AB210956

Anti-Laminin beta 2 antibody [CL2979]

Name: Anti-Laminin beta 2 antibody [CL2979] (ab210956) | Abcam
Brand: Abcam Limited
SKU: AB210956

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(4 Publications)

Mouse Monoclonal Laminin beta 2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human LAMB2 aa 1250-1350.

View Alternative Names

LAMS, LAMB2, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemical analysis of paraffin-embedded human cervix tissue, labeling Laminin beta 2 using ab210956 at a 1/5000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemical analysis of paraffin-embedded human lymph node tissue (negative control), labeling Laminin beta 2 using ab210956 at a 1/5000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemical analysis of paraffin-embedded human stomach tissue, labeling Laminin beta 2 using ab210956 at a 1/5000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemical analysis of paraffin-embedded human colon tissue, labeling Laminin beta 2 using ab210956 at a 1/5000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemical analysis of paraffin-embedded human testis tissue, labeling Laminin beta 2 using ab210956 at a 1/5000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemical analysis of paraffin-embedded human heart tissue, labeling Laminin beta 2 using ab210956 at a 1/5000 dilution.

Supplier Data

Western blot - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

All lanes:

Western blot - Anti-Laminin beta 2 antibody [CL2979] (ab210956) at 1/500 dilution

All lanes:

Human lung tissue lysate

Predicted band size: 196 kDa

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Supplier Data

Western blot - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

All lanes:

Western blot - Anti-Laminin beta 2 antibody [CL2979] (ab210956) at 1/500 dilution

Lane 1:

Recombinant protein fragment of human Laminin 421

Lane 2:

Recombinant protein fragment of human Laminin 521

Lane 3:

Recombinant protein fragment of human Laminin 332

Lane 4:

Recombinant protein fragment of human Laminin 111

Predicted band size: 196 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2979

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LAMB2 aa 1250-1350. The exact immunogen used to generate this antibody is proprietary information.

P55268

Epitope

ab210956 binds to an epitope located within the peptide sequence NANHALSGLERDRLA (aa 1292-1306).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.

Biological function summary

Laminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.

Pathways

Laminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.

Laminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information LAMB2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical kidney journal 17:sfae037 PubMed38455522

2024

Characterization of glomerular basement membrane components within pediatric glomerular diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Chen,Xindi Zhou,Chun Gan,Qing Yang,Wanbing Chen,Xiaoqian Feng,Tao Zhang,Li Zhang,Lujun Dai,Yaxi Chen,Haiping Yang,Mo Wang,Wei Jiang,Qiu Li

Cells 11: PubMed35159261

2022

MicroRNA-100 Reduced Fetal Bovine Muscle Satellite Cell Myogenesis and Augmented Intramuscular Lipid Deposition by Modulating IGF1R.

Applications

Unspecified application

Species

Unspecified reactive species

Bilal Ahmad Mir,Elke Albrecht,Asghar Ali,Ola Hansson,Steffen Maak

Journal of the American Society of Nephrology : JASN 32:1713-1732 PubMed34049963

2021

Identification of an Altered Matrix Signature in Kidney Aging and Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Randles,Franziska Lausecker,Qingyang Kong,Hani Suleiman,Graeme Reid,Maria Kolatsi-Joannou,Bernard Davenport,Pinyuan Tian,Sara Falcone,Paul Potter,Tom Van Agtmael,Jill T Norman,David A Long,Martin J Humphries,Jeffrey H Miner,Rachel Lennon

Communications biology 4:161 PubMed33547385

2021

A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia Tang,Jimin Han,Sonal Dalvi,Kannan Manian,Lauren Winschel,Stefanie Volland,Celia A Soto,Chad A Galloway,Whitney Spencer,Michael Roll,Caroline Milliner,Vera L Bonilha,Tyler B Johnson,Lisa Latchney,Jill M Weimer,Erika F Augustine,Jonathan W Mink,Vamsi K Gullapalli,Mina Chung,David S Williams,Ruchira Singh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin beta 2 antibody [CL2979]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Western blot - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Western blot - Anti-Laminin beta 2 antibody [CL2979] (AB210956)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise