AB13523

Anti-LAMP1 antibody [LY1C6] - Lysosome Marker

Name: Anti-LAMP1 antibody [LY1C6] - Lysosome Marker (ab13523) | Abcam
Brand: Abcam Limited
SKU: AB13523
Price: 535 USD
Availability: InStock
Rating: 1 (3 reviews)

(3 Reviews)

(27 Publications)

Mouse Monoclonal LAMP1 antibody. Suitable for ICC, WB and reacts with Human, Rat samples. Cited in 27 publications.

View Alternative Names

CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1

3 Images

Supplier Data

Immunocytochemistry - Anti-LAMP1 antibody [LY1C6] - Lysosome Marker (AB13523)

ab13523 at 1/100 dilution staining LAMP1 in LAMP1 transfected HeLa cells by Immunocytochemistry/ immunofluorescence. A Alexa Fluor® 568 conjugated goat anti mouse was used at 1/50 dilution as secondary.

Supplier Data

Western blot - Anti-LAMP1 antibody [LY1C6] - Lysosome Marker (AB13523)

All lanes:

Western blot - Anti-LAMP1 antibody [LY1C6] - Lysosome Marker (ab13523) at 1/1000 dilution

All lanes:

Rat liver microsome lysate at 15 µg

Secondary

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Sheep Anti-Mouse IgG: HRP

Predicted band size: 45 kDa

false

PubMed

Western blot - Anti-LAMP1 antibody [LY1C6] - Lysosome Marker (AB13523)

ab13523 used in Western Blot. Lysates prepared from CHO cells stably expressing aIIbß3 integrins (A5 cells) were used.

WCL, N/IC, C, P, and PM indicate whole cell lysates, nuclear/intact cell, cytosolic, high-speed pellet, and plasma membrane fractions, respectively.

All lanes:

Western blot - Anti-LAMP1 antibody [LY1C6] - Lysosome Marker (ab13523)

Predicted band size: 45 kDa

false

Image from Banno A et al, J Biol Chem. 2012 Apr 20;287(17):13799-812. Epub 2012 Feb 18, Fig 4. DOI 10.1074/jbc.M112.341214 April 20, 2012 The Journal of Biological Chemistry, 287, 13799-13812.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LY1C6

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

ICC, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

For maximum product recovery centrifuge the product vial before removing cap.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed : 37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed : 37390818). Also plays an important role in NK-cells cytotoxicity (PubMed : 2022921, PubMed : 23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed : 23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed : 23847195). Presents carbohydrate ligands to selectins (PubMed : 7685349).. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed : 24970085, PubMed : 25972533, PubMed : 27605678, PubMed : 28448640). Also promotes fusion of the virus with host membrane in less acidic endosomes (PubMed : 29295909).. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

See full target information LAMP1

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36078149

2022

Around-the-Clock Noise Induces AD-like Neuropathology by Disrupting Autophagy Flux Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfang Zheng,Xiaojun She,Chunping Wang,Yingwen Zhu,Bo Fu,Kefeng Ma,Honglian Yang,Xiujie Gao,Xiaofang Li,Fangshan Wu,Bo Cui

Scientific reports 11:10411 PubMed34002002

2021

Development and characterization of a monoclonal antibody blocking human TRPM4 channel.

Applications

Unspecified application

Species

Unspecified reactive species

See Wee Low,Yahui Gao,Shunhui Wei,Bo Chen,Bernd Nilius,Ping Liao

Virology 557:23-33 PubMed33601113

2021

Rat and human cytomegalovirus ORF116 encodes a virion envelope glycoprotein required for infectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Philippe Gatault,Iris K A Jones,Christine Meyer,Craig Kreklywich,Timothy Alexander,Patricia P Smith,Michael Denton,Josh Powell,Susan L Orloff,Daniel N Streblow

Cell death & disease 11:554 PubMed32694519

2020

Autophagy is involved in the protective effect of p21 on LPS-induced cardiac dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Sihui Huang,Man Xu,Libo Liu,Jingjing Yang,Huibo Wang,Chunxia Wan,Wei Deng,Qizhu Tang

OncoTargets and therapy 13:401-411 PubMed32021285

2020

PHLPP Sensitizes Multiple Myeloma Cells to Bortezomib Through Regulating LAMP2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Liu,Chengyuan Li,Yunfeng Fu,Jing Liu

Neurobiology of disease 129:182-194 PubMed31121321

2019

V232M substitution restricts a distinct O-glycosylation of PLD3 and its neuroprotective function.

Applications

Unspecified application

Species

Unspecified reactive species

Atanas Vladimirov Demirev,Ha-Lim Song,Mi-Hyang Cho,Kwangmin Cho,Jong-Jin Peak,Hyun Ju Yoo,Dong-Hou Kim,Seung-Yong Yoon

Cell research 29:460-473 PubMed30948787

2019

Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zong,Chen-Song Zhang,Mengqi Li,Wen Wang,Zhichao Wang,Simon A Hawley,Teng Ma,Jin-Wei Feng,Xiao Tian,Qu Qi,Yu-Qing Wu,Cixiong Zhang,Zhiyun Ye,Shu-Yong Lin,Hai-Long Piao,D Grahame Hardie,Sheng-Cai Lin

Molecular and cellular neurosciences 95:13-24 PubMed30594669

2018

Ouabain activates transcription factor EB and exerts neuroprotection in models of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ha-Lim Song,Atanas Vladimirov Demirev,Na-Young Kim,Dong-Hou Kim,Seung-Yong Yoon

Cancer immunology research 6:1057-1068 PubMed30018046

2018

Mechanisms by Which Dendritic Cells Present Tumor Microparticle Antigens to CD8 T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Ma,Keke Wei,Huafeng Zhang,Ke Tang,Fei Li,Tianzhen Zhang,Junwei Liu,Pingwei Xu,Yuandong Yu,Weiwei Sun,LiYan Zhu,Jie Chen,Li Zhou,Xiaoyu Liang,Jiadi Lv,Roland Fiskesund,Yuying Liu,Bo Huang

Oncotarget 7:76238-76249 PubMed27780926

2016

Resistance of glioma cells to nutrient-deprived microenvironment can be enhanced by CD133-mediated autophagy.

Applications

Species

Rat

Haojie Sun,Mingzhi Zhang,Kai Cheng,Peng Li,Shuo Han,Ruizhi Li,Ming Su,Wotan Zeng,Jinwen Liu,Jinhai Guo,Yinan Liu,Xiaoyan Zhang,Qihua He,Li Shen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMP1 antibody [LY1C6] - Lysosome Marker

Immunocytochemistry - Anti-LAMP1 antibody [LY1C6] - Lysosome Marker (AB13523)

Western blot - Anti-LAMP1 antibody [LY1C6] - Lysosome Marker (AB13523)

Western blot - Anti-LAMP1 antibody [LY1C6] - Lysosome Marker (AB13523)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (27)

Product promise