AB184330

Anti-Laminin alpha 5/LAMA5 antibody [EPR18919]

Name: Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) | Abcam
Brand: Abcam Limited
SKU: AB184330
Price: 330 USD
Availability: InStock
Rating: 4 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(8 Publications)

Rabbit Recombinant Monoclonal Laminin alpha 5/LAMA5 antibody. Suitable for WB and reacts with Recombinant fragment - Mouse, Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

KIAA0533, KIAA1907, LAMA5, Laminin subunit alpha-5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

3 Images

Unknown

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (AB184330)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 12559092; PMID : 9624186).

All lanes:

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 400 kDa

Observed band size: 404 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (AB184330)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 and 2 : 30 seconds; Lane 3 and 4 : 3 minutes.

The expression profile observed is consistent with what has been described in the literature (PMID : 12559092; PMID : 9624186).

Negative control : mouse liver (PMID : 7499364).

All lanes:

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

Lane 1:

Mouse P5 fetal kidney lysate at 20 µg

Lane 2:

Mouse P3 fetal lung lysate at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Lane 4:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 400 kDa

Observed band size: 404 kDa

false

Supplier Data

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (AB184330)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 15 seconds; Lane 2 : 3 minutes.

Mouse Laminin alpha 5/LAMA5 fragment recombinant protein contains aa46-304 with a His-Tag; Mouse Laminin alpha 3 fragment recombinant protein contains aa43-295 with a His-Tag.

All lanes:

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

Lane 1:

Mouse Laminin alpha 5/LAMA5 fragment recombinant protein at 0.01 µg

Lane 2:

Mouse Laminin alpha 3 fragment recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 400 kDa

false

Carrier free

Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18919

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts weakly with Laminin alpha 3

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant fragment - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin alpha 5 (LAMA5) also known as laminin alpha-5 chain is an important component of the basement membrane which provides structural support to tissues. LAMA5 is a subunit of the laminin protein a large heterotrimeric glycoprotein complex involved in numerous cellular processes. The molecular weight of laminin alpha 5 can reach approximately 400 kDa. This protein is expressed widely across various tissues including the kidney lung and skin where it plays a mechanical role by mediating cell adhesion differentiation and migration by forming a scaffold-like structure at the cellular level.

Biological function summary

Laminin alpha 5 participates in the assembly of the laminin-511 complex which is vital for tissue-specific functions and maintaining cellular structures. This complex is important for epithelial and endothelial cell stability ensuring cohesive interactions with integrins and other cellular receptors. Laminin alpha 5 as part of this complex promotes development wound healing and angiogenesis impacting cell survival and regeneration by providing essential biochemical signals through its binding sites.

Pathways

Laminin alpha 5 significantly influences the PI3K/AKT and MAPK pathways which are critical for cell proliferation and survival. Within these pathways LAMA5 interacts with integrin receptors facilitating signal transduction. These pathways further associate laminin alpha 5 with proteins like FAK and Src kinase which mediate downstream signaling events influencing cellular growth and adhesion. These pathways link laminin alpha 5 to diverse cellular functions and regulatory processes.

Laminin alpha 5 has implications in diseases such as renal fibrosis and epidermolysis bullosa. Aberrant expression or mutations of LAMA5 can lead to defective basement membrane integrity affecting tissue stability and function. In renal fibrosis alterations in LAMA5 associate with disrupted kidney architecture while in epidermolysis bullosa laminin alpha 5 mutations lead to skin fragility. These conditions can also involve interactions with proteins like collagen IV and integrins contributing to the disease pathology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Plays a role in the regulation of skeletogenesis, through a mechanism that involves integrin-mediated signaling and PTK2B/PYK2 (PubMed : 33242826).

See full target information LAMA5

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of biological methods 12:e99010066 PubMed40937375

2025

An experimental workflow for investigating anoikis resistance in cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Han,Yipan Zheng,Xiaohui Si,Zhe Liu

Cell reports 44:115381 PubMed40080499

2025

YAP1 is a key regulator of EWS::FLI1-dependent malignant transformation upon IGF-1-mediated reprogramming of bone mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rahil Noorizadeh,Barbara Sax,Tahereh Javaheri,Branka Radic-Sarikas,Valerie Fock,Veveeyan Suresh,Maximilian Kauer,Aleksandr Bykov,Danijela Kurija,Michaela Schlederer,Lukas Kenner,Gerhard Weber,Wolfgang Mikulits,Florian Halbritter,Richard Moriggl,Heinrich Kovar

Functional & integrative genomics 25:59 PubMed40072648

2025

MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Jiazhong Xu,Hongxia Zhang,Xiaobo Guo,Hongjun Liu,Qinhui Sun

Biomedical journal 47:100627 PubMed37390938

2023

LAMA5: A new pathogenic gene for non-syndromic cleft lip with or without cleft palate.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Fu,Yan Qi,Ling-Fa Xue,Yao-Xiang Xu,Jin Yue,Jin-Ze Zhao,Cong Li,Wenlin Xiao

Communications biology 5:155 PubMed35197546

2022

Ibrutinib reverses IL-6-induced osimertinib resistance through inhibition of Laminin α5/FAK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Zhujun Li,Conghua Lu,Jianghua Li,Kejun Zhang,Caiyu Lin,Xiaolin Tang,Zhulin Liu,Yimin Zhang,Rui Han,Yubo Wang,Mingxia Feng,Yuan Zhuang,Chen Hu,Yong He

International journal of molecular sciences 23: PubMed35008625

2021

Regulation and Function of Laminin A5 during Mouse and Human Decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Shan Yang,Hai-Yang Pan,Wen-Wen Shi,Si-Ting Chen,Ying Wang,Meng-Yuan Li,Hai-Yi Zhang,Chen Yang,Ai-Xia Liu,Zeng-Ming Yang

Journal of neuroinflammation 16:192 PubMed31660977

2019

NDP-MSH binding melanocortin-1 receptor ameliorates neuroinflammation and BBB disruption through CREB/Nr4a1/NF-κB pathway after intracerebral hemorrhage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Wu,Siming Fu,Yun Liu,Hansheng Luo,Feng Li,Yiying Wang,Meng Gao,Yuan Cheng,Zongyi Xie

Cellular physiology and biochemistry : internation 51:2030-2040 PubMed30522103

2018

Down-Regulation of Laminin (LN)- α5 is Associated with Preeclampsia and Impairs Trophoblast Cell Viability and Invasiveness Through PI3K Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Mei Zhang,Xi Xiong,Chao Tong,Qin Li,Shuai Huang,Qing Shu Li,Ya Ming Liu,Hai Ying Li,Phillip Baker,Nan Shan,Hong Bo Qi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin alpha 5/LAMA5 antibody [EPR18919]

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (AB184330)

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (AB184330)

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (AB184330)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise