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Mouse Recombinant Monoclonal LAMP1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (AB302888), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (AB302888), expandable thumbnail
  • Western blot - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (AB302888), expandable thumbnail
  • Western blot - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (AB302888), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Also plays an important role in NK-cells cytotoxicity (PubMed:2022921, PubMed:23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (PubMed:7685349). (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Promotes also fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909). (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

Alternative names

CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1

Recommended products

Mouse Recombinant Monoclonal LAMP1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
1483CT517.45.23
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

Associated diseases and disorders

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (ab302888), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (ab302888)

    LAMP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human Kidney using mouse Anti-LAMP1 antibody

    This data was developed using Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LAMP1 with Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639 at 1/500 (1.992 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human kidney.The section was incubated with Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (ab302888), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (ab302888)

    LAMP1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human Liver using mouse Anti-LAMP1 antibody

    This data was developed using Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LAMP1 with Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639 at 1/500 (1.992 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human liver.The section was incubated with Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (ab302888), expandable thumbnail

    Western blot - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (ab302888)

    LAMP1 Western blot staining using mouse Anti-LAMP1 antibody

    This data was developed using Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBSFalse colour image of Western blot: Anti-LAMP1 antibody [1483CT517.45.23] (Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639) staining at 1/1000 dilution, shown in green; Rabbit anti-GAPDH antibody [16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639 was shown to bind specifically toLAMP1. A band was observed at 110 kDa in wild-type HAP1 cell lysates with no signal observed at this size in the LAMP1 knockout cell line. To generate this image, wild-type and LAMP1 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a PVDF-FL membrane. Membranes were blocked in Odyssey diluted in equal volume of 0.1 % TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution. Performed under reducing conditions.

    All lanes: Western blot - Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker (Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: LAMP1 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/10000 dilution

    Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 110 kDa

  • Western blot - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (ab302888), expandable thumbnail

    Western blot - Anti-LAMP1 antibody [1483CT517.45.23] (BSA and Azide free) (ab302888)

    LAMP1 Western blot staining using mouse Anti-LAMP1 antibody

    This data was developed using Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration: 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID: 27061067, 15111122). Exposure time: Lanes 1-3: 92 seconds
    Lanes 4-5: 37 seconds

    All lanes: Western blot - Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker (Anti-LAMP1 antibody [1483CT517.45.23] - Lysosome Marker ab302639) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: Human kidney tissue lysate at 10 µg

    Lane 5: Human spleen tissue lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 110 kDa

    Exposure time: 92s

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Product protocols

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