Rabbit Recombinant Monoclonal Laminin gamma 3 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109029.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391)")
![Immunohistochemistry (Frozen sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--immunohistochemistry-frozen-sections-img18170.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391)")
![Flow Cytometry (Intracellular) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--flow-cytometry-intracellular-img2506.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45388.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22066.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (AB267391)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Mouse | Not recommended | Tested | Tested | Tested | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Alternative names
Laminin subunit gamma-3, Laminin-12 subunit gamma, Laminin-14 subunit gamma, Laminin-15 subunit gamma, Lamc3
Recommended products
Rabbit Recombinant Monoclonal Laminin gamma 3 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22699-151
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab267391 is the carrier-free version of Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Laminin gamma 3 often referred to as lam γ3 is a subunit of the laminin protein complex which plays an important role in the structure of the extracellular matrix. Alternate names include LAMC3. This protein has a molecular mass of approximately 180 kDa and is expressed in various tissues including the skin brain and reproductive systems. Laminin gamma 3 contributes to the structural integrity and organization of the basement membrane.
- Biological function summary
Laminin gamma 3 is involved in cell adhesion differentiation migration and survival. It integrates into laminin trimers forming part of the laminin-332 complex known for its role in hemidesmosome formation and epithelial cell anchorage. These interactions are critical for maintaining tissue architecture and function influencing processes such as wound healing and tissue repair.
- Pathways
Laminin gamma 3 operates within pathways that regulate cell-matrix interactions and intracellular signaling. It engages in the integrin and PI3K/AKT/mTOR pathways which are critical for cellular growth and survival. Laminin gamma 3 interacts with integrins such as α3β1 and α6β4 helping to transmit signals from the extracellular matrix to the cell interior therefore influencing cell behavior and fate.
- Associated diseases and disorders
Laminin gamma 3 has been linked to conditions such as skin blistering disorders and certain forms of muscular dystrophy. In skin disorders it interacts with proteins like collagen VII highlighting its role in maintaining the structural integrity of the skin. In muscular dystrophy improper laminin gamma 3 interactions can disrupt normal muscle function contributing to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109029.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Laminin gamma 3 with Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the basement membrane in the mouse lung is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429).
![Immunohistochemistry (Frozen sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--immunohistochemistry-frozen-sections-img18170.jpg "Immunohistochemistry (Frozen sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)")
Immunohistochemistry (Frozen sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling Laminin gamma 3 with Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429 at 1/100 (4.99 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429 ).
![Flow Cytometry (Intracellular) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--flow-cytometry-intracellular-img2506.jpg "Flow Cytometry (Intracellular) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)")
Flow Cytometry (Intracellular) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Laminin gamma 3 with Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429 at 1/50 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45388.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Laminin gamma 3 with Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the blood vessels in the mouse cerebrum (PMID:15824114) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391), expandable thumbnail](https://content.abcam.com/products/images/laminin-gamma-3-antibody-epr22699-151-bsa-and-azide-free-ab267391--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22066.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] - BSA and Azide free (ab267391)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Laminin gamma 3 with Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the basement membrane in the mouse testis (PMID: 15824114) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Laminin gamma 3 antibody [EPR22699-151] ab234429).
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