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AB313469

Anti-Laminin antibody [RP23040175]

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Rabbit Recombinant Multiclonal Laminin gamma 3 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LAMC3.

View Alternative Names

Laminin subunit gamma-3, Laminin-12 subunit gamma, Laminin-14 subunit gamma, Laminin-15 subunit gamma, LAMC3

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Laminin Antibody [RP23040175] (AB313469)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Laminin Antibody [RP23040175] (AB313469)

Immunofluorescence analysis was performed on fixed and permeabilized C2C12 cells for detection of endogenous Laminin 213 using ab313469 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate ( 1 : 2000). Panel a) shows representative cells that were stained for detection and localization of Laminin 213 protein (green), Panel b) is stained for nuclei (blue) using with DAPI . Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin ( 1 : 300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of Laminin 213 in the extracellular matrix. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040175

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LAMC3.

Q9Y6N6

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Anti-alpha smooth muscle Actin antibody [1A4]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin is an important protein in the extracellular matrix serving as a structural scaffold in tissues. It has several alternate names including laminine UK and laminin-1. Composed of three polypeptide chains laminin has a large molecular mass of around 850 kDa. One prominently finds laminin in the basement membranes of various tissues where it plays a critical role in maintaining tissue architecture and providing a foundation for cell attachment and growth. It interacts with cell surface receptors including integrins which help mediate cellular responses to the surrounding environment.
Biological function summary

Laminin functions by influencing cellular behaviors such as adhesion migration differentiation and proliferation. It is a pivotal component of basement membrane complexes and interacts directly with other extracellular matrix proteins like fibronectin and collagen. This interaction helps to organize the matrix and supports the structural integrity and functionality of tissues. Laminin's biological activity profoundly impacts processes such as embryonic development and wound healing where it guides cells to necessary locations and cues them to assume specific roles.

Pathways

Laminin is integral to the regulation of cell signaling pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are critical for controlling cellular survival growth and differentiation. Laminin's interactions with integrin receptors facilitate signal transduction through these pathways promoting cellular outcomes appropriate for tissue repair and regeneration. Additionally laminin serves functions alongside other proteins like collagen in stabilizing the extracellular matrix contributing to the maintenance of tissue homeostasis and repair.

Laminin is linked to conditions like muscular dystrophy and certain types of cancer. Mutations or abnormalities in the laminin protein can disrupt muscle fiber integrity leading to degenerative disorders such as congenital muscular dystrophies. Similarly changes in laminin expression can facilitate cancer metastasis by altering cell adhesion and migration properties. In these contexts laminin's interactions with integrin and its influence on cell movement and survival become central to the disease's progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information LAMC3
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Product promise

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For full details, please see our Terms & Conditions

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