AB210957

Anti-Laminin alpha 5/LAMA5 antibody [CL3118]

Name: Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (ab210957) | Abcam
Brand: Abcam Limited
SKU: AB210957

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(2 Publications)

Mouse Monoclonal Laminin alpha 5/LAMA5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LAMA5 aa 2550-2600.

View Alternative Names

KIAA0533, KIAA1907, LAMA5, Laminin subunit alpha-5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human lymph nodes tissue (negative control), labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human cervix tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human heart tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.

Supplier Data

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

All lanes:

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (ab210957) at 1/500 dilution

Lane 1:

Recombinant protein fragment of Human Laminin 511

Lane 2:

Recombinant protein fragment of Human Laminin 521

Lane 3:

Recombinant protein fragment of Human Laminin 121

Lane 4:

Recombinant protein fragment of Human Laminin 221

Lane 5:

Recombinant protein fragment of Human Laminin 332

Lane 6:

Recombinant protein fragment of Human Laminin 411

Predicted band size: 400 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3118

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LAMA5 aa 2550-2600. The exact immunogen used to generate this antibody is proprietary information.

O15230

Epitope

ab210957 binds to an epitope located within the peptide sequence RQGLVDRAQQ (aa 2555 - 2564).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 5 (LAMA5) also known as laminin alpha-5 chain is an important component of the basement membrane which provides structural support to tissues. LAMA5 is a subunit of the laminin protein a large heterotrimeric glycoprotein complex involved in numerous cellular processes. The molecular weight of laminin alpha 5 can reach approximately 400 kDa. This protein is expressed widely across various tissues including the kidney lung and skin where it plays a mechanical role by mediating cell adhesion differentiation and migration by forming a scaffold-like structure at the cellular level.

Biological function summary

Laminin alpha 5 participates in the assembly of the laminin-511 complex which is vital for tissue-specific functions and maintaining cellular structures. This complex is important for epithelial and endothelial cell stability ensuring cohesive interactions with integrins and other cellular receptors. Laminin alpha 5 as part of this complex promotes development wound healing and angiogenesis impacting cell survival and regeneration by providing essential biochemical signals through its binding sites.

Pathways

Laminin alpha 5 significantly influences the PI3K/AKT and MAPK pathways which are critical for cell proliferation and survival. Within these pathways LAMA5 interacts with integrin receptors facilitating signal transduction. These pathways further associate laminin alpha 5 with proteins like FAK and Src kinase which mediate downstream signaling events influencing cellular growth and adhesion. These pathways link laminin alpha 5 to diverse cellular functions and regulatory processes.

Laminin alpha 5 has implications in diseases such as renal fibrosis and epidermolysis bullosa. Aberrant expression or mutations of LAMA5 can lead to defective basement membrane integrity affecting tissue stability and function. In renal fibrosis alterations in LAMA5 associate with disrupted kidney architecture while in epidermolysis bullosa laminin alpha 5 mutations lead to skin fragility. These conditions can also involve interactions with proteins like collagen IV and integrins contributing to the disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Plays a role in the regulation of skeletogenesis, through a mechanism that involves integrin-mediated signaling and PTK2B/PYK2 (PubMed : 33242826).

See full target information LAMA5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:7902-7914 PubMed38709270

2024

Improvement of skin wound healing by giant salamander skin mucus gel wrapped with bone marrow mesenchymal stem cells via affecting integrin family molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Dayong Du,Yan Huang,Cui Xu,Yang Liu,Xiaohong Wu,Jie Yang,Zhipeng Liu,Jianxin Ma,Chaoji Huangfu

Clinical kidney journal 17:sfae037 PubMed38455522

2024

Characterization of glomerular basement membrane components within pediatric glomerular diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Chen,Xindi Zhou,Chun Gan,Qing Yang,Wanbing Chen,Xiaoqian Feng,Tao Zhang,Li Zhang,Lujun Dai,Yaxi Chen,Haiping Yang,Mo Wang,Wei Jiang,Qiu Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin alpha 5/LAMA5 antibody [CL3118]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (AB210957)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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