AB69632

Anti-Laminin gamma 1 antibody

Name: Anti-Laminin gamma 1 antibody (ab69632) | Abcam
Brand: Abcam Limited
SKU: AB69632

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(5 Publications)

Rabbit Polyclonal LAMC1 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human LAMC1.

View Alternative Names

LAMB2, LAMC1, Laminin subunit gamma-1, Laminin B2 chain, Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, Laminin-3 subunit gamma, Laminin-4 subunit gamma, Laminin-6 subunit gamma, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-9 subunit gamma, S-laminin subunit gamma, S-LAM gamma

1 Images

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Western blot - Anti-Laminin gamma 1 antibody (AB69632)

All lanes:

Western blot - Anti-Laminin gamma 1 antibody (ab69632) at 1/500 dilution

Lane 1:

extracts from HUVEC cells at 5 µg

Lane 2:

extracts from HUVEC cells at 5 µg with immunising peptide

Predicted band size: 178 kDa

Observed band size: >170 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human LAMC1. The exact immunogen used to generate this antibody is proprietary information.

P11047

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin gamma 1 also known as laminin-1 is an important component of the extracellular matrix. This protein has a molecular weight of about 200 kDa and is widely expressed in various tissues including epithelial and endothelial cells. Laminin gamma 1 is part of the larger laminin protein family which plays a significant role in the structural integrity of tissues. It primarily partners with other laminin chains to form a heterotrimeric complex that provides foundational support for cellular structures.

Biological function summary

Laminin gamma 1 contributes to cell adhesion differentiation and migration. It belongs to the laminin-1 complex serving as a scaffold for cells to interact with each other and with the extracellular matrix. This interaction facilitates cell signaling processes that influence cellular behavior. Additionally the laminin iHC (immunohistochemistry) technique often utilizes laminin gamma 1 to study cell-matrix interactions due to its involvement in these critical biological activities.

Pathways

Laminin gamma 1 is integral to basement membrane assembly and epithelial morphogenesis pathways. These pathways incorporate other key proteins such as integrins which mediate cell attachment to the laminin matrix. The pathway interactions involving laminin gamma 1 allow cells to respond effectively to mechanical and chemical stimuli thereby maintaining tissue homeostasis and promoting regenerative processes after injury.

Abnormalities in laminin gamma 1 expression or function associate with conditions like muscular dystrophy and certain cancers. Mutations affecting this protein can lead to compromised structural integrity and impaired cellular communication. In muscular dystrophy its relationship with proteins such as dystroglycan highlights the disruption in muscle cell adhesion and signaling. Similarly in cancer altered laminin gamma 1 expression affects tumor cell invasion and metastasis involving interactions with proteins such as matrix metalloproteinases (MMPs) which regulate extracellular matrix remodeling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. As a subunit of laminin-1 (also known as laminin-111 or EHS laminin), it is involved in the stimulation of agrin-induced receptor clustering through a MuSK-independent pathway.

See full target information LAMC1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Graefe's archive for clinical and experimental oph : PubMed32789677

2020

The novel mutation P36R in LRP5L contributes to congenital membranous cataract via inhibition of laminin γ1 and c-MAF.

Applications

Unspecified application

Species

Unspecified reactive species

Liyao Sun,Fanqian Song,Hanruo Liu,Chao Wang,Xianling Tang,Zhijian Li,Hongyan Ge,Ping Liu

Aging 11:2699-2723 PubMed31076560

2019

Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yan,Haiyang Yu,Liyao Sun,Hanruo Liu,Chao Wang,Xi Wei,Fanqian Song,Hulun Li,Hongyan Ge,Hua Qian,Xiaoguang Li,Xianling Tang,Ping Liu

Experimental eye research 180:174-183 PubMed30597146

2018

Kojic acid inhibits senescence of human corneal endothelial cells via NF-κB and p21 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wei,Dan Luo,Yu Yan,Haiyang Yu,Liyao Sun,Chao Wang,Fanqian Song,Hongyan Ge,Hua Qian,Xiaoguang Li,Xianling Tang,Ping Liu

Molecular medicine reports 17:5726-5733 PubMed29436687

2018

Laminins in an in vitro anterior lens capsule model established using HLE B-3 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yan,Hua Qian,Hongda Jiang,Haiyang Yu,Liyao Sun,Xi Wei,Yunduan Sun,Hongyan Ge,Haizhou Zhou,Xiaoguang Li,Takashi Hashimoto,Xianling Tang,Ping Liu

Nucleic acids research 40:3538-47 PubMed22187159

2011

Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion.

Applications

Species

Human

Gillian H Little,Houtan Noushmehr,Sanjeev K Baniwal,Benjamin P Berman,Gerhard A Coetzee,Baruch Frenkel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin gamma 1 antibody

Western blot - Anti-Laminin gamma 1 antibody (AB69632)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise