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AB325915

Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal LAMB1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1

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Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)
  • sELISA

Lab

Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)

Interpolated concentration of native Laminin beta 1 was measured at different sample concentrations. Undiluted sample is as follows : human plasma (Citrate) 100%. Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)
  • sELISA

Lab

Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)

Sandwich ELISA with the capture antibody ab325915 dilution at 2 µg/mL and detector antibody ab325916 dilution at 0.5 µg/mL after biotin conjugation.

Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)
  • sELISA

Lab

Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)

Interpolated concentration of native Laminin beta 1 was measured at different sample concentrations. Undiluted sample is as follows : HepG2 supernatant (3 days) 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)
  • sELISA

Lab

Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)

Interpolated concentration of native Laminin beta 1 was measured at different sample concentrations. Undiluted samples are as follows : HCT116 cell extract (50 ug/mL), HepG2 cell extract (50 ug/mL) and HeLa cell extract (12.5 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)
  • sELISA

Lab

Sandwich ELISA - Anti-Laminin beta 1 antibody [EPR29489-505] - BSA and Azide free (Capture) (AB325915)

Interpolated concentrations of native Laminin beta 1 in human control wild type HeLa (human cervical adenocarcinoma epithelial cell) and LAMB1 (Laminin beta 1) knockout HeLa cell based on 12.5 ug/mL extract loads. The concentrations of Laminin beta 1 were measured in duplicate and interpolated from the Laminin beta 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Laminin beta 1 concentration was determined to be 163.7 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line ab255928) and undetectable in LAMB1 (Laminin beta 1) knockout HeLa extract (Human LAMB1 (Laminin beta 1) knockout HeLa cell line ab265414).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29489-505

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab325915 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Laminin beta 1. The recommended pair for sandwich ELISA is:

Capture: ab325915, Human Laminin beta 1 Capture Antibody (unconjugated)

Detector: ab325916, Human Laminin beta 1 Detector Antibody (unconjugated)

The reference range value is 31.3-2000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of the cerebral cortex (PubMed : 23472759). It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons (By similarity). Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface (By similarity). As a subunit of laminin-1 (also known as laminin-111 or EHS laminin), it is involved in the stimulation of agrin-induced receptor clustering through a MuSK-independent pathway (By similarity).
See full target information LAMB1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com