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AB321978

Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61]

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Rabbit Recombinant Monoclonal Laminin alpha 4/LAMA4 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

View Alternative Names

Laminin subunit alpha-4, Laminin-14 subunit alpha, Laminin-8 subunit alpha, Laminin-9 subunit alpha, LAMA4

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on human spleen. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells of human placenta. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Human pancreatic carcinoma tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells of human pancreatic carcinoma. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells and smooth muscle of human colon. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells of human gastric adenocarcinoma. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human lung. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells of mouse cardiac muscle. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : weak staining on endothelial cells of mouse spleen. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Mouse pancreatic tumor tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells of mouse pancreatic tumor. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Laminin alpha 4/LAMA4 with ab321978 at 1/100 (5.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelial cells of mouse lung. The section was incubated with ab321978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • WB

Supplier Data

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen(PMID : 9346933)

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (ab321978) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 202 kDa,124 kDa

false

Exposure time: 36s

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • WB

Supplier Data

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : PANC-1(PMID : 32929348)

The molecular weight observed is consistent with what has been described in the literature(PMID : 32332107, PMID : 32929348)

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (ab321978) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 202-250 kDa,124 kDa

false

Exposure time: 180s

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)
  • WB

Supplier Data

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (AB321978)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : brain, kidney, spleen(PMID : 9346933)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] (ab321978) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 250 kDa,36 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-Laminin alpha 4/LAMA4 antibody [EPR28287-61] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28287-61

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>Based on the sequence of the immunogen, the antibody is not expected to cross-react with human LAMA1, LAMA2, LAMA3 or LAMA5 .</p>

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Complementary Products

Assay Kits

AB277457

Human Laminin alpha 4/LAMA4 ELISA Kit

ELISA - Human Laminin alpha 4/LAMA4 ELISA Kit (AB277457)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 4 (LAMA4) also known as laminin-8 is a subunit of the laminin protein family which are essential components of the extracellular matrix. This protein has a molecular weight of approximately 210 kDa. LAMA4 mainly expresses in the vasculature including blood vessels and organs with high metabolic rates like the heart and skeletal muscles. It plays a significant role in maintaining the structural integrity of tissues by forming a network that supports cell adhesion and migration.
Biological function summary

Laminin alpha 4 interacts with other laminin subunits such as beta and gamma chains to form heterotrimeric laminin complexes. These laminins are important for cell differentiation proliferation and tissue repair. LAMA4 influences the basement membrane dynamics contributing to the biomechanical properties of cells. Through its binding to integrins and other cell surface receptors LAMA4 activates intracellular signaling cascades necessary for organogenesis and tissue regeneration.

Pathways

Laminin alpha 4 participates actively in the PI3K/AKT pathway important for cell survival and growth. It also plays a role in the MAPK signaling pathway which is important for cell differentiation and response to stress signals. Through these pathways LAMA4 interacts with various proteins including integrins and dystroglycan modulating essential cellular processes and maintaining cellular homeostasis.

LAMA4's role becomes prominent in cancer progression where altered expression can influence tumor invasion and metastasis. Furthermore it connects to cardiovascular disorders especially in influencing angiogenesis and repair of ischemic tissues. In these contexts LAMA4 relates to proteins like integrin alpha6 and beta1 which facilitate aberrant cellular activities in disease processes. Understanding LAMA4's involvement offers potential pathways for therapeutic intervention in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information Laminin subunit alpha-4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com