chiang,Ngan F Huang,Thomas A RandoPubMed 36209578
- Development (Cambridge, England) 149:2022Mural Wnt/β-catenin signaling regulates Lama2 expression to promote neurovascular unit maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaptarshi Biswas,Sanjid Shahriar,Nicholas P Giangreco,Panos Arvanitis,Markus Winkler,Nicholas P Tatonetti,William J Brunken,Tyler Cutforth,Dritan AgalliuPubMed 36098369
- Nucleic acids research 50:11401-114142022Enhanced exon skipping and prolonged dystrophin restoration achieved by TfR1-targeted delivery of antisense oligonucleotide using FORCE conjugation in mdx mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCody A Desjardins,Monica Yao,John Hall,Emma O'Donnell,Reshmii Venkatesan,Sean Spring,Aiyun Wen,Nelson Hsia,Peiyi Shen,Ryan Russo,Bo Lan,Tyler Picariello,Kim Tang,Timothy Weeden,Stefano Zanotti,Romesh Subramanian,Oxana Ibraghimov-BeskrovnayaPubMed 35944903
- Nature metabolism 4:995-10062022Systemic induction of senescence in young mice after single heterochronic blood exchange.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOk Hee Jeon,Melod Mehdipour,Tae-Hwan Gil,Minha Kang,Nicholas W Aguirre,Zachery R Robinson,Cameron Kato,Jessy Etienne,Hyo Gyeong Lee,Fatouma Alimirah,Vighnesh Walavalkar,Pierre-Yves Desprez,Michael J Conboy,Judith Campisi,Irina M ConboyPubMed 35902645
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e224582022Skeletal muscle fiber type-specific expressions of mechanosensors integrin-linked kinase, talin, and vinculin and their modulation by loading and environmental conditions in humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBernhard Andresen,Markus de Marees,Thorsten Schiffer,Wilhelm Bloch,Frank SuhrPubMed 35867073
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Full Length Protein corresponding to Mouse Lama2. The exact immunogen used to generate this antibody is proprietary information. Database link Q60675
Reactivity data
Select an application| IHC-Fr | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 4.00000-8.00000 µg/mL | Notes This concentration was determined for acetone-fixed frozen sections of human tongue. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Alternative names
Laminin subunit alpha-2, Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain, Lama2
Recommended products
Anti-Laminin 2 alpha antibody 4H8-2 is a rat monoclonal antibody that is used to detect Laminin 2 alpha in IHC. Suitable for Mouse samples.
- Cited in over 70 publications
- Antibody clone 4H8-2 is the most widely used clone for Laminin 2 alpha on the market
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Full Length Protein corresponding to Mouse Lama2. The exact immunogen used to generate this antibody is proprietary information. Database link Q60675
- Clone number
- 4H8-2
- Purification technique
- Affinity purification Protein A
- Epitope
- The epitope recognized by the antibody resides in the N-terminal portion of the alpha 2 chain of laminin.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Product SpecificationsAnti-Laminin 2 alpha antibody [4H8-2] (ab11576) is a rat monoclonal antibody and is validated for use in IHC-Fr in mouse samples.
Anti-Laminin 2 alpha antibody [4H8-2] (ab11576) specifically detects Laminin 2 alpha (UniProt ID: P24043; Molecular weight: 342kDa) and is sold in 25 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Laminin 2 alpha antibody [4H8-2] (ab11576) has high sensitivity and specificity.
Anti-Laminin 2 alpha antibody [4H8-2] (ab11576) has been cited over 72 times in peer reviewed journals and is trusted by the scientific community.
Anti-Laminin 2 alpha antibody [4H8-2] (ab11576) has 7 independent reviews from customers.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576), expandable thumbnail](https://content.abcam.com/products/images/laminin-2-alpha-antibody-4h8-2-ab11576--immunohistochemistry-frozen-sections-img119745.jpg "Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)
Immunohistochemistry (Frozen sections) analysis of Mouse muscle tissue labeling Laminin 2 alpha with ab11576 at 1/200 dilution. Tissue fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100 in PBS for 30 minutes. Tissue blocked with 10% goat serum for 1 hour at room temperature followed by incubation with the primary antibody in 2% goat serum, 0.2% triton X-100 in PBS overnight at 4°C. Goat anti-rat IgG (Alexa Fluor® 568) was used as the secondary antibody at 1/500 dilution (red).
![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576), expandable thumbnail](https://content.abcam.com/products/images/laminin-2-alpha-antibody-4h8-2-ab11576--immunohistochemistry-frozen-sections-img135521.jpg "Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)")
Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)
Immunohistochemical analysis of acetone-fixed frozen section of mouse tongue tissue labeling Laminin 2 alpha using ab11576 at 8 μg/mL, followed by a monoclonal Anti-Rat Kappa & Lamda light chains-FITC congugate at 1/40 dilution (Green).
![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576), expandable thumbnail](https://content.abcam.com/products/images/laminin-2-alpha-antibody-4h8-2-ab11576--immunohistochemistry-frozen-sections-img116875.jpg "Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)")
This image is courtesy of a customer review submitted by Dr Ling GuoImmunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)
ab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr. The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours. A rhodamine-conjugated goat anti-rat antibody was used as the secondary.
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