AB25245

Anti-LAMP1 antibody [1D4B] - Lysosome Marker

Name: Anti-LAMP1 antibody [1D4B] - lysosome marker (ab25245) | Abcam
Brand: Abcam Limited
SKU: AB25245
Price: 505 USD
Availability: InStock
Rating: 4 (20 reviews)

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What is this?

(20 Reviews)

(260 Publications)

Anti-LAMP1 antibody [1D4B] (ab25245) is a rat monoclonal antibody detecting LAMP1 in Flow Cytometry (Intra), IHC-P. Suitable for Human.

- Over 210 publications
- Trusted since 2005

View Alternative Names

CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB25245)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB25245)

IHC image of LAMP1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25245, 10µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB25245)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB25245)

Overlay histogram showing Jurkat cells stained with ab25245 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25245, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

519 FITC

FITC Anti-LAMP1 antibody [1D4B] - Lysosome Marker

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

1D4B

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

Cell preparation containing Lamp1 protein. The exact immunogen used to generate this antibody is proprietary information.

P11438

Specificity

Murine CD107a/LAMP1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Anti-LAMP1 antibody [1D4B] (ab25245) is a rat monoclonal antibody and is validated for use in Flow Cyt (Intra), IHC-P in mouse samples.

Anti-LAMP1 antibody [1D4B] (ab25245) has been cited over 211 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-LAMP1 antibody [1D4B] (ab25245) has high sensitivity and specificity.

Anti-LAMP1 antibody [1D4B] (ab25245) has 16 independent reviews from customers.

Anti-LAMP1 antibody [1D4B] (ab25245) specifically detects LAMP1 (UniProt ID: P11279; Molecular weight: 42kDa) and is sold in 100 µg selling sizes.

Antibody clone 1D4B is also available pre-conjugated to FITC for your convenience (ab24871).

LAMP1 (Lysosomal Associated Membrane Protein 1) is a protein integral to the lysosomal membrane, playing a significant role in lysosomal stability and cellular waste management. It is commonly used as a lysosome membrane marker in research. Alterations in LAMP1 expression have been implicated in several diseases, such as cancer, where it may aid in metastasis, and neurodegenerative diseases like Alzheimer's disease, where lysosomal dysfunction is a contributing factor.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 8.2 Constituents: 100% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed : 37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed : 37390818). Also plays an important role in NK-cells cytotoxicity (PubMed : 2022921, PubMed : 23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed : 23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed : 23847195). Presents carbohydrate ligands to selectins (PubMed : 7685349).. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed : 24970085, PubMed : 25972533, PubMed : 27605678, PubMed : 28448640). Also promotes fusion of the virus with host membrane in less acidic endosomes (PubMed : 29295909).. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

See full target information LAMP1

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Publications (260)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8170 PubMed40890115

2025

Isotope-encoded spatial biology identifies plaque-age-dependent maturation and synaptic loss in an Alzheimer's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jack I Wood,Maciej Dulewicz,Alicja Szadziewska,Sophia Weiner,Junyue Ge,Katie Stringer,Sneha Desai,Lydia Fenson,Diana Piotrowska,Gunnar Brinkmalm,Srinivas Koutarapu,Haady B Hajar,Kaj Blennow,Henrik Zetterberg,Damian M Cummings,Jeffrey N Savas,Frances A Edwards,Jörg Hanrieder

Nature communications 16:7994 PubMed40866328

2025

Microglial replacement in a Sandhoff disease mouse model reveals myeloid-derived β-hexosaminidase is necessary for neuronal health.

Applications

Unspecified application

Species

Unspecified reactive species

Kate I Tsourmas,Claire A Butler,Nellie E Kwang,Zachary R Sloane,Koby J G Dykman,Ghassan O Maloof,Biswa P Choudhury,Mousumi Paulchakrabarti,Christiana A Prekopa,Emily Z Tabaie,Robert P Krattli,Sanad M El-Khatib,Vivek Swarup,Munjal M Acharya,Lindsay A Hohsfield,Kim N Green

PloS one 20:e0329626 PubMed40748985

2025

Identification of the Francisella novicida FTN_0096 as a factor involved in intracellular replication and host response.

Applications

Unspecified application

Species

Unspecified reactive species

Dhandy Koesoemo Wardhana,Takashi Shimizu,Kenta Watanabe,Akihiko Uda,Masahisa Watarai

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze

Autophagy reports 4:2476218 PubMed40458442

2025

The TECPR1:ATG5-ATG12 complex conjugates LC3/ATG8 to damaged lysosomes that expose luminal glycans in response to osmotic imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxue Wang,Matthew Jefferson,Maria Ramos,Matthew Whelband,Kristin Kreuzer,Grace Khuu,Michael Lazarou,James Mccoll,James Lazenby,Cynthia B Whitchurch,Paul Verkade,Ulrike Mayer,Thomas Wileman

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70292 PubMed40457749

2025

Acute experimental colitis in 5xFAD Alzheimer's disease mice leads to enhanced monocyte infiltration into the brain accompanied by reduced β-amyloid deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Nanda K Navalpur Shanmugam,Frank Zamudio,Deepak K Vijaya Kumar,Kianna A Barrett,Ryan VanDoren,Meng Chen,Olivia M Barr,Sara Watson,Chih-Chung Jerry Lin,William A Eimer,Mehdi Jorfi,Se Hoon Choi,Robert D Moir,Rudolph E Tanzi

Aging cell 24:e70101 PubMed40346951

2025

SGLT2 Inhibition by Enavogliflozin Significantly Reduces Aβ Pathology and Restores Cognitive Function via Upregulation of Microglial AMPK Signaling in 5XFAD Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jihui Han,Jaehoon Song,Eun Sun Jung,Ji Won Choi,Hye Young Ji,Inhee Mook-Jung

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70231 PubMed40346446

2025

PLCG2 modulates TREM2 expression and signaling in response to Alzheimer's disease pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Evan J Messenger,Sydney A Baar,Logan M Bedford,Andy P Tsai,Peter Bor-Chian Lin,Chloe A Ferguson,Guixiang Xu,Abigail Wallace,Gary E Landreth,Bruce T Lamb,Stephanie J Bissel

Nature communications 16:3860 PubMed40274816

2025

Defective autophagy in CD4 T cells drives liver fibrosis via type 3 inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Rola Al Sayegh,Jinghong Wan,Charles Caër,Margot Azoulai,Maxime Gasperment,Sukriti Baweja,Marc-Anthony Chouillard,Janany Kandiah,Mathilde Cadoux,Morgane Mabire,Camille Pignolet,Tristan Thibault-Sogorb,Adel Hammoutene,Valérie Paradis,Loredana Saveanu,Rémy Nicolle,Hélène Gilgenkrantz,Emmanuel Weiss,Sophie Lotersztajn

Molecular neurodegeneration 20:9 PubMed39844286

2025

APOE Christchurch enhances a disease-associated microglial response to plaque but suppresses response to tau pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Kristine M Tran,Nellie E Kwang,Claire A Butler,Angela Gomez-Arboledas,Shimako Kawauchi,Cassandra Mar,Donna Chao,Rocio A Barahona,Celia Da Cunha,Kate I Tsourmas,Zechuan Shi,Shuling Wang,Sherilyn Collins,Amber Walker,Kai-Xuan Shi,Joshua A Alcantara,Jonathan Neumann,Duc M Duong,Nicholas T Seyfried,Andrea J Tenner,Frank M LaFerla,Lindsay A Hohsfield,Vivek Swarup,Grant R MacGregor,Kim N Green

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMP1 antibody [1D4B] - Lysosome Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB25245)

Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB25245)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (260)

Product promise