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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Knockout Tested Rabbit Recombinant Monoclonal LAMP1 antibody. Lysosome marker. Suitable for IHC-P, WB and reacts with Human samples. Cited in 15 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | ICC/IF | IP | Flow Cyt | WB | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/400 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.(Microbial infection) Acts as a receptor for Lassa virus protein.
Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1
Knockout Tested Rabbit Recombinant Monoclonal LAMP1 antibody. Lysosome marker. Suitable for IHC-P, WB and reacts with Human samples. Cited in 15 publications.
Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR4204
Affinity purification Protein A
Blue Ice
-20°C
Stable for 12 months at -20°C
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.
LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.
The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.
LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
LAMP1 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 27061067, 15111122).
All lanes: Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597) at 1/5000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20µg
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 20µg
Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 20µg
Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 110 kDa
Exposure time: 5s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LAMP1 with purified ab108597 at 1/400 dilution (0.36 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab108597 observed at 110 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108597 was shown to recognize LAMP1 in wild-type HAP1 cells as signal was lost at the expected MW in LAMP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LAMP1 knockout samples were subjected to SDS-PAGE. ab108597 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: LAMP1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: MCF7 whole cell lysate at 20 µg
Lane 4: Human Kidney whole cell lysate at 20 µg
Predicted band size: 45 kDa
Observed band size: 110 kDa
All lanes: Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: JAR cell lysate at 10 µg
Lane 3: Jurkat cell lysate at 10 µg
Lane 4: A431 cell lysate at 10 µg
Predicted band size: 45 kDa
Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using unpurified ab108597 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot: Anti-LAMP1 antibody [EPR4204] - Lysosome Marker staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108597 was shown to bind specifically to LAMP1. A band was observed at 100-120 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in LAMP1 knockout cell line. To generate this image, wild-type and LAMP1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597) at 1/1000 dilution
Lane 1: Wild-type HCT 116 cell lysate at 20 µg
Lane 2: LAMP1 knockout HCT 116 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: MCF7 cell lysate at 20 µg
Performed under reducing conditions.
Observed band size: 100-120 kDa
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human colon tissue staining CTBS with ab322065 at a 1:2000 (0.249 ug/ml) dilution, ab108597 anti-LAMP1 used at 1:400 (0.37 ug/ml) dilution.
Panel A: merged staining of anti-CTBS (green; Opal™520) and anti-LAMP1 (magenta; Opal™570) on human colon.
Panel B: anti-CTBS showed granular staining in human colon.
Panel C: anti-LAMP1 showed granular staining in human colon.
Panel D: Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab322065 and ab108597 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining CTBS with ab322065 at a 1:2000 (0.249 ug/ml) dilution, ab108597 anti-LAMP1 used at 1:400 (0.37 ug/ml) dilution.
Panel A: merged staining of anti-CTBS (green; Opal™520) and anti-LAMP1 (magenta; Opal™570) on human liver.
Panel B: anti-CTBS showed granular staining in human liver.
Panel C: anti-LAMP1 showed granular staining in human liver.
Panel D: Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab322065 and ab108597 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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