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Anti-LAMP1 antibody [1D4B] is a rat monoclonal antibody that is used to detect LAMP1 in Flow cytometry (Intra), IHC-P. Suitable for Human samples.

- Cited in over 210 publications
- Antibody clone 1D4B has been tried and trusted by researchers since 2005
- Kappa light chain isotype


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1D4B] (AB25245), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB25245), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 8.2
Constituents: 100% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing Lamp1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11438

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

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Target data

Function

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Also plays an important role in NK-cells cytotoxicity (PubMed:2022921, PubMed:23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (PubMed:7685349). (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Promotes also fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909). (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

Alternative names

CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1

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Anti-LAMP1 antibody [1D4B] is a rat monoclonal antibody that is used to detect LAMP1 in Flow cytometry (Intra), IHC-P. Suitable for Human samples.

- Cited in over 210 publications
- Antibody clone 1D4B has been tried and trusted by researchers since 2005
- Kappa light chain isotype

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing Lamp1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11438
Clone number
1D4B
Specificity

Murine CD107a/LAMP1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa)

Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-LAMP1 antibody [1D4B] (ab25245) is a rat monoclonal antibody and is validated for use in Flow Cyt (Intra), IHC-P in mouse samples.

Anti-LAMP1 antibody [1D4B] (ab25245) has been cited over 211 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-LAMP1 antibody [1D4B] (ab25245) has high sensitivity and specificity.

Anti-LAMP1 antibody [1D4B] (ab25245) has 16 independent reviews from customers.

Anti-LAMP1 antibody [1D4B] (ab25245) specifically detects LAMP1 (UniProt ID: P11279; Molecular weight: 42kDa) and is sold in 100 µg selling sizes.

Antibody clone 1D4B is also available pre-conjugated to FITC for your convenience (FITC Anti-LAMP1 antibody [1D4B] - Lysosome Marker ab24871).

LAMP1 (Lysosomal Associated Membrane Protein 1) is a protein integral to the lysosomal membrane, playing a significant role in lysosomal stability and cellular waste management. It is commonly used as a lysosome membrane marker in research. Alterations in LAMP1 expression have been implicated in several diseases, such as cancer, where it may aid in metastasis, and neurodegenerative diseases like Alzheimer's disease, where lysosomal dysfunction is a contributing factor.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

Associated diseases and disorders

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1D4B] (ab25245), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1D4B] (ab25245)

    IHC image of LAMP1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25245, 10µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.



    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (ab25245), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (ab25245)

    LAMP1 Flow Cytometry (Intracellular) staining using rat Anti-LAMP1 antibody

    Overlay histogram showing Jurkat cells stained with ab25245 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25245, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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