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AB174717

Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal LAMA3 antibody. Carrier free. Suitable for IHC-Fr and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LAMNA, LAMA3, Laminin subunit alpha-3, Epiligrin 170 kDa subunit, Epiligrin subunit alpha, Kalinin subunit alpha, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, Nicein subunit alpha, E170, LAMB2T, LAMNB2, LAMC2, Laminin subunit gamma-2, Cell-scattering factor 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma, CSF 140 kDa subunit, LAMNB1, LAMB3, Laminin subunit beta-3, Epiligrin subunit beta, Kalinin B1 chain, Kalinin subunit beta, Laminin B1k chain, Laminin-5 subunit beta, Nicein subunit beta

2 Images
Immunohistochemistry (Frozen sections) - Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (AB174717)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (AB174717)

This IHC data was generated using the same antibody clone in a different buffer formulation (ab78286).

IHC image of Laminin 5 staining in a section of frozen normal human colon*, performed on a Leica Bond™ system using the standard protocol F. No antigen retrieval step was performed prior to staining. The section was incubated with ab78286, 1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemistry (Frozen sections) - Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (AB174717)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (AB174717)

This IHC data was generated using the same antibody clone in a different buffer formulation (ab78286).

IHC image of Laminin 5 staining in a section of frozen normal human skin*, performed on a Leica Bond™ system using the standard protocol F. No antigen retrieval step was performed prior to staining. The section was incubated with ab78286, 1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P3H9-2

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1-5 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
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Product details

ab174717 is the carrier-free version of ab78286.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin 5 also known as laminin-332 is a heterotrimeric glycoprotein composed of alpha-3 beta-3 and gamma-2 chains with a molecular mass of about 460 kDa. Laminin 5 is expressed in the basement membrane zone of epithelial tissues playing a significant role in tissue structure. This molecule is widely present in areas such as skin lungs and other epithelial cell surfaces facilitating cellular functions like adhesion migration and differentiation. Researchers often use laminin antibodies including those for laminin IHC to visualize this molecule in various tissue samples.
Biological function summary

Laminin 5 forms part of the integrin complexes that modulate interactions between cells and the extracellular matrix. It provides critical structural support and influences cell behavior within the tissue microenvironment. Laminin 5 participates in the anchoring of keratinocytes to the underlying basement membrane which is essential for skin integrity and maintenance. Its interaction with laminin receptors on the cell surface strengthens the cellular attachment impacting the overall integrity and mechanical resistance of tissues.

Pathways

Laminin 5 is integral to the hemidesmosome assembly pathway and the MAPK/ERK signaling pathway. These pathways involve its interactions with integrins such as alpha-6 beta-4 and other proteins like dystroglycan. Laminin molecules including laminin 5 influence the downstream signaling of MAPK/ERK which governs processes like cellular proliferation and differentiation. The linkage with integrin-mediated signaling pathways allows laminin 5 to participate actively in cell adhesion mechanisms and responses to extracellular matrix changes.

Laminin 5 associates with junctional epidermolysis bullosa and cancer progression. Mutations in the genes encoding laminin 5 subunits lead to junctional epidermolysis bullosa a disorder marked by fragile skin and blister formations due to defective basement membrane anchoring. In cancer altered expression of laminin 5 facilitates tumor invasion and metastasis often involving interactions with matrix metalloproteinases (MMPs) and other extracellular matrix components. These connections underline the role of laminin 5 in pathological processes highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.. Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
See full target information LAMA3

Additional targets

LAMC2,LAMB3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 8:66796-66814 PubMed28977997

2017

Expression of hypothalamic neurohormones and their receptors in the human eye.

Applications

Unspecified application

Species

Unspecified reactive species

Sander R Dubovy,Maria P Fernandez,Jose J Echegaray,Norman L Block,Noriyuki Unoki,Roberto Perez,Irving Vidaurre,Richard K Lee,Mehrdad Nadji,Andrew V Schally
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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