AB41823

Anti-Laminin gamma 1 antibody [BC17]

Name: Anti-Laminin gamma 1 antibody [BC17] (ab41823) | Abcam
Brand: Abcam Limited
SKU: AB41823
Price: 495 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal LAMC1 antibody. Suitable for IP, ELISA, IHC-Fr and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human LAMC1.

View Alternative Names

LAMB2, LAMC1, Laminin subunit gamma-1, Laminin B2 chain, Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, Laminin-3 subunit gamma, Laminin-4 subunit gamma, Laminin-6 subunit gamma, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-9 subunit gamma, S-laminin subunit gamma, S-LAM gamma

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BC17

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-Fr, IP, ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human LAMC1.

P11047

Specificity

The antibody recognizes the gamma 1 chain of human reduced and alkylated laminin produced by various cultured cells, which produce laminins complexed with it. The specificity of clone BC17 has been confirmed by immunoprecipitation and ELISA by using recombinant Laminin gamma 1.

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Reactivity data

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Product details

Balb/c spleen cells used to produce hybridoma.

The monoclonal antibody BC17 against Laminin gamma 1 can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing this chain. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning e.g. the integrity of the basement membranes in carcinomas.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.3 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin gamma 1 also known as laminin-1 is an important component of the extracellular matrix. This protein has a molecular weight of about 200 kDa and is widely expressed in various tissues including epithelial and endothelial cells. Laminin gamma 1 is part of the larger laminin protein family which plays a significant role in the structural integrity of tissues. It primarily partners with other laminin chains to form a heterotrimeric complex that provides foundational support for cellular structures.

Biological function summary

Laminin gamma 1 contributes to cell adhesion differentiation and migration. It belongs to the laminin-1 complex serving as a scaffold for cells to interact with each other and with the extracellular matrix. This interaction facilitates cell signaling processes that influence cellular behavior. Additionally the laminin iHC (immunohistochemistry) technique often utilizes laminin gamma 1 to study cell-matrix interactions due to its involvement in these critical biological activities.

Pathways

Laminin gamma 1 is integral to basement membrane assembly and epithelial morphogenesis pathways. These pathways incorporate other key proteins such as integrins which mediate cell attachment to the laminin matrix. The pathway interactions involving laminin gamma 1 allow cells to respond effectively to mechanical and chemical stimuli thereby maintaining tissue homeostasis and promoting regenerative processes after injury.

Abnormalities in laminin gamma 1 expression or function associate with conditions like muscular dystrophy and certain cancers. Mutations affecting this protein can lead to compromised structural integrity and impaired cellular communication. In muscular dystrophy its relationship with proteins such as dystroglycan highlights the disruption in muscle cell adhesion and signaling. Similarly in cancer altered laminin gamma 1 expression affects tumor cell invasion and metastasis involving interactions with proteins such as matrix metalloproteinases (MMPs) which regulate extracellular matrix remodeling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. As a subunit of laminin-1 (also known as laminin-111 or EHS laminin), it is involved in the stimulation of agrin-induced receptor clustering through a MuSK-independent pathway.

See full target information LAMC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Matrix biology : journal of the International Society for Matrix Biology 19:163-7 PubMed10842099

2000

Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin beta1 and gamma1 chains.

Applications

Unspecified application

Species

Unspecified reactive species

T Geberhiwot,Z Wondimu,S Salo,T Pikkarainen,J Kortesmaa,K Tryggvason,I Virtanen,M Patarroyo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin gamma 1 antibody [BC17]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise