AB69633

Anti-Laminin beta 1 antibody

Name: Anti-Laminin beta 1 antibody (ab69633) | Abcam
Brand: Abcam Limited
SKU: AB69633
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal LAMB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LAMB1.

View Alternative Names

Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody (AB69633)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody (AB69633)

Immunofluorescence analysis of HeLa cells using ab69633 at a 1 : 500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 1 antibody (AB69633)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 1 antibody (AB69633)

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab69633 at 1 : 50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

Unknown

Western blot - Anti-Laminin beta 1 antibody (AB69633)

All lanes:

Western blot - Anti-Laminin beta 1 antibody (ab69633) at 1/500 dilution

Lane 1:

Extracts from HepG2 cells at 5 µg

Lane 2:

Extracts from HepG2 cells at 5 µg with immunising peptide

Predicted band size: 198 kDa

Observed band size: 110 kDa,130 kDa,60 kDa,67 kDa,>170 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human LAMB1. The exact immunogen used to generate this antibody is proprietary information.

P07942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 1 also known as LAMB1 is a subunit of the laminin molecule an essential component of the extracellular matrix. This protein has an approximate molecular weight of 200 kDa. Laminin beta 1 is one of the three chains that form the laminin heterotrimer. It is predominantly expressed in basement membranes which provide structural support and segregate tissues. The laminin 1 (laminin-111) complex including the LAMB1 chain contributes to cell adhesion differentiation migration and plays a role in tissue development and repair.

Biological function summary

This component of the laminin molecule plays a critical role in maintaining the integrity of tissues by organizing cell architecture and mediating cellular signals. Laminin beta 1 is part of a larger laminin protein complex that interacts with a variety of cell surface receptors including integrins and dystroglycan contributing to cell communication and signal transduction. These interactions guide cell orientation and regulate vital processes such as cell movement and growth.

Pathways

Laminin beta 1 participates in the integrin signaling pathway and the PI3K-AKT signaling pathway. It interacts closely with integrin proteins such as integrin alpha-6 and integrin beta-4 during these processes. In integrin signaling the laminin molecule facilitates the binding between cells and their surrounding extracellular matrix. This connection influences cellular responses including survival and motility which are necessary for normal development and wound healing processes.

Alterations in laminin beta 1 expression are linked to conditions like muscular dystrophy and Pierson syndrome. In muscular dystrophy the dysfunction of laminin beta 1 interacts with proteins such as dystrophin in muscle cells contributing to compromised structural integrity and muscle wasting. Similarly in Pierson syndrome abnormalities in laminin beta 1 are associated with a rare genetic disorder that affects the kidneys and eyes where it implicates the protein nephrin in the glomerular basement membrane leading to serious renal dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of cerebral cortex. It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons. Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface.

See full target information LAMB1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:14677 PubMed31605007

2019

Retinoic acid receptor α as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Rami M El Zein,Audrey H Soria,Jose Felipe Golib Dzib,Amanda J Rickard,Fabio L Fernandes-Rosa,Benoit Samson-Couterie,Isabelle Giscos-Douriez,Angélique Rocha,Marko Poglitsch,Celso E Gomez-Sanchez,Laurence Amar,Norbert B Ghyselinck,Arndt Benecke,Maria-Christina Zennaro,Sheerazed Boulkroun

Cell death & disease 6:e1584 PubMed25569101

2015

Candidate tumor suppressor B-cell translocation gene 3 impedes neoplastic progression by suppression of AKT.

Applications

Unspecified application

Species

Unspecified reactive species

Y-C Cheng,P-H Chen,H-Y Chiang,C-S Suen,M-J Hwang,T-Y Lin,H-C Yang,W-C Lin,P-L Lai,S-Y Shieh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin beta 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody (AB69633)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 1 antibody (AB69633)

Western blot - Anti-Laminin beta 1 antibody (AB69633)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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