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Knockout Tested Rabbit Recombinant Monoclonal LAMP1 antibody. Lysosome marker. Suitable for IHC-P, WB and reacts with Human samples. Cited in 15 publications.


Images

Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597), expandable thumbnail
  • Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597), expandable thumbnail
  • Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (AB108597), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/400
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed:37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Also plays an important role in NK-cells cytotoxicity (PubMed:2022921, PubMed:23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed:23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed:23847195). Presents carbohydrate ligands to selectins (PubMed:7685349). (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:28448640). Promotes also fusion of the virus with host membrane in less acidic endosomes (PubMed:29295909). (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

Alternative names

CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal LAMP1 antibody. Lysosome marker. Suitable for IHC-P, WB and reacts with Human samples. Cited in 15 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4204
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

Associated diseases and disorders

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597), expandable thumbnail

    Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)

    LAMP1 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 27061067, 15111122).

    All lanes: Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/5000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20µg

    Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 20µg

    Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 20µg

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa

    Observed band size: 110 kDa

    Exposure time: 5s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LAMP1 with purified ab108597 at 1/400 dilution (0.36 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597), expandable thumbnail

    Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)

    Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab108597 observed at 110 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab108597 was shown to recognize LAMP1 in wild-type HAP1 cells as signal was lost at the expected MW in LAMP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LAMP1 knockout samples were subjected to SDS-PAGE. ab108597 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: LAMP1 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: MCF7 whole cell lysate at 20 µg

    Lane 4: Human Kidney whole cell lysate at 20 µg

    Predicted band size: 45 kDa

    Observed band size: 110 kDa

  • Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597), expandable thumbnail

    Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)

    All lanes: Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: JAR cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: A431 cell lysate at 10 µg

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)

    Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using unpurified ab108597 at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597), expandable thumbnail

    Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)

    Western blot: Anti-LAMP1 antibody [EPR4204] - Lysosome Marker staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108597 was shown to bind specifically to LAMP1. A band was observed at 100-120 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in LAMP1 knockout cell line. To generate this image, wild-type and LAMP1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/1000 dilution

    Lane 1: Wild-type HCT 116 cell lysate at 20 µg

    Lane 2: LAMP1 knockout HCT 116 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Performed under reducing conditions.

    Observed band size: 100-120 kDa

  • Multiplex immunohistochemistry - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597), expandable thumbnail

    Multiplex immunohistochemistry - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human colon tissue staining CTBS with ab322065 at a 1:2000 (0.249 ug/ml) dilution, ab108597 anti-LAMP1 used at 1:400 (0.37 ug/ml) dilution.

    Panel A: merged staining of anti-CTBS (green; Opal™520) and anti-LAMP1 (magenta; Opal™570) on human colon.

    Panel B: anti-CTBS showed granular staining in human colon.

    Panel C: anti-LAMP1 showed granular staining in human colon.

    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab322065 and ab108597 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597), expandable thumbnail

    Multiplex immunohistochemistry - Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining CTBS with ab322065 at a 1:2000 (0.249 ug/ml) dilution, ab108597 anti-LAMP1 used at 1:400 (0.37 ug/ml) dilution.

    Panel A: merged staining of anti-CTBS (green; Opal™520) and anti-LAMP1 (magenta; Opal™570) on human liver.

    Panel B: anti-CTBS showed granular staining in human liver.

    Panel C: anti-LAMP1 showed granular staining in human liver.

    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab322065 and ab108597 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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