AB242198

Anti-Laminin alpha 4/LAMA4 antibody [CL3183]

Name: Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198) | Abcam
Brand: Abcam Limited
SKU: AB242198
Price: 630 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(6 Publications)

Mouse Monoclonal Laminin alpha 4/LAMA4 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Laminin subunit alpha-4 aa 650-850.

View Alternative Names

Laminin subunit alpha-4, Laminin-14 subunit alpha, Laminin-8 subunit alpha, Laminin-9 subunit alpha, LAMA4

14 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Negative control - Formalin-fixed, paraffin-embedded human lymph node tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded human colon tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded mouse stomach tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded rat stomach tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded rat kidney tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded rat rectum tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded rat heart muscle tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded rat epididymis tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Formalin-fixed, paraffin-embedded mouse epididymis tissue stained for Laminin alpha 4/LAMA4 with ab242198 at a 1 : 200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

All lanes:

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198) at 1 µg/mL

Lane 1:

Recombinant human Laminin-411 protein

Lane 2:

Recombinant human Laminin-421 protein

Lane 3:

Recombinant human Laminin-511 protein

Lane 4:

Recombinant human Laminin-121 protein

Lane 5:

Recombinant human Laminin-221 protein

Lane 6:

Recombinant human Laminin-332 protein

Predicted band size: 202 kDa

false

Supplier Data

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

All lanes:

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (ab242198) at 1 µg/mL

All lanes:

Human placenta tissue lysate

Predicted band size: 202 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3183

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Laminin subunit alpha-4 aa 650-850. The exact immunogen used to generate this antibody is proprietary information.

Q16363

Epitope

Binds to an epitope located within the peptide sequence MANNLTNWSQNLQHF as determined by overlapping synthetic peptides.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 4 (LAMA4) also known as laminin-8 is a subunit of the laminin protein family which are essential components of the extracellular matrix. This protein has a molecular weight of approximately 210 kDa. LAMA4 mainly expresses in the vasculature including blood vessels and organs with high metabolic rates like the heart and skeletal muscles. It plays a significant role in maintaining the structural integrity of tissues by forming a network that supports cell adhesion and migration.

Biological function summary

Laminin alpha 4 interacts with other laminin subunits such as beta and gamma chains to form heterotrimeric laminin complexes. These laminins are important for cell differentiation proliferation and tissue repair. LAMA4 influences the basement membrane dynamics contributing to the biomechanical properties of cells. Through its binding to integrins and other cell surface receptors LAMA4 activates intracellular signaling cascades necessary for organogenesis and tissue regeneration.

Pathways

Laminin alpha 4 participates actively in the PI3K/AKT pathway important for cell survival and growth. It also plays a role in the MAPK signaling pathway which is important for cell differentiation and response to stress signals. Through these pathways LAMA4 interacts with various proteins including integrins and dystroglycan modulating essential cellular processes and maintaining cellular homeostasis.

LAMA4's role becomes prominent in cancer progression where altered expression can influence tumor invasion and metastasis. Furthermore it connects to cardiovascular disorders especially in influencing angiogenesis and repair of ischemic tissues. In these contexts LAMA4 relates to proteins like integrin alpha6 and beta1 which facilitate aberrant cellular activities in disease processes. Understanding LAMA4's involvement offers potential pathways for therapeutic intervention in these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information Laminin subunit alpha-4

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:283 PubMed40462244

2025

ECM remodeling by PDGFRβ+ dental pulp stem cells drives angiogenesis and pulp regeneration via integrin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tiankai Di,Lulu Wang,Chao Feng,Jinlong Xu,Bo Shao,Baixiang Cheng,Liying Wang,Xiao Zhang,Ling Zhang,Yujiang Chen

Nature communications 16:749 PubMed39820504

2025

A CD26 tendon stem progenitor cell population contributes to tendon repair and heterotopic ossification.

Applications

Unspecified application

Species

Unspecified reactive species

Siwen Chen,Yingxin Lin,Hao Yang,Zihao Li,Sifang Li,Dongying Chen,Wenjun Hao,Shuai Zhang,Hua Chao,Jingyu Zhang,Jianru Wang,Zemin Li,Xiang Li,Zhongping Zhan,Hui Liu

Communications biology 7:1427 PubMed39487340

2024

FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Sishi Liu,Man Gao,Xue Zhang,Jun Wei,Hong Cui

Heliyon 10:e36234 PubMed39253230

2024

Comprehensive analysis and experimental verification of the mechanism of anoikis related genes in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Bao,Dongqian Li,Xinyu Yang,Shiqi Ren,Haoxiang Ding,Chengfeng Guo,Jian Wan,Yicheng Xiong,MingYan Zhu,Yao Wang

Theranostics 10:10274-10289 PubMed32929348

2020

LAMA4 upregulation is associated with high liver metastasis potential and poor survival outcome of Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Biao Zheng,Jianhua Qu,Kenoki Ohuchida,Haimin Feng,Stephen Jun Fei Chong,Zilong Yan,Yicui Piao,Peng Liu,Nan Sheng,Daiki Eguchi,Takao Ohtsuka,Kazuhiro Mizumoto,Zhong Liu,Shazib Pervaiz,Peng Gong,Masafumi Nakamura

iScience 21:273-287 PubMed31677479

2019

Systematic Identification of Cell-Cell Communication Networks in the Developing Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Bilal N Sheikh,Olga Bondareva,Sukanya Guhathakurta,Tsz Hong Tsang,Katarzyna Sikora,Nadim Aizarani, Sagar,Herbert Holz,Dominic Grün,Lutz Hein,Asifa Akhtar

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin alpha 4/LAMA4 antibody [CL3183]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [CL3183] (AB242198)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise