AB77175

Anti-Laminin alpha 5/LAMA5 antibody [4B12]

Name: Anti-Laminin alpha 5/LAMA5 antibody [4B12] (ab77175) | Abcam
Brand: Abcam Limited
SKU: AB77175
Rating: 3 (1 reviews)

(1 Review)

(15 Publications)

Mouse Monoclonal Laminin alpha 5/LAMA5 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human LAMA5.

View Alternative Names

KIAA0533, KIAA1907, LAMA5, Laminin subunit alpha-5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Laminin alpha 5/LAMA5 antibody [4B12] (AB77175)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Laminin alpha 5/LAMA5 antibody [4B12] (AB77175)

Immunofluorescence of (A) Human embryonic lung alveolae epithelium and (B) Human kidney labelling Lamin alpha 5 with ab77175.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4B12

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human LAMA5.

O15230

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 5 (LAMA5) also known as laminin alpha-5 chain is an important component of the basement membrane which provides structural support to tissues. LAMA5 is a subunit of the laminin protein a large heterotrimeric glycoprotein complex involved in numerous cellular processes. The molecular weight of laminin alpha 5 can reach approximately 400 kDa. This protein is expressed widely across various tissues including the kidney lung and skin where it plays a mechanical role by mediating cell adhesion differentiation and migration by forming a scaffold-like structure at the cellular level.

Biological function summary

Laminin alpha 5 participates in the assembly of the laminin-511 complex which is vital for tissue-specific functions and maintaining cellular structures. This complex is important for epithelial and endothelial cell stability ensuring cohesive interactions with integrins and other cellular receptors. Laminin alpha 5 as part of this complex promotes development wound healing and angiogenesis impacting cell survival and regeneration by providing essential biochemical signals through its binding sites.

Pathways

Laminin alpha 5 significantly influences the PI3K/AKT and MAPK pathways which are critical for cell proliferation and survival. Within these pathways LAMA5 interacts with integrin receptors facilitating signal transduction. These pathways further associate laminin alpha 5 with proteins like FAK and Src kinase which mediate downstream signaling events influencing cellular growth and adhesion. These pathways link laminin alpha 5 to diverse cellular functions and regulatory processes.

Laminin alpha 5 has implications in diseases such as renal fibrosis and epidermolysis bullosa. Aberrant expression or mutations of LAMA5 can lead to defective basement membrane integrity affecting tissue stability and function. In renal fibrosis alterations in LAMA5 associate with disrupted kidney architecture while in epidermolysis bullosa laminin alpha 5 mutations lead to skin fragility. These conditions can also involve interactions with proteins like collagen IV and integrins contributing to the disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Plays a role in the regulation of skeletogenesis, through a mechanism that involves integrin-mediated signaling and PTK2B/PYK2 (PubMed : 33242826).

See full target information LAMA5

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 51:858-875 PubMed40678259

2025

Lung cancer intravasation-on-a-chip: Visualization and machine learning-assisted automatic quantification.

Applications

Unspecified application

Species

Unspecified reactive species

Christy Wing Tung Wong,Joyce Zhi Xuen Lee,Anna Jaeschke,Sammi Sze Ying Ng,Kwok Keung Lit,Ho-Ying Wan,Caroline Kniebs,Dai Fei Elmer Ker,Rocky S Tuan,Anna Blocki

Biomaterials research 27:32 PubMed37076899

2023

Stabilization and improved functionality of three-dimensional perfusable microvascular networks in microfluidic devices under macromolecular crowding.

Applications

Unspecified application

Species

Unspecified reactive species

Ho-Ying Wan,Jack Chun Hin Chen,Qinru Xiao,Christy Wingtung Wong,Boguang Yang,Benjamin Cao,Rocky S Tuan,Susan K Nilsson,Yi-Ping Ho,Michael Raghunath,Roger D Kamm,Anna Blocki

Neuropathology and applied neurobiology 49:e12877 PubMed36633103

2023

Molecular mechanisms in chloroquine-exposed muscle cells elucidated by combined proteomic and microscopic studies.

Applications

Unspecified application

Species

Unspecified reactive species

Vietxuan Phan,Denisa Hathazi,Corinna Preuße,Artur Czech,Erik Freier,Gerta Shema,René P Zahedi,Andreas Roos

Journal of the American Society of Nephrology : JASN 34:88-109 PubMed36167728

2022

Kidney Organoids Generated Using an Allelic Series of NPHS2 Point Variants Reveal Distinct Intracellular Podocin Mistrafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Aude Dorison,Irene Ghobrial,Alison Graham,Thanushi Peiris,Thomas A Forbes,Michael See,Mithun Das,Moin A Saleem,Catherine Quinlan,Kynan T Lawlor,Mirana Ramialison,Sara E Howden,Melissa H Little

Cells 11: PubMed36078126

2022

Influence of the Postmortem/Storage Time of Human Corneas on the Properties of Cultured Limbal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gaëtan Le-Bel,Pascale Desjardins,Christelle Gross,Sergio Cortez Ghio,Camille Couture,Lucie Germain,Sylvain L Guérin

Communications biology 5:155 PubMed35197546

2022

Ibrutinib reverses IL-6-induced osimertinib resistance through inhibition of Laminin α5/FAK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Zhujun Li,Conghua Lu,Jianghua Li,Kejun Zhang,Caiyu Lin,Xiaolin Tang,Zhulin Liu,Yimin Zhang,Rui Han,Yubo Wang,Mingxia Feng,Yuan Zhuang,Chen Hu,Yong He

Communications biology 4:1351 PubMed34857869

2021

GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening.

Applications

Unspecified application

Species

Unspecified reactive species

Jack Tuffin,Musleeha Chesor,Valeryia Kuzmuk,Tim Johnson,Simon C Satchell,Gavin I Welsh,Moin A Saleem

Journal of the American Society of Nephrology : JASN 32:1697-1712 PubMed33911000

2021

Autonomous Calcium Signaling in Human and Zebrafish Podocytes Controls Kidney Filtration Barrier Morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia Djenoune,Ritu Tomar,Aude Dorison,Irene Ghobrial,Heiko Schenk,Jan Hegermann,Lynne Beverly-Staggs,Alejandro Hidalgo-Gonzalez,Melissa H Little,Iain A Drummond

Science advances 7: PubMed33523862

2021

Recessive variants impair actin remodeling and cause glomerulopathy in humans and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Amar J Majmundar,Florian Buerger,Thomas A Forbes,Verena Klämbt,Ronen Schneider,Konstantin Deutsch,Thomas M Kitzler,Sara E Howden,Michelle Scurr,Ker Sin Tan,Mickaël Krzeminski,Eugen Widmeier,Daniela A Braun,Ethan Lai,Ihsan Ullah,Ali Amar,Amy Kolb,Kaitlyn Eddy,Chin Heng Chen,Daanya Salmanullah,Rufeng Dai,Makiko Nakayama,Isabel Ottlewski,Caroline M Kolvenbach,Ana C Onuchic-Whitford,Youying Mao,Nina Mann,Marwa M Nabhan,Seymour Rosen,Julie D Forman-Kay,Neveen A Soliman,Andreas Heilos,Renate Kain,Christoph Aufricht,Shrikant Mane,Richard P Lifton,Shirlee Shril,Melissa H Little,Friedhelm Hildebrandt

International journal of molecular sciences 21: PubMed32825463

2020

Differential Labeling of Glycoproteins with Alkynyl Fucose Analogs.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyu Ma,Hideyuki Takeuchi,Huilin Hao,Chizuko Yonekawa,Kazuki Nakajima,Masamichi Nagae,Tetsuya Okajima,Robert S Haltiwanger,Yasuhiko Kizuka

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin alpha 5/LAMA5 antibody [4B12]

Immunocytochemistry/ Immunofluorescence - Anti-Laminin alpha 5/LAMA5 antibody [4B12] (AB77175)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise