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Mouse Recombinant Monoclonal LAMP1 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (AB289548), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [25/Lamp-1] (AB289548), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (AB289548), expandable thumbnail
  • Western blot - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (AB289548), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Also plays an important role in NK-cells cytotoxicity (PubMed:2022921, PubMed:23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (PubMed:7685349). (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Promotes also fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909). (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

Alternative names

CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1

Recommended products

Mouse Recombinant Monoclonal LAMP1 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
25/Lamp-1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

Associated diseases and disorders

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (ab289548), expandable thumbnail

    Western blot - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (ab289548)

    LAMP1 Western blot staining using mouse Anti-LAMP1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27061067, 15111122).

    Exposure time: 3 seconds

    All lanes: Western blot - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (ab289548) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

    Lane 2: LAMP1 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 45 kDa

    Observed band size: 110 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [25/Lamp-1] (ab289548), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [25/Lamp-1] (ab289548)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling LAMP1 with ab289548 at 1/100 (10 μg/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image shows cytoplasmic staining in HeLa cell line. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab289548 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.

    Negative control 2: Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody at a 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (ab289548), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (ab289548)

    LAMP1 Flow Cytometry (Intracellular) staining using mouse Anti-LAMP1 antibody

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling LAMP1 with ab289548 at 1/1000 dilution (0.1 μg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti-Mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/5000 dilution was used as the secondary antibody.

  • Western blot - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (ab289548), expandable thumbnail

    Western blot - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (ab289548)

    LAMP1 Western blot staining using mouse Anti-LAMP1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27061067, 15111122).

    Exposure time: Lane 1, 4, 5: 26 seconds ; Lane 2, 3 : 70 seconds

    All lanes: Western blot - Anti-LAMP1 antibody [25/Lamp-1] - Lysosome Marker (ab289548) at 1/1000 dilution

    Lane 1: Human kidney tissue lysate at 20 µg

    Lane 2: Human spleen tissue lysate at 20 µg

    Lane 3: Human colon cancer tissue lysate at 20 µg

    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg

    Lane 5: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 45 kDa

    Observed band size: 110 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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