AB277530

Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Laminin beta 2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

LAMS, LAMB2, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta

2 Images

Lab

Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (AB277530)

This data was developed using ab277521, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 9273822 ).

Low expression : human liver (PMID : 7795887).

Exposure time : Lane 1 : 48 seconds

Lane 2-5 : 3minutes.

All lanes:

Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] (<a href='/en-us/products/primary-antibodies/laminin-beta-2-antibody-epr23568-91-ab277521'>ab277521</a>) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

Human placenta tissue lysate at 20 µg

Lane 5:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 196 kDa

Observed band size: 200 kDa

false

Lab

Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (AB277530)

This data was developed using ab277521, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 9273822).

Low expression : mouse liver, rat liver (PMID : 7795887)

Exposure time : Lane 1, 2, 4 : 3minutes

Lane 3 : 70 seconds.

All lanes:

Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] (<a href='/en-us/products/primary-antibodies/laminin-beta-2-antibody-epr23568-91-ab277521'>ab277521</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat heart tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 196 kDa

Observed band size: 200 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23568-91

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ImmunohistochemistryFloatingvibratomesections" : {"fullname" : "Immunohistochemistry (Floating vibratome sections)", "shortname":"Immunohistochemistry (Floating vibratome sections)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ImmunohistochemistryFloatingvibratomesections-species-checked": "notRecommended", "ImmunohistochemistryFloatingvibratomesections-species-dilution-info": "", "ImmunohistochemistryFloatingvibratomesections-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ImmunohistochemistryFloatingvibratomesections-species-checked": "notRecommended", "ImmunohistochemistryFloatingvibratomesections-species-dilution-info": "", "ImmunohistochemistryFloatingvibratomesections-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ImmunohistochemistryFloatingvibratomesections-species-checked": "notRecommended", "ImmunohistochemistryFloatingvibratomesections-species-dilution-info": "", "ImmunohistochemistryFloatingvibratomesections-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab277530 is the carrier-free version of ab277521.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.

Biological function summary

Laminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.

Pathways

Laminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.

Laminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information LAMB2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free

Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (AB277530)

Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (AB277530)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise